Methods for transforming immature maize embryos

    公开(公告)号:US07057089B2

    公开(公告)日:2006-06-06

    申请号:US09993080

    申请日:2001-11-13

    IPC分类号: C12N15/05 C12N15/82 C12N15/90

    CPC分类号: A01H4/001

    摘要: Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.