公开(公告)号：US08722364B2

公开(公告)日：2014-05-13

申请号：US13228572

申请日：2011-09-09

申请人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

发明人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

IPC分类号： C12P21/04

CPC分类号： C12N9/0065 ,  C12N1/20

摘要： Disclosed are methods for increasing microbial catalase production. 1-10 g/L sodium hexametaphosphate was added to the culture medium between 30-40 hours of fermentation to inhibit proteinase activity and increase the production of catalase. This simple modification of fermentation procedure can result in up to 45% increase of the production of catalase.

摘要翻译： 公开了增加微生物过氧化氢酶产生的方法。 在发酵30-40小时之间向培养基中加入1-10g / L六偏磷酸钠以抑制蛋白酶活性并增加过氧化氢酶的产生。 发酵过程的这种简单的修改可以导致过氧化氢酶的产量提高高达45％。

公开(公告)号：US20130065292A1

公开(公告)日：2013-03-14

申请号：US13228572

申请日：2011-09-09

申请人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

发明人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

IPC分类号： C12N9/08 ,  C12N1/20

CPC分类号： C12N9/0065 ,  C12N1/20

摘要： Disclosed are methods for increasing microbial catalase production. 1-10 g/L sodium hexametaphosphate was added to the culture medium between 30-40 hours of fermentation to inhibit proteinase activity and increase the production of catalase. This simple modification of fermentation procedure can result in up to 45% increase of the production of catalase.

摘要翻译： 公开了增加微生物过氧化氢酶产生的方法。 在发酵30-40小时之间向培养基中加入1-10g / L六偏磷酸钠以抑制蛋白酶活性并增加过氧化氢酶的产生。 发酵过程的这种简单的修改可以导致过氧化氢酶的产量提高高达45％。

公开(公告)号：US20170009267A1

公开(公告)日：2017-01-12

申请号：US14934166

申请日：2015-11-06

申请人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

发明人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

IPC分类号： C12P19/26 ,  C12N15/75

CPC分类号： C12P19/26 ,  C12N15/75 ,  C12R1/125

摘要： The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h−1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.

摘要翻译： 本发明提供了通过使用具有glcK敲除的重组枯草芽孢杆菌来增强N-乙酰葡糖胺产生的方法。 本发明通过敲除编码葡糖激酶的glcK基因来增强GlcNAc的产生，从而消除GlcNAc磷酸化成GlcNAc-6-P。 具有glcK敲除的重组枯草芽孢杆菌上清液中GlcNAc的比生长速率和含量分别为0.15h-1和3.0g / L，分别为没有glcK敲除的对照菌株的2.32倍和2.14倍。 本发明的重组枯草芽孢杆菌可能用于GlcNAc的工业生产。

公开(公告)号：US09187773B2

公开(公告)日：2015-11-17

申请号：US14047968

申请日：2013-10-07

申请人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

发明人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

IPC分类号： C12P7/52

CPC分类号： C12P7/52

摘要： The invention provides a simple and effective method for improving acid tolerance of P. acidipropionici by adding arginine and/or aspartic acid to the culture medium. The acid tolerance of P. acidipropionici was improved by 60% and 20% respectively through adding arginine or aspartic acid into the culture medium. Consequently, PA production was improved by 36% and 26%, respectively. The maximal PA production was obtained by adding both 20 mM arginine and 20 mM aspartic acid. This method can be applied to large scale production of PA.

摘要翻译： 本发明提供了一种通过向培养基中加入精氨酸和/或天冬氨酸来改善酸性丙酸酸的耐酸性的简单和有效的方法。 通过向培养基中加入精氨酸或天冬氨酸，分别将酸性丙酸的耐酸性提高了60％和20％。 因此，PA产量分别提高了36％和26％。 通过加入20mM精氨酸和20mM天冬氨酸获得最大的PA产生。 该方法可应用于大规模生产PA。

公开(公告)号：US20140178952A1

公开(公告)日：2014-06-26

申请号：US14047968

申请日：2013-10-07

申请人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

发明人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

IPC分类号： C12P7/52

CPC分类号： C12P7/52

摘要： The invention provides a simple and effective method for improving acid tolerance of P. acdipropionici by adding arginine and/or aspartic acid to the culture medium. The acid tolerance of P. acdipropionici was improved by 60% and 20% respectively through adding arginine or aspartic acid into the culture medium. Consequently, PA production was improved by 36% and 26%, respectively. The maximal PA production was obtained by adding both 20 mM arginine and 20 mM aspartic acid. This method can be applied to large scale production of PA.

摘要翻译： 本发明提供了一种简单有效的方法，通过向培养基中加入精氨酸和/或天冬氨酸来改善丙酸二氢吡啶的耐酸性。 通过向培养基中加入精氨酸或天冬氨酸，分别提高了丙酰丙酸的耐酸性分别提高了60％和20％。 因此，PA产量分别提高了36％和26％。 通过加入20mM精氨酸和20mM天冬氨酸获得最大的PA产生。 该方法可应用于大规模生产PA。

公开(公告)号：US09914949B2

公开(公告)日：2018-03-13

申请号：US14934166

申请日：2015-11-06

申请人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

发明人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

IPC分类号： C12N15/75 ,  C12P19/26

CPC分类号： C12P19/26 ,  C12N15/75 ,  C12R1/125

摘要： The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h−1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.