公开(公告)号：US08980563B2

公开(公告)日：2015-03-17

申请号：US12972208

申请日：2010-12-17

申请人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

发明人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2531/125

摘要： Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.

摘要翻译： 公开了从复杂混合物多个扩增不同序列的多个靶的方法。 在一个方面，使用单个环化探针对靶标进行环化，所述单个环化探针与靶区域中将要扩增的区域侧翼的两个区域互补。 目标可以与环化探针杂交，从而产生5'或3'的瓣，并且公开了用于去除襟翼和使所得产物环化的方法。 另一方面，目标与dU探针杂交，从而产生5'和3'瓣。 使用5'或3'片段内切核酸酶或3'至5'外切核酸酶切割襟翼。 然后将靶序列连接到常见引物，消化dU探针并扩增连接的靶标。

公开(公告)号：US20080199916A1

公开(公告)日：2008-08-21

申请号：US12016195

申请日：2008-01-17

申请人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

发明人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

IPC分类号： C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2531/125

摘要： Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.

摘要翻译： 公开了从复杂混合物多个扩增不同序列的多个靶的方法。 在一个方面，使用单个环化探针对靶标进行环化，所述单个环化探针与靶区域中将要扩增的区域侧翼的两个区域互补。 目标可以与环化探针杂交，从而产生5'或3'的瓣，并且公开了用于去除襟翼和使所得产物环化的方法。 另一方面，目标与dU探针杂交，从而产生5'和3'瓣。 使用5'或3'片段内切核酸酶或3'至5'外切核酸酶切割襟翼。 然后将靶序列连接到常见引物，消化dU探针并扩增连接的靶标。

公开(公告)号：US20110124052A1

公开(公告)日：2011-05-26

申请号：US12972208

申请日：2010-12-17

申请人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

发明人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

IPC分类号： C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2531/125

摘要： Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.

摘要翻译： 公开了从复杂混合物多个扩增不同序列的多个靶的方法。 在一个方面，使用单个环化探针对靶标进行环化，所述单个环化探针与靶区域中将要扩增的区域侧翼的两个区域互补。 目标可以与环化探针杂交，从而产生5'或3'的瓣，并且公开了用于去除襟翼和使所得产物环化的方法。 另一方面，目标与dU探针杂交，从而产生5'和3'瓣。 使用5'或3'片段内切核酸酶或3'至5'外切核酸酶切割襟翼。 然后将靶序列连接到常见引物，消化dU探针并扩增连接的靶标。

公开(公告)号：US07862999B2

公开(公告)日：2011-01-04

申请号：US12016195

申请日：2008-01-17

申请人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

发明人： Jianbiao Zheng ,  Li Weng ,  Malek Faham

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2531/125

摘要： Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.

摘要翻译： 公开了从复杂混合物多个扩增不同序列的多个靶的方法。 在一个方面，使用单个环化探针对靶标进行环化，所述单个环化探针与靶区域中将要扩增的区域侧翼的两个区域互补。 目标可以与环化探针杂交，从而产生5'或3'的瓣，并且公开了用于去除襟翼和使所得产物环化的方法。 另一方面，目标与dU探针杂交，从而产生5'和3'瓣。 使用5'或3'片段内切核酸酶或3'至5'外切核酸酶切割襟翼。 然后将靶序列连接到常见引物，消化dU探针并扩增连接的靶标。

公开(公告)号：US20150171770A1

公开(公告)日：2015-06-18

申请号：US14381598

申请日：2012-04-24

申请人： Robert Gregory Wagoner ,  Yong Xu ,  Li Weng ,  Hao Yu

发明人： Robert Gregory Wagoner ,  Yong Xu ,  Li Weng ,  Hao Yu

IPC分类号： H02M7/5387 ,  H02M1/08

CPC分类号： H02M7/5387 ,  H02M1/08 ,  H02M2001/0054

摘要： A power converter and a method for improving the output power of the power converter. The method includes independently selecting different gate resistor characteristics for the upper switching element and the lower switching element in the switching modules of the power converter to improve switching performance of both the upper and the lower switching element across a wide range of operating conditions, such as different voltages, currents, and/or operating temperatures.

摘要翻译： 一种功率转换器和用于提高功率转换器的输出功率的方法。 该方法包括在功率转换器的开关模块中独立地选择上开关元件和下开关元件的不同栅极电阻器特性，以改善上下开关元件在宽范围的工作条件下的开关性能，例如 不同的电压，电流和/或工作温度。

公开(公告)号：US5269297A

公开(公告)日：1993-12-14

申请号：US842529

申请日：1992-02-27

申请人： Li Weng ,  Robert M. Scribner

发明人： Li Weng ,  Robert M. Scribner

IPC分类号： A61B17/00 ,  A61B17/22 ,  A61B17/32 ,  A61B18/00 ,  B06B3/00 ,  A61H23/00

CPC分类号： B06B3/00 ,  A61B17/22012 ,  A61B2017/00336 ,  A61B2017/22018 ,  A61B2017/22092 ,  A61B2017/320072

摘要： A horn connectable to an energy source to amplify ultrasound displacement is connected to a transmitter formed of material having relatively high mechanical Q for transmitting ultrasonic energy therethrough at a frequency f, the transmitter having a horn-shaped configuration of length that is a multiple of a half-wavelength .lambda./2, and preferably this horn-shaped configuration is comprised of multiple horn segments, each of a length substantially equal to a multiple of .lambda./2, where .lambda.=c/f (c is the speed of sound in the high Q material). The transmitter has a proximal end of cross-sectional diameter D.sub.1 connected to the horn and a distal end of cross-sectional diameter D.sub.2, where D.sub.1 >D.sub.2. Ultrasonic energy transmitted through the transmitter drives a tip which is coupled to the transmitter by means of a flexible connector.

摘要翻译： 可连接到能量源以扩大超声位移的喇叭连接到由具有相对较高机械Q的材料形成的发射器，用于以频率f传输超声能量，发射器具有长度为 半波长λ/ 2，并且优选地，该喇叭形配置由多个喇叭片段组成，每个喇叭片段的长度基本上等于λ/ 2的倍数，其中λ= c / f（c是声音的速度 高Q材质）。 发射器具有连接到喇叭的截面直径D1的近端和横截面直径D2的远端，其中D1> D2。 通过变送器传输的超声能量通过柔性连接器驱动与发射机耦合的尖端。