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公开(公告)号：US06638760B1
公开(公告)日：2003-10-28
申请号：US09204865
申请日：1998-12-03
申请人： Jer-Kang Chen , Claudia Chiesa , George A. Fry , Vergine C. Furniss , Stephen M. Lambert , Roger O'Neill , Majid Mehrpouyan
发明人： Jer-Kang Chen , Claudia Chiesa , George A. Fry , Vergine C. Furniss , Stephen M. Lambert , Roger O'Neill , Majid Mehrpouyan
IPC分类号： C12M134
CPC分类号： C12Q1/6834 , C12Q1/6837 , C12Q2565/629 , C12Q2565/507 , C12Q2525/197
摘要： The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.
摘要翻译： 本发明提供了用于核酸的有效，快速和特异性捕获以及最佳回收的底物和装置，以及其使用方法。 底物本质上是多孔的，并且具有能够固定在其上的靶核酸杂交的捕获多核苷酸。 当含有或怀疑含有靶核酸的样品通过多孔底物流动时，靶核酸被快速捕获。 捕获后，靶核酸可以有效地回收以备后续使用。
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2.发明授权Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers 有权将金属氧化物沉积在表面上，作为羧化或磷化颗粒或聚合物的固定载体公开(公告)号：US08173198B2
公开(公告)日：2012-05-08
申请号：US12508539
申请日：2009-07-23
CPC分类号： C07F9/6596 , C07F9/093 , C12Q1/6834 , Y10S530/81
摘要： Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y. The metal oxide surfaces exhibit minimal binding to bio-molecules, exhibit uniform deposition and immobilization of beads at high density, can be subsequently modified to create surfaces having a variety of properties, and can be used for nucleic acid sequencing and other analyses and in single-molecule detection schemes.
摘要翻译： 描述了形成与氧化物层上的表面结合的活化金属配合物的中间体和方法，将珠子固定在改性表面上以及由此制备的制品。 氧化物表面上的羟基与式Y（L-Pol）m的金属试剂络合物反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol” 代表钝化剂如甲氧基乙醇，聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸盐官能团或与Y形成复合物的多个官能团的钝化剂反应。金属氧化物表面表现出与生物分子的最小结合，表现出均匀的沉积和 可以随后修饰珠子以高密度的固定，以产生具有多种性质的表面，并可用于核酸测序和其他分析以及单分子检测方案。
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3.发明申请DEPOSITION OF METAL OXIDES ONTO SURFACES AS AN IMMOBILIZATION VEHICLE FOR CARBOXYLATED OR PHOPHATED PARTICLES OR POLYMERS 有权将金属氧化物沉积在表面上作为用于羧基化或亲水化的颗粒或聚合物的固定化载体公开(公告)号：US20100086927A1
公开(公告)日：2010-04-08
申请号：US12508539
申请日：2009-07-23
CPC分类号： C07F9/6596 , C07F9/093 , C12Q1/6834 , Y10S530/81
摘要： Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y. The metal oxide surfaces exhibit minimal binding to bio-molecules, exhibit uniform deposition and immobilization of beads at high density, can be subsequently modified to create surfaces having a variety of properties, and can be used for nucleic acid sequencing and other analyses and in single-molecule detection schemes.
摘要翻译： 描述了形成与氧化物层上的表面结合的活化金属配合物的中间体和方法，将珠子固定在改性表面上以及由此制备的制品。 氧化物表面上的羟基与式Y（L-Pol）m的金属试剂络合物反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol” 代表钝化剂如甲氧基乙醇，聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸盐官能团或与Y形成复合物的多个官能团的钝化剂反应。金属氧化物表面表现出与生物分子的最小结合，表现出均匀的沉积， 可以随后修饰珠子以高密度的固定，以产生具有多种性质的表面，并可用于核酸测序和其他分析以及单分子检测方案。
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