公开(公告)号：US08048652B2

公开(公告)日：2011-11-01

申请号：US11433752

申请日：2006-05-12

申请人： Jaouad Fichtali ,  Micah Hazzy Needham ,  Henry Linsert, Jr.

发明人： Jaouad Fichtali ,  Micah Hazzy Needham ,  Henry Linsert, Jr.

IPC分类号： C12P7/64

CPC分类号： C12P1/00 ,  A23J3/347 ,  A23L33/12 ,  A23L33/135 ,  A23L33/18 ,  A23V2002/00 ,  A61K8/361 ,  A61K8/97 ,  A61Q19/00 ,  C12P7/6427 ,  A23V2250/1882

摘要： The present invention includes a palatable, stable composition comprising a biomass hydrolysate emulsion for incorporation, into, or used as, nutritional products, cosmetic products or pharmaceutical products. Preferred sources for biomass are microbial sources, plant sources and animal sources. The present invention also provides methods for making such compositions, specifically, a method for producing a product comprising a nutrient, particularly a long chain polyunsaturated fatty acid, comprising hydrolyzing a biomass comprising the nutrient and emulsifying the hydrolyzed biomass. Such compositions and methods are useful, for example, for increasing intake of nutrients such as omega-3 long chain polyunsaturated fatty acids having 18 or more carbons.

摘要翻译： 本发明包括一种可口的稳定的组合物，其包含用于掺入或用作营养产品，化妆品或药物产品的生物质水解产物乳液。 生物质的优选来源是微生物来源，植物来源和动物来源。 本发明还提供了制备这种组合物的方法，具体地说，涉及生产包含营养素，特别是长链多不饱和脂肪酸的产品的方法，包括水解包含营养物质的生物质并乳化水解的生物质。 这样的组合物和方法可用于例如增加摄入营养物质，例如具有18个或更多个碳的ω-3长链多不饱和脂肪酸。

公开(公告)号：US07824892B2

公开(公告)日：2010-11-02

申请号：US11738425

申请日：2007-04-20

申请人： Paul W. Behrens ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

发明人： Paul W. Behrens ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC分类号： C12P7/64 ,  C12N1/12 ,  C11B1/00 ,  A23D9/00 ,  A23L1/30

CPC分类号： C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：US20080032353A1

公开(公告)日：2008-02-07

申请号：US11738414

申请日：2007-04-20

申请人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

发明人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC分类号： C12P7/64 ,  C12N1/12

CPC分类号： C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：US20060100279A1

公开(公告)日：2006-05-11

申请号：US11313524

申请日：2005-12-20

申请人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

发明人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC分类号： C12P7/64 ,  A61K31/202

CPC分类号： C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：US07745183B2

公开(公告)日：2010-06-29

申请号：US11738414

申请日：2007-04-20

申请人： Paul W. Behrens ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

发明人： Paul W. Behrens ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC分类号： C12P7/64 ,  C12N1/12 ,  A23D9/00 ,  A23L1/30 ,  C11B1/00

CPC分类号： C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

摘要翻译： 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：US07678931B2

公开(公告)日：2010-03-16

申请号：US11255787

申请日：2005-10-21

申请人： Jaouad Fichtali ,  Anand Sundararajan

发明人： Jaouad Fichtali ,  Anand Sundararajan

IPC分类号： C11B1/00

CPC分类号： C12N1/14 ,  A23L33/12 ,  A61K36/00 ,  A61K36/062 ,  C11B1/02 ,  C11B1/10 ,  C12N1/005 ,  C12N1/12

摘要： The present invention relates to a process for preparing a biomass, such as from a microbial fermentation, for an extraction process to separate desired chemicals, nutritional products, bioactive components, proteins, carbohydrates, and lipids, from the biomass. Particularly preferred substances to extract include docosahexaenoic acid, docosapentaenoic acid, and arachidonic acid. The present invention also includes extracting the prepared biomass. Biomasses to be treated in accordance with the methods of the invention include plant, animal, and microbial biomass, particularly a microorganism such as Crypthecodinium cohnii and a fungus such as Mortierella alpina.

