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1.发明授权Cell for preparing competent cell, method for preparing competent cell and bacterial strain of Escherichia coli 有权用于制备感受态细胞的细胞，用于制备感受态细胞和大肠杆菌细菌菌株的方法公开(公告)号：US08512996B2
公开(公告)日：2013-08-20
申请号：US12979079
申请日：2010-12-27
申请人： Jia-Hung Wang , Meng-Yin Tsai , Ting-Ting Hung , Kelly Teng
发明人： Jia-Hung Wang , Meng-Yin Tsai , Ting-Ting Hung , Kelly Teng
CPC分类号： C12Y301/03012 , C12N9/16
摘要： The invention provides a cell for preparing competent cells, wherein the cell is capable of spontaneously accumulating self-producing trehalose therein and the cell is used for the preparation of competent cells. The invention also provides a method for preparing competent cells, including: culturing the cell for preparing the competent cell mentioned previously to obtain a cell suspension; placing the cell suspension into an ice bath; centrifuging the cell suspension to obtain a cell precipitate; mixing a transform reagent with the cell precipitate; and obtaining competent cells suspension.
摘要翻译： 本发明提供了一种用于制备感受态细胞的细胞，其中细胞能够自发地在其中积累自产海藻糖，并且该细胞用于制备感受态细胞。 本发明还提供一种制备感受态细胞的方法，包括：培养用于制备前述感受态细胞的细胞以获得细胞悬浮液; 将细胞悬浮液置于冰浴中; 离心细胞悬浮液以获得细胞沉淀; 将转化试剂与细胞沉淀物混合; 并获得感受态细胞悬浮液。
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公开(公告)号：US08192965B2
公开(公告)日：2012-06-05
申请号：US12546834
申请日：2009-08-25
申请人： Jia-Hung Wang , Shu-Hsien Tsai , Kelly Teng
发明人： Jia-Hung Wang , Shu-Hsien Tsai , Kelly Teng
摘要： Method for producing itaconic acid in yeast cells using glycerol as the substrate. The yeast cells express cis-aconitic acid decarboxylase and optionally, citrate synthase and/or aconitase at high levels.
摘要翻译： 使用甘油作为底物在酵母细胞中生产衣康酸的方法。 酵母细胞以高水平表达顺式乌头酸脱羧酶和任选的柠檬酸合酶和/或乌头酸酶。
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公开(公告)号：US20110053232A1
公开(公告)日：2011-03-03
申请号：US12546834
申请日：2009-08-25
申请人： Jia-Hung Wang , Shu-Hsien Tsai , Kelly Teng
发明人： Jia-Hung Wang , Shu-Hsien Tsai , Kelly Teng
IPC分类号： C12P7/44
摘要： Method for producing itaconic acid in yeast cells using glycerol as the substrate. The yeast cells express cis-aconitic acid decarboxylase and optionally, citrate synthase and/or aconitase at high levels.
摘要翻译： 使用甘油作为底物在酵母细胞中生产衣康酸的方法。 酵母细胞以高水平表达顺式乌头酸脱羧酶和任选的柠檬酸合酶和/或乌头酸酶。
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4.发明申请HARD DRIVE ACCESSING METHOD AND HARD DRIVE ACCESSING SYSTEM SUPPORTING MAXIMUM TRANSMISSION RATE OF HARD DRIVE 有权硬盘驱动器访问方法和硬盘驱动器访问系统支持硬盘驱动器的最大传输速率公开(公告)号：US20100205365A1
公开(公告)日：2010-08-12
申请号：US12416605
申请日：2009-04-01
申请人： Chung-Ching Huang , Chin-Han Chang , Jia-Hung Wang
发明人： Chung-Ching Huang , Chin-Han Chang , Jia-Hung Wang
IPC分类号： G06F13/00
CPC分类号： G06F13/385
摘要： A hard drive assessing method and a hard drive assessing system supporting a maximum transmission rate of a hard drive are provided, wherein the hard drive is accessed by a controller, and both the controller and the hard drive support a plurality of transmission rates. The maximum transmission rate of the hard drive is first obtained. When the controller reads data from the hard drive, the transmission rate of the controller is set to be not lower than the maximum transmission rate, and the transmission rate of the hard drive is maintained at the maximum transmission rate. When the controller writes data into the hard drive, the transmission rate of the controller is reduced to be lower than the maximum transmission rate, and the transmission rate of the hard drive is maintained at the maximum transmission rate. Thereby, the hard drive can be accessed at its maximum transmission rate.
摘要翻译： 提供了硬盘驱动器评估方法和支持硬盘驱动器的最大传输速率的硬盘驱动器评估系统，其中硬盘驱动器被控制器访问，并且控制器和硬盘驱动器都支持多个传输速率。 首先获得硬盘驱动器的最大传输速率。 当控制器从硬盘驱动器读取数据时，将控制器的传输速率设置为不低于最大传输速率，并将硬盘驱动器的传输速率保持在最大传输速率。 当控制器将数据写入硬盘驱动器时，控制器的传输速率降低到低于最大传输速率，硬盘驱动器的传输速率保持在最大传输速率。 因此，可以以其最大传输速率访问硬盘驱动器。
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5.发明授权Hard drive accessing method and hard drive accessing system supporting maximum transmission rate of hard drive 有权支持硬盘驱动器最大传输速率的硬盘访问方式和硬盘访问系统公开(公告)号：US08108598B2
公开(公告)日：2012-01-31
申请号：US12416605
申请日：2009-04-01
申请人： Chung-Ching Huang , Chin-Han Chang , Jia-Hung Wang
发明人： Chung-Ching Huang , Chin-Han Chang , Jia-Hung Wang
IPC分类号： G06F13/00
CPC分类号： G06F13/385
摘要： A hard drive assessing method and a hard drive assessing system supporting a maximum transmission rate of a hard drive are provided, wherein the hard drive is accessed by a controller, and both the controller and the hard drive support a plurality of transmission rates. The maximum transmission rate of the hard drive is first obtained. When the controller reads data from the hard drive, the transmission rate of the controller is set to be not lower than the maximum transmission rate, and the transmission rate of the hard drive is maintained at the maximum transmission rate. When the controller writes data into the hard drive, the transmission rate of the controller is reduced to be lower than the maximum transmission rate, and the transmission rate of the hard drive is maintained at the maximum transmission rate. Thereby, the hard drive can be accessed at its maximum transmission rate.
摘要翻译： 提供了硬盘驱动器评估方法和支持硬盘驱动器的最大传输速率的硬盘驱动器评估系统，其中硬盘驱动器被控制器访问，并且控制器和硬盘驱动器都支持多个传输速率。 首先获得硬盘驱动器的最大传输速率。 当控制器从硬盘驱动器读取数据时，将控制器的传输速率设置为不低于最大传输速率，并将硬盘驱动器的传输速率保持在最大传输速率。 当控制器将数据写入硬盘驱动器时，控制器的传输速率降低到低于最大传输速率，硬盘驱动器的传输速率保持在最大传输速率。 因此，可以以其最大传输速率访问硬盘驱动器。
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