公开(公告)号：US20240352507A1

公开(公告)日：2024-10-24

申请号：US18606987

申请日：2024-03-15

申请人： Roche Sequencing Solutions, Inc.

发明人： Janine Mok ,  Ulrich Schlecht ,  Austin So

IPC分类号： C12Q1/6811 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C40B40/06 ,  C40B50/08 ,  C40B80/00

CPC分类号： C12Q1/6811 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C40B40/06 ,  C40B50/08 ,  C40B80/00

摘要： The invention comprises a method and compositions for sequencing library preparation, which increases the throughput of single-molecule sequencing (SMS) platforms by generating long concatenated templates from pools of short DNA molecules.

公开(公告)号：US11965877B2

公开(公告)日：2024-04-23

申请号：US14874553

申请日：2015-10-05

申请人： Bio-Rad Laboratories, Inc.

发明人： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC分类号： G01N33/53 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US11815475B2

公开(公告)日：2023-11-14

申请号：US16490710

申请日：2018-02-28

申请人： THE UNIVERSITY OF TOKYO

发明人： Makoto Fujita ,  Daishi Fujita ,  Yuya Domoto ,  Hiroki Takezawa

IPC分类号： G01N23/20 ,  G01N23/205 ,  G01N23/2055 ,  G01N23/207 ,  G01N23/20008 ,  C30B7/04 ,  C40B30/10 ,  C40B50/08 ,  C40B40/16 ,  G01N23/20091

CPC分类号： G01N23/2055 ,  C30B7/04 ,  C40B30/10 ,  C40B40/16 ,  C40B50/08 ,  G01N23/20 ,  G01N23/205 ,  G01N23/207 ,  G01N23/20008 ,  G01N23/2073 ,  G01N23/20091 ,  G01N2223/056 ,  G01N2223/106 ,  G01N2223/1016 ,  G01N2223/602

摘要： The present invention provides a novel method for identifying a molecular structure by a single crystal X-ray analysis. A single crystal that gives an X-ray diffraction spectrum sufficient for determining a structure of a molecule can be efficiently obtained by including a test molecule in a metal complex, and then crystallizing the test-molecule included in the metal complex. By analyzing this single crystal by an X-ray analysis, it is possible to determine a structure of the test molecule without obtaining a single crystal of the test molecule. With the novel method according to the present invention, the structure of a test molecule in a trace amount of a sample can also be determined.

公开(公告)号：US20230219043A1

公开(公告)日：2023-07-13

申请号：US18178965

申请日：2023-03-06

申请人： BIO-RAD LABORATORIES, INC.

发明人： Darren Roy LINK ,  John Brian HUTCHISON ,  Michael L. SAMUELS ,  Michael WEINER

IPC分类号： B01F23/41 ,  C40B50/08 ,  G01N33/50 ,  C40B40/04 ,  B01J19/00 ,  B01F25/433 ,  G01N15/14 ,  B01L3/00 ,  B01F33/3011 ,  B01L7/00 ,  B01F101/23 ,  G01N15/10

CPC分类号： B01F23/41 ,  C40B50/08 ,  G01N33/5008 ,  C40B40/04 ,  B01J19/0046 ,  B01F25/433 ,  G01N15/1459 ,  B01L3/502761 ,  B01F33/3011 ,  B01F25/4336 ,  B01L2400/0487 ,  B01L2300/0654 ,  B01J2219/00286 ,  B01J2219/00479 ,  B01L7/52 ,  B01L2300/0636 ,  B01J2219/0059 ,  B01J2219/00702 ,  B01L2200/027 ,  B01J2219/00599 ,  B01F2101/23 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00592 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01L2300/0864 ,  B01L2400/0415 ,  G01N2500/00 ,  B01J2219/00743 ,  B01J2219/0074 ,  B01L2300/0645 ,  B01J2219/00657 ,  B01J2219/00418 ,  B01J2219/00351 ,  B01L2300/0867 ,  B01J2219/0065 ,  B01L2300/0681 ,  G01N2015/1006 ,  B01J2219/00664 ,  Y02A90/10

摘要： The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US20180355350A1

公开(公告)日：2018-12-13

申请号：US15996222

申请日：2018-06-01

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/686 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6804 ,  B01L7/00 ,  B01F3/08

CPC分类号： C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US20180273935A1

公开(公告)日：2018-09-27

申请号：US15765420

申请日：2016-06-02

申请人： The Regents of the University of California

发明人： Andrew B. Lane ,  Rebecca Heald

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/113 ,  C12N15/52 ,  C12P19/34 ,  C12N15/11 ,  C12Q1/6855 ,  C40B50/08 ,  C40B40/06

