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公开(公告)号：US20130210638A1
公开(公告)日：2013-08-15
申请号：US13762843
申请日：2013-02-08
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2525/155 , C12Q2525/191 , C12Q2563/159 , C12Q2565/629
摘要： The invention generally relates to methods for sequencing a nucleic acid template from both a 5′ and a 3′ end in a single sequencing reaction. In certain embodiments, methods of the invention involve amplifying a nucleic acid template to produce a plurality of amplicons, splitting the amplicons into first and second portions, attaching a first oligonucleotide including a first universal primer site to a 5′ end of the amplicons in the first portion, and attaching a second oligonucleotide including a second universal primer site to a 3′ end of the amplicons in the second portion. The first and second primer sites may be the same or may be different. The method additionally involves pooling the first and second portions, and sequencing the pooled amplicons, thereby sequencing a nucleic acid template from both a 5′ and a 3′ end in a single sequencing reaction.
摘要翻译： 本发明一般涉及在单次测序反应中从5'和3'末端测序核酸模板的方法。 在某些实施方案中，本发明的方法包括扩增核酸模板以产生多个扩增子，将扩增子分裂成第一和第二部分，将包含第一通用引物位点的第一寡核苷酸连接到扩增子的5'末端 第一部分，并且在第二部分中将包含第二通用引物位点的第二寡核苷酸连接到扩增子的3'末端。 第一和第二引物位点可以相同或可以不同。 该方法另外涉及合并第一和第二部分，并对合并的扩增子进行测序，从而在单个测序反应中从5'和3'末端测序核酸模板。
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公开(公告)号：US20130210659A1
公开(公告)日：2013-08-15
申请号：US13764459
申请日：2013-02-11
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6844 , C12Q1/6883 , C12Q1/6886 , C12Q2600/156 , C12Q2525/155 , C12Q2525/161 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629 , C12Q2521/319 , C12Q2521/501 , C12Q2545/101
摘要： The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.
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公开(公告)号：US20120220494A1
公开(公告)日：2012-08-30
申请号：US13398677
申请日：2012-02-16
CPC分类号： C12N15/1075 , C12Q1/6874 , C40B50/08 , G01N33/5436 , G01N33/58
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
摘要翻译： 本发明提供条形码文件库及其制备和使用方法，包括获得多个核酸构建体，其中每个构建体包含独特的N-聚体和功能性N-聚体，并将构建体分离成流体隔室，使得每个隔室含有一个 或更多的独特构造的副本。 本发明还提供数字PCR和数字PCR中条形码库的使用方法。
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公开(公告)号：US09150852B2
公开(公告)日：2015-10-06
申请号：US13398677
申请日：2012-02-16
IPC分类号： C40B50/08 , C12N15/10 , G01N33/543 , G01N33/58
CPC分类号： C12N15/1075 , C12Q1/6874 , C40B50/08 , G01N33/5436 , G01N33/58
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
摘要翻译： 本发明提供条形码文件库及其制备和使用方法，包括获得多个核酸构建体，其中每个构建体包含独特的N-聚体和功能性N-聚体，并将构建体分离成流体隔室，使得每个隔室含有一个 或更多的独特构造的副本。 本发明还提供数字PCR和数字PCR中条形码库的使用方法。
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公开(公告)号：US09399797B2
公开(公告)日：2016-07-26
申请号：US13460762
申请日：2012-04-30
CPC分类号： C12Q1/686 , C12Q2565/629
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.
摘要翻译： 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，提供了用于测定样品的核酸组成的方法。
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公开(公告)号：US20120302448A1
公开(公告)日：2012-11-29
申请号：US13460762
申请日：2012-04-30
CPC分类号： C12Q1/686 , C12Q2565/629
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.
摘要翻译： 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，提供了用于测定样品的核酸组成的方法。
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