公开(公告)号：US07700348B2

公开(公告)日：2010-04-20

申请号：US10483379

申请日：2002-07-05

申请人： Jean René Martin ,  Michel Garrigues ,  Marlène Bras ,  Francois Bessueille ,  Eliane Souteyrand ,  Michel Cabrera ,  Jean-Pierre Cloarec ,  Jean-Paul Chauvet ,  Dorothée Chauvet, legal representative ,  Jean-Michel Chauvet, legal representative ,  Christophe Chauvet, legal representative

发明人： Jean René Martin ,  Michel Garrigues ,  Marlène Bras ,  Francois Bessueille ,  Eliane Souteyrand ,  Michel Cabrera ,  Jean-Pierre Cloarec ,  Jean-Paul Chauvet

IPC分类号： C12M1/34

CPC分类号： B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B82Y30/00 ,  G01N21/645 ,  G01N33/54353 ,  G01N33/54373 ,  G01N2021/6463

摘要： The invention relates to a biochip comprising a substrate presenting a reflecting main surface and wherein the main surface presents localized sites that are made sensitive to fluorescence detection by a transparent layer having optical thickness (2k+1)λ/4 where k is a positive or zero integer and where λ designates a wavelength lying between a wavelength λ0 at which fluorescence is excited and a wavelength λ1 at which fluorescence is emitted. The biochip can be manufactured by depositing a transparent layer of thickness mλ′/2 on a substrate and by making studs or wells of thickness (2k+1)λ/4 in said layer to form said sensitive sites.

摘要翻译： 本发明涉及一种生物芯片，其包括具有反射主表面的基底，并且其中主表面具有对具有光学厚度（2k + 1）λ/ 4的透明层对荧光检测敏感的局部位置，其中k为正或 零整数，其中λ表示位于激发荧光的波长λ0和发射荧光的波长λ1之间的波长。 生物芯片可以通过在衬底上沉积厚度为mλ'/ 2的透明层并且在所述层中制作厚度为（2k + 1）λ/ 4的螺柱或孔以形成所述敏感部位来制造。