公开(公告)号：US20090069193A1

公开(公告)日：2009-03-12

申请号：US12200894

申请日：2008-08-28

申请人： Jeb H. Flemming ,  Colin T. Buckley ,  Carrie F. Schmidt

发明人： Jeb H. Flemming ,  Colin T. Buckley ,  Carrie F. Schmidt

IPC分类号： C40B30/04 ,  C40B40/00 ,  G03F7/20 ,  B05D3/10 ,  C40B50/18

CPC分类号： G01N33/552 ,  G01N33/553

摘要： The present invention provides a method of forming one or more biological-binding areas on a substrate for biological-testing. The method includes activating at least a portion of a glass-ceramic substrate comprising glass and one or more metal containing compounds. The one or more metal containing compounds have a range of diameters that are less than about 300 nanometers in diameter and are spaced an average distance of at least one-half the midpoint of the diameter range apart. The one or more metals include compounds selected from metal oxides, metal nanoparticles, metal alloys, and atomic metals. The glass-ceramic substrate is heated to a temperature near the glass transformation temperature to form one or more metal nanoparticles in one or more ceramic biological-binding areas. The glass-ceramic substrate is etched to expose one or more metal. One or more biological molecules are contacted with one or more ceramic biological-binding areas to provide one or more biological testing areas with an increased binding area as compared to un-activated areas.

摘要翻译： 本发明提供了在用于生物测试的底物上形成一个或多个生物结合区域的方法。 该方法包括激活包含玻璃和一种或多种含金属化合物的玻璃 - 陶瓷衬底的至少一部分。 一种或多种含金属化合物的直径范围小于直径约300纳米，并且间隔开至少两分之一直径中点的平均距离。 一种或多种金属包括选自金属氧化物，金属纳米颗粒，金属合金和原子金属的化合物。 将玻璃陶瓷基板加热至接近玻璃化转变温度的温度，以在一个或多个陶瓷生物结合区域中形成一种或多种金属纳米颗粒。 蚀刻玻璃陶瓷衬底以暴露一种或多种金属。 一个或多个生物分子与一个或多个陶瓷生物结合区域接触，以提供与未激活区域相比具有增加的结合面积的一个或多个生物测试区域。