CPC分类号： C12N15/1093 ,  C12N9/22 ,  C12N15/09 ,  C12N15/111 ,  C12N15/113 ,  C12N15/52 ,  C12N2310/20 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6855 ,  C12Q2521/301 ,  C12Q2521/313 ,  C12Q2525/191 ,  C40B40/06 ,  C40B50/08

摘要： The present disclosure provides methods, kits, and compositions for generating DNA molecules encoding CRISPR/Cas guide RNAs (e.g., Cas9 single guide RNAs or Cas9 targeter RNAs). A library of such DNA molecules can be generated from any DNA source. The methods include a step of contacting target DNA with one or more DNA endonucleases that specifically bind to and cleave within a recognition sequence that includes a PAM sequence, to generate a plurality of cleavage fragments, to which a DNA adapter can be attached. A distal-cleaving DNA endonuclease can be used that specifically binds to a recognition sequence in the DNA adapter and cleaves at a site within the attached DNA cleavage fragments to generate a library of CRISPR/Cas guide sequences. After removal of all or a portion of the DNA adapter, a constant region of a guide RNA can be attached to generate DNA molecules encoding CRISPR/Cas guide RNAs.

公开(公告)号：US20180272299A1

公开(公告)日：2018-09-27

申请号：US15922292

申请日：2018-03-15

申请人： President and Fellows of Harvard College ,  Medical Research Council

发明人： Andrew David Griffiths ,  David A. Weitz ,  Darren Roy Link ,  Keunho Ahn ,  Jerome Bibette

IPC分类号： B01F13/00 ,  B01F5/06 ,  B01J19/00 ,  B01L3/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K1/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  B01F3/08 ,  G01N35/10

CPC分类号： C40B40/08 ,  B01F3/0807 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0655 ,  B01F13/0071 ,  B01F13/0076 ,  B01J13/025 ,  B01J19/0046 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00599 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00889 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y10/00 ,  B82Y30/00 ,  C07K1/047 ,  C12N15/1096 ,  C12Q2600/16 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  G01N2035/1034 ,  H05K999/99

摘要： The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.

公开(公告)号：US10052605B2

公开(公告)日：2018-08-21

申请号：US12770006

申请日：2010-04-29

申请人： Andrew David Griffiths ,  Chris Abell ,  Florian Hollfelder ,  Enrico Mastrobattista

发明人： Andrew David Griffiths ,  Chris Abell ,  Florian Hollfelder ,  Enrico Mastrobattista

IPC分类号： C40B50/04 ,  C40B50/00 ,  B01J19/00 ,  B82Y30/00 ,  C07K1/04 ,  C12N15/10 ,  C40B20/04 ,  C40B30/04 ,  C40B50/08 ,  G01N33/543 ,  B82Y5/00 ,  B82Y10/00 ,  C40B40/06 ,  C40B40/10

CPC分类号： B01J19/0046 ,  B01J19/0093 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00599 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01J2219/00835 ,  B01J2219/0086 ,  B01J2219/00894 ,  B01J2219/00903 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K1/047 ,  C12N15/1075 ,  C40B20/04 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  G01N33/5432

摘要： Methods for use in the synthesis and identification of molecules which bind to a target component of a biological system or modulate the activity of a target are described.

公开(公告)号：US20180128745A1

公开(公告)日：2018-05-10

申请号：US15855612

申请日：2017-12-27

申请人： Medical Research Council

发明人： Andrew David Griffiths ,  Chris Abell ,  Florian Hollfelder ,  Enrico Mastrobattista

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  B01J2219/00596 ,  B01J2219/00599 ,  B01J2219/00702 ,  C40B50/08 ,  G01N21/6458 ,  G01N33/5432

摘要： The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, by compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsules; and identifying the compound which binds to or modulates the activity of the target. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.

公开(公告)号：US09328344B2

公开(公告)日：2016-05-03

申请号：US13759660

申请日：2013-02-05

申请人： Raindance Technologies, Inc.

发明人： Darren Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： C12Q1/68 ,  G01N33/543 ,  B01L3/00 ,  C12N15/10 ,  B01F13/00 ,  B01J19/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/68 ,  B01F3/08 ,  B01L7/00

CPC分类号： C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

摘要翻译： 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 这样的方法可以包括通过将化合物的水溶液和独特的液体标签的水溶液乳化在微流体装置上来标记化合物文库，所述微流体装置包括整体地布置在微细加工的基底上的多个可电寻址的通道支承的流体模块， 提供了用于不混溶流体流动的通道，由此每种化合物用独特的液体标记标记，汇集标记的乳液，将标记的乳液与含有特定细胞或酶的乳液聚结，从而形成纳米反应器，筛选纳米反应器以获得所需的反应 在纳米反应器的内容物之间，并解码液体标签，从而从化合物文库鉴定单一化合物。