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22 条记录 (耗时 0.035 秒)

    Methods and kits for methylation detection
    1.
    发明授权
    Methods and kits for methylation detection 有权
    用于甲基化检测的方法和试剂盒

    公开(公告)号：US07364855B2

    公开(公告)日：2008-04-29

    申请号：US11119985

    申请日：2005-05-02

    申请人： Mark R. Andersen ,  Jer-Kang Chen ,  Michael W. Hunkapiller ,  Steven M. Menchen

    发明人： Mark R. Andersen ,  Jer-Kang Chen ,  Michael W. Hunkapiller ,  Steven M. Menchen

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q2537/164 ,  C12Q2521/501

    摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.

    摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。

    Methods and Kits for Methylation Detection
    2.
    发明申请
    Methods and Kits for Methylation Detection 审中-公开
    甲基化检测方法和试剂盒

    公开(公告)号：US20110143343A1

    公开(公告)日：2011-06-16

    申请号：US12843877

    申请日：2010-07-26

    申请人： MARK R. ANDERSEN ,  JER-KANG CHEN ,  MICHAEL W. HUNKAPILLER ,  STEVEN M. MENCHEN

    发明人： MARK R. ANDERSEN ,  JER-KANG CHEN ,  MICHAEL W. HUNKAPILLER ,  STEVEN M. MENCHEN

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q2537/164 ,  C12Q2521/501

    摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.

    摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。

    Multiplex polynucleotide capture methods and compositions
    5.
    发明授权
    Multiplex polynucleotide capture methods and compositions 有权

    公开(公告)号：US06514699B1

    公开(公告)日：2003-02-04

    申请号：US09593312

    申请日：2000-06-13

    申请人： Roger A. O'Neill ,  Jer-Kang Chen ,  Claudia Chiesa ,  George Fry

    发明人： Roger A. O'Neill ,  Jer-Kang Chen ,  Claudia Chiesa ,  George Fry

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6874 ,  C12Q2565/514 ,  C12Q2537/157 ,  C12Q2525/161 ,  C12Q2565/629

    摘要： The invention relates to methods and compositions for simultaneously generating a plurality of polynucleotide sequencing ladders or PCR amplification products. Each sequencing ladder is generated from a recoverable primer, i.e., an oligonucleotide primer comprising a recovery tag. The recovery tag may be an oligonucleotide. Each sequencing ladder has a unique recovery tag. After the generation of the multiple sequencing ladders, the different sequencing ladders are separated from one another, i.e., purified, by binding to recovery tag binding compounds that have been immobilized on one or more solid supports. The recovery tag binding compounds are immobilized on the solid support in an addressable manner, i.e., the recovery tag binding compounds have distinct locations on the solid support. The binding of the sequencing ladders to the recovery tag binding compounds serves to separate the different polynucleotide sequencing ladders present in a given solution. The separated sequencing ladders may then be released from the immobilized recovery tag binding compounds and subsequently resolved into individual components of the sequencing ladders or PCR products. The subject methods of separating sequencing ladders simultaneously generated in the same vessel may readily be adapted to separate a plurality of simultaneously generated polynucleotide amplification products. Other aspects of the invention are kits for the generation or recovery of a plurality of polynucleotide sequencing ladders or amplification products. The kits comprise a plurality of recoverable primers having unique recovery tags. Preferably, the recovery tags are polynucleotides that have substantially the same denaturation temperature when bound to appropriate recovery tag binding compounds, i.e., the primers comprise an integrated set. The kits may further comprise recovery tag binding compounds. The kits may further comprise labeled chain terminators. Other aspects of the invention include polynucleotide recovery devices.

    Oligonucleotide N3′→N5′Phosphoramidate Duplexes
    6.
    发明授权
    Oligonucleotide N3′→N5′Phosphoramidate Duplexes 失效
    寡核苷酸N 3' - > N 5'磷酰胺复合体

    公开(公告)号：US06169170A

    公开(公告)日：2001-01-02

    申请号：US08923386

    申请日：1997-09-03

    申请人： Sergei M. Gryaznov ,  Ronald G. Schultz ,  Jer-kang Chen

    发明人： Sergei M. Gryaznov ,  Ronald G. Schultz ,  Jer-kang Chen

    IPC分类号： C07H2100

    CPC分类号： C07H19/06 ,  C07H19/16 ,  C07H21/00

    摘要： Modified oligonucleotides 3′-NHP(O)(O−)O-5′ phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.

    摘要翻译： 在固相载体上合成修饰的寡核苷酸3'-NHP（O）（O）O-5'氨基磷酸酯。 发现氨基磷酸酯类似物对磷酸二酯酶消化具有显着增加的抗性。 热解离实验表明，这些化合物比具有互补DNA特别是RNA链的磷酸二酯形成更稳定的双链体。 此外，氨基磷酸酯类似物还可以形成具有双链DNA靶的稳定的三链体，其中在类似条件下，亲代磷酸二酯化合物不能这样做。

    Methods and kits for methylation detection
    9.
    发明申请
    Methods and kits for methylation detection 有权
    用于甲基化检测的方法和试剂盒

    公开(公告)号：US20050266458A1

    公开(公告)日：2005-12-01

    申请号：US11119985

    申请日：2005-05-02

    申请人： Mark Andersen ,  Jer-Kang Chen ,  Michael Hunkapiller ,  Steven Menchen

    发明人： Mark Andersen ,  Jer-Kang Chen ,  Michael Hunkapiller ,  Steven Menchen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q2537/164 ,  C12Q2521/501

    摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.

    摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。

    Multiplex polynucleotide capture methods and compositions
    10.
    发明授权
    Multiplex polynucleotide capture methods and compositions 失效

    公开(公告)号：US6124092A

    公开(公告)日：2000-09-26

    申请号：US873437

    申请日：1997-06-12

    申请人： Roger A. O'Neill ,  Jer-Kang Chen ,  Claudia Chiesa ,  George Fry

    发明人： Roger A. O'Neill ,  Jer-Kang Chen ,  Claudia Chiesa ,  George Fry

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6874

    摘要： The invention relates to methods and compositions for simultaneously generating a plurality of polynucleotide sequencing ladders or PCR amplification products. Each sequencing ladder is generated from a recoverable primer, i.e., an oligonucleotide primer comprising a recovery tag. The recovery tag may be an oligonucleotide. Each sequencing ladder has a unique recovery tag. After the generation of the multiple sequencing ladders, the different sequencing ladders are separated from one another, i.e., purified, by binding to recovery tag binding compounds that have been immobilized on one or more solid supports. The recovery tag binding compounds are immobilized on the solid support in an addressable manner, i.e., the recovery tag binding compounds have distinct locations on the solid support. The binding of the sequencing ladders to the recovery tag binding compounds serves to separate the different polynucleotide sequencing ladders present in a given solution. The separated sequencing ladders may then be released from the immobilized recovery tag binding compounds and subsequently resolved into individual components of the sequencing ladders or PCR products. The subject methods of separating sequencing ladders simultaneously generated in the same vessel may readily be adapted to separate a plurality of simultaneously generated polynucleotide amplification products. Other aspects of the invention are kits for the generation or recovery of a plurality of polynucleotide sequencing ladders or amplification products. The kits comprise a plurality of recoverable primers having unique recovery tags. Preferably, the recovery tags are polynucleotides that have substantially the same denaturation temperature when bound to appropriate recovery tag binding compounds, i.e., the primers comprise an integrated set. The kits may further comprise recovery tag binding compounds. The kits may further comprise labeled chain terminators. Other aspects of the invention include polynucleotide recovery devices.