Strong early seed-specific gene regulatory region
    7.
    发明授权
    Strong early seed-specific gene regulatory region 有权
    强大的早期种子特异性基因调控区

    公开(公告)号:US06437220B1

    公开(公告)日:2002-08-20

    申请号:US09352125

    申请日:1999-07-13

    IPC分类号: C12N1529

    摘要: Nucleic acid sequences and methods for their use are described which provide for early seed-specific transcription, in order to modulate or modify expression of foreign or endogenous genes in seeds, particularly embryo cells. The method finds particular use in conjunction with modifying fatty acid production in seed tissue.

    摘要翻译: 描述了用于其使用的核酸序列及其方法,其提供早期种子特异性转录,以调节或修饰种子,特别是胚胎细胞中外源或内源基因的表达。 该方法特别用于改变种子组织中的脂肪酸生产。

    Structure and expression of the biotin carboxylase subunit of heteromeric acetyl-CoA carboxylase
    8.
    发明授权
    Structure and expression of the biotin carboxylase subunit of heteromeric acetyl-CoA carboxylase 失效
    异聚乙酰辅酶A羧化酶的生物素羧化酶亚基的结构和表达

    公开(公告)号:US06218600B1

    公开(公告)日:2001-04-17

    申请号:US08662344

    申请日:1996-06-12

    IPC分类号: A01H500

    摘要: The gene encoding a biotin carboxylase subunit of heteromeric ACCase has been isolated and sequenced. The cDNA sequence and deduced amino acid sequence is set forth in FIG. 1 and has been accorded GenBank Accession No. L38260. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis in plants and seeds which depends on malonyl-CoA in the plastid may also be controlled.

    摘要翻译: 已经分离并测序了编码异聚ACCase的生物素羧化酶亚基的基因。 cDNA序列和推导的氨基酸序列如图1所示。 1,已获得GenBank登录号L38260。 通过控制本发明的基因的表达,可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,通过在正义或反义方向引入本发明基因的构建体,可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,也可以控制依赖于质体中丙二酰辅酶A的植物和种子中的脂肪酸合成。