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公开(公告)号：US20110252505A1
公开(公告)日：2011-10-13
申请号：US12897489
申请日：2010-10-04
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/113 , C12N5/10
CPC分类号： C12N15/8249 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Methods for modulating plants using optimized ACC synthase down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency and/or reduction in ethylene production.
摘要翻译： 公开了使用优化的ACC合酶下调构建体来调节植物的方法。 还公开了核苷酸序列，构建体，载体和修饰的植物细胞，以及显示增加的种子和/或生物量产量的转基因植物，对非生物胁迫如干旱或高植物密度的改善的耐受性，改善的氮利用效率和/或还原 在乙烯生产。
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公开(公告)号：US20110036002A1
公开(公告)日：2011-02-17
申请号：US12852598
申请日：2010-08-09
申请人： Jeffrey E. Habben
发明人： Jeffrey E. Habben
CPC分类号： A01G2/00
摘要： An increase in the total grain and biomass yields and photosynthetic rates of plants grown at high densities is achieved by novel methods of defoliating the primary-source leaf of a majority of such plants. In particular the methods provide for increased grain and biomass yields, including for example, increased average kernel mass for maize plants, and photosynthetic rates through the timely removal of the primary-source leaf of a majority of the plants grown at high densities. This invention also relates to seeds obtained by using the disclosed methods.
摘要翻译： 通过对大多数这种植物的主要来源叶进行脱叶的新方法，实现了以高密度生长的植物的总谷物和生物量产量和光合速率的增加。 特别地，这些方法提供了增加的谷物和生物量产量，包括例如玉米植物的平均籽粒质量增加，以及通过及时去除高密度生长的大多数植物的主要来源叶的光合速率。 本发明还涉及通过使用所公开的方法获得的种子。
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3.发明授权Maize chloroplast protein synthesis elongation factors and methods of use for same 失效玉米叶绿体蛋白合成延伸因子及其使用方法相同公开(公告)号：US07388125B2
公开(公告)日：2008-06-17
申请号：US11005896
申请日：2004-12-07
CPC分类号： C12N9/0008 , C07K14/415 , C12N15/8273
摘要： The invention discloses a novel heat shock protein with high homology to chloroplast elongation factor EF-Tu. Also disclosed is a transgenic method for enhancing tolerance to heat and drought in female reproductive organs. It involves the temporal and spatial expression of novel heat shock EF-Tu in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase heat and drought tolerance.
摘要翻译： 本发明公开了一种与叶绿体延长因子EF-Tu具有高同源性的新型热休克蛋白。 还公开了用于增强女性生殖器官耐热和干旱耐受性的转基因方法。 它涉及新型热休克EF-Tu在植物器官或植物组织中的时空表达。 本发明还包括用于生产具有可遗传表型的作物植物的表达构建体和方法，其可用于增加热量和耐旱性的育种程序。
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4.发明授权Methods and compositions for enhanced yield by targeted expression of knotted1 失效通过目标表达敲打提高产量的方法和组合物1公开(公告)号：US08431775B2
公开(公告)日：2013-04-30
申请号：US12631349
申请日：2009-12-04
CPC分类号： C12N15/8261 , C12N15/827 , C12N15/8271 , Y02A40/146
摘要： Methods and compositions for modulating expression of knotted1 (kn1) polypeptides in seed and reproductive tissue are provided. Polynucleotide sequences encoding knotted1 transcription factor polypeptides are expressed in a spatially and/or temporally regulated manner with expression preferentially in developing flowers, pods, ears and/or seeds, or to other reproductive structures during flowering and seed development, to create a sequestered effect resulting in plants that produce increased yield and may have greater flower, pod, ear and/or seed retention. Transformed plants, plant cells, tissues and seed are also provided.
摘要翻译： 提供了调节种子和生殖组织中knotted1（kn1）多肽表达的方法和组合物。 编码结1转录因子多肽的多核苷酸序列以空间和/或时间调控的方式表达，优先在开发花，荚果，穗和/或种子中表达，或者在开花和种子发育期间以其他生殖结构表达，以产生隔离效应，从而产生 在产量增加的植物中，可能具有更大的花，荚，穗和/或种子保留。 还提供了转化的植物，植物细胞，组织和种子。
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5.发明授权公开(公告)号：US08026411B2
公开(公告)日：2011-09-27
申请号：US12622891
申请日：2009-11-20
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8245 , Y02A40/146
摘要： The invention discloses a transgenic method for enhancing sink strength in female reproductive organs. It involves the temporal and spatial expression of genes affecting the starch and sucrose metabolic pathways in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase yield potential over a range of environmental conditions.
摘要翻译： 本发明公开了一种提高女性生殖器官的吸收强度的转基因方法。 它涉及影响植物器官或植物组织中淀粉和蔗糖代谢途径的基因的时间和空间表达。 本发明还包括用于生产具有可遗传表型的作物植物的表达构建体和方法，其可用于在一系列环境条件下增加产量潜力的育种程序中。
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6.发明申请公开(公告)号：US20100281578A1
公开(公告)日：2010-11-04
申请号：US12773070
申请日：2010-05-04
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots and for increasing yield in a plant are provided. The compositions include an AP2 transcription factor sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1-11 as well as variants and fragments thereof. Nucleotide sequences encoding the AP2 transcription factors are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of an AP2 transcription factor sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising an AP2 transcription factor sequence of the invention. The level of the AP2 transcription factor polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.
摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括AP2转录因子序列。 本发明的组合物包含选自SEQ ID NO：1-11的氨基酸序列和核苷酸序列以及其变体和片段。 在DNA构建体中提供了编码AP2转录因子的核苷酸序列，以提供用于在植物中表达的植物中的表达的植物或植物部分中的AP2转录因子序列的水平。 所述方法包括向植物或植物部分引入包含本发明的AP2转录因子序列的异源多核苷酸。 可以增加或减少AP2转录因子多肽的水平。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。
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公开(公告)号：US08250807B2
公开(公告)日：2012-08-28
申请号：US12852598
申请日：2010-08-09
申请人： Jeffrey E. Habben
发明人： Jeffrey E. Habben
IPC分类号： A01G1/00
CPC分类号： A01G2/00
摘要： An increase in the total grain and biomass yields and photosynthetic rates of plants grown at high densities achieved by novel methods of defoliating the primary-source leaf of a majority of such plants. In particular the methods provide for increased grain and biomass yields, including for example, increased average kernel mass for maize plants, and photosynthetic rates through the timely removal of the primary-source leaf of a majority of the plants grown at high densities. This invention also relates to seeds obtained by using the disclosed methods.
摘要翻译： 通过对大多数这样的植物的主要来源叶进行脱叶的新方法，在高密度下生长的植物的总谷物和生物量产量和光合速率的增加。 特别地，这些方法提供了增加的谷物和生物量产量，包括例如玉米植物的平均籽粒质量增加，以及通过及时去除高密度生长的大多数植物的主要来源叶的光合速率。 本发明还涉及通过使用所公开的方法获得的种子。
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8.发明申请Methods and Compositions for Enhanced Yield by Targeted Expression of Knotted1 失效通过目标表达敲打提高产量的方法和组成1公开(公告)号：US20110078822A1
公开(公告)日：2011-03-31
申请号：US12631349
申请日：2009-12-04
CPC分类号： C12N15/8261 , C12N15/827 , C12N15/8271 , Y02A40/146
摘要： Methods and compositions for modulating expression of knotted1 (kn1) polypeptides in seed and reproductive tissue are provided. Polynucleotide sequences encoding knotted1 transcription factor polypeptides are expressed in a spatially and/or temporally regulated manner with expression preferentially in developing flowers, pods, ears and/or seeds, or to other reproductive structures during flowering and seed development, to create a sequestered effect resulting in plants that produce increased yield and may have greater flower, pod, ear and/or seed retention. Transformed plants, plant cells, tissues and seed are also provided.
摘要翻译： 提供了调节种子和生殖组织中knotted1（kn1）多肽表达的方法和组合物。 编码结1转录因子多肽的多核苷酸序列以空间和/或时间调控的方式表达，优先在开发花，荚果，穗和/或种子中表达，或者在开花和种子发育期间以其他生殖结构表达，以产生隔离效应，从而产生 在产量增加的植物中，可能具有更大的花，荚，穗和/或种子保留。 还提供了转化的植物，植物细胞，组织和种子。
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公开(公告)号：US20080242545A1
公开(公告)日：2008-10-02
申请号：US11782009
申请日：2007-07-24
申请人： Jeffrey E. Habben , Shuping Jiao
发明人： Jeffrey E. Habben , Shuping Jiao
CPC分类号： C07K14/415 , C12N15/8237 , C12N15/8238
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise a novel nucleic acid sequence for a promoter which is induced by abiotic stress and the coding sequence natively associated with said promoter sequence. A method for expressing a heterologous nucleotide sequence in a plant using said promoter sequence is also provided. The methods comprise transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from said transformed plant cell.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物包括由非生物胁迫诱导的启动子的新核酸序列和与所述启动子序列本身相关的编码序列。 还提供了使用所述启动子序列在植物中表达异源核苷酸序列的方法。 所述方法包括将植物细胞转化为含有与本发明的启动子可操作地连接的异源核苷酸序列，并从所述转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US07265219B2
公开(公告)日：2007-09-04
申请号：US10702812
申请日：2003-11-06
申请人： Jeffrey E. Habben , Shuping Jiao
发明人： Jeffrey E. Habben , Shuping Jiao
CPC分类号： C07K14/415 , C12N15/8237 , C12N15/8238
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise a novel nucleic acid sequence for a promoter which is induced by abiotic stress and the coding sequence natively associated with said promoter sequence. A method for expressing a heterologous nucleotide sequence in a plant using said promoter sequence is also provided. The methods comprise transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from said transformed plant cell.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物包括由非生物胁迫诱导的启动子的新核酸序列和与所述启动子序列本身相关的编码序列。 还提供了使用所述启动子序列在植物中表达异源核苷酸序列的方法。 所述方法包括将植物细胞转化为含有与本发明的启动子可操作地连接的异源核苷酸序列，并从所述转化的植物细胞再生稳定转化的植物。
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