摘要翻译： 本发明涉及从微生物发酵制备生物质的方法，用于从生物质中分离所需化学品，营养产品，生物活性成分，蛋白质，碳水化合物和脂质的提取方法。 特别优选的提取物质包括二十二碳六烯酸，二十二碳五烯酸和花生四烯酸。 本发明还包括提取制备的生物质。 根据本发明的方法处理的生物质包括植物，动物和微生物生物质，特别是微生物如隐孢子虫（Crypthecodinium cohnii）和真菌如高山被孢霉（Mortierella alpina）。

公开(公告)号：US20080044866A1

公开(公告)日：2008-02-21

申请号：US11738430

申请日：2007-04-20

申请人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

发明人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC分类号： C12P7/64

CPC分类号： C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

公开(公告)号：US20080032356A1

公开(公告)日：2008-02-07

申请号：US11738422

申请日：2007-04-20

申请人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

发明人： Paul Behrens ,  John Thompson ,  Kirk Apt ,  Joseph Pfeifer ,  James Wynn ,  James Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC分类号： C12P7/64 ,  C12N1/12

CPC分类号： C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

公开(公告)号：US20060122410A1

公开(公告)日：2006-06-08

申请号：US11255787

申请日：2005-10-21

申请人： Jaouad Fichtali ,  Anand Sundararajan

发明人： Jaouad Fichtali ,  Anand Sundararajan

IPC分类号： C11B1/00

CPC分类号： C12N1/14 ,  A23L33/12 ,  A61K36/00 ,  A61K36/062 ,  C11B1/02 ,  C11B1/10 ,  C12N1/005 ,  C12N1/12

摘要： The present invention relates to a process for preparing a biomass, such as from a microbial fermentation, for an extraction process to separate desired chemicals, nutritional products, bioactive components, proteins, carbohydrates, and lipids, from the biomass. Particularly preferred substances to extract include docosahexaenoic acid, docosapentaenoic acid, and arachidonic acid. The present invention also includes extracting the prepared biomass. Biomasses to be treated in accordance with the methods of the invention include plant, animal, and microbial biomass, particularly a microorganism such as Crypthecodinium cohnii and a fungus such as Mortierella alpina.

摘要翻译： 本发明涉及从微生物发酵制备生物质的方法，用于从生物质中分离所需化学品，营养产品，生物活性成分，蛋白质，碳水化合物和脂质的提取方法。 特别优选的提取物质包括二十二碳六烯酸，二十二碳五烯酸和花生四烯酸。 本发明还包括提取制备的生物质。 根据本发明的方法处理的生物质包括植物，动物和微生物生物质，特别是微生物如隐孢子虫（Crypthecodinium cohnii）和真菌如高山被孢霉（Mortierella alpina）。

公开(公告)号：US5855688A

公开(公告)日：1999-01-05

申请号：US824304

申请日：1997-03-26

申请人： Jaouad Fichtali ,  Yaw J. Owusu-Ansah ,  Peter Chang

发明人： Jaouad Fichtali ,  Yaw J. Owusu-Ansah ,  Peter Chang

IPC分类号： C08B30/04

CPC分类号： C08B30/04

摘要： A high quality starch, wholesome to be used for food applications is produced from green unpeeled bananas though a cost efficient, commercially viable process. The process steps involve an optional initial comminution, lye milling of the bananas, holding the milled solution for a minimum of 1 hour, sieving to remove pomace and fiber, concentrating, neutralizing the starch solution, dewatering and drying. The final product is of very high quality and devoid of detectable pesticide and/or fungicide residues.

摘要翻译： 有益于用于食品应用的高质量淀粉是通过绿色未剥皮香蕉生产的，但经济有效，商业上可行的方法。 工艺步骤包括可选的初始粉碎，香蕉的碱液碾磨，将研磨的溶液保持至少1小时，筛分除去果渣和纤维，浓缩，中和淀粉溶液，脱水和干燥。 最终产品质量非常高，没有可检测的农药和/或杀真菌剂残留物。