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    USE OF MICROVESICLES IN ANALYZING KRAS MUTATIONS
    4.
    发明申请
    USE OF MICROVESICLES IN ANALYZING KRAS MUTATIONS 审中-公开
    微分子在分析KRAS突变中的应用

    公开(公告)号：US20130029339A1

    公开(公告)日：2013-01-31

    申请号：US13395354

    申请日：2010-09-09

    申请人： Johan Karl Olov Skog ,  Xandra O. Breakefield

    发明人： Johan Karl Olov Skog ,  Xandra O. Breakefield

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2527/125

    摘要： Microvesicles are small membrane vesicles that either shed or bud off eukarotic cells. Analysis of the nucleic acid content of microvesicles may be useful in detecting the presence or absence of genetic aberrations. This invention discloses novel methods of diagnosing, prognosing, monitoring, or treating a disease, such as cancer, or other medical condition in a subject involving analyzing one or more nucleic acids contained within an isolated microvesicle for the presence or absence of one or more Kras genetic aberrations.

    摘要翻译： 微泡是脱落或脱落的真核细胞的小囊泡。 微泡核酸含量的分析可用于检测遗传畸变的存在或不存在。 本发明公开了一种诊断，预后，监测或治疗疾病如癌症或其他医学病症的新方法，所述方法涉及分析一种或多种Kras存在或不存在于分离的微囊中的一种或多种核酸 遗传畸变。

    MICROVESICLE-BASED ASSAYS
    6.
    发明申请
    MICROVESICLE-BASED ASSAYS 审中-公开
    基于微波的测定

    公开(公告)号：US20140147839A1

    公开(公告)日：2014-05-29

    申请号：US13878668

    申请日：2011-10-17

    申请人： Walter Chen ,  Xandra O. Breakefield ,  Leonora Balaj ,  Johan Karl Olov Skog

    发明人： Walter Chen ,  Xandra O. Breakefield ,  Leonora Balaj ,  Johan Karl Olov Skog

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/156

    摘要： Methods are disclosed herein for assaying a biological sample or a bodily fluid obtained from a subject by isolating, obtaining or using a microvesicle fraction from the biological sample or bodily fluid and detecting in the microvesicle fraction the presence or absence of a genetic aberration in an IDH1, IDH2, TP53, PTEN, CDKN2A, NF1, EGFR, RB1, PIK3CA, or BRAF gene. The methods may be used for aiding the diagnosis, prognosis, monitoring, or therapy selection in relation to a disease or other medical condition (e.g., a glioma) in a subject.

    摘要翻译： 本文公开了用于通过分离，获得或使用来自生物样品或体液的微泡级分来检测从受试者获得的生物样品或体液的方法，并在微泡级分中检测IDH1中存在或不存在遗传差异 ，IDH2，TP53，PTEN，CDKN2A，NF1，EGFR，RB1，PIK3CA或BRAF基因。 所述方法可以用于帮助与受试者中的疾病或其他医学状况（例如胶质瘤）相关的诊断，预后，监测或治疗选择。

    Engraftable Neural Progenitor and Stem Cells for Brain Tumor Therapy
    8.
    发明申请
    Engraftable Neural Progenitor and Stem Cells for Brain Tumor Therapy 审中-公开
    可植入神经祖细胞和干细胞用于脑肿瘤治疗

    公开(公告)号：US20080152590A1

    公开(公告)日：2008-06-26

    申请号：US12044204

    申请日：2008-03-07

    申请人： Evan Y. Snyder ,  Karen S. Aboody ,  Xandra O. Breakefield ,  William P. Lynch

    发明人： Evan Y. Snyder ,  Karen S. Aboody ,  Xandra O. Breakefield ,  William P. Lynch

    IPC分类号： A61K49/00 ,  A61K35/00 ,  A61P43/00

    CPC分类号： C12N7/00 ,  A61K35/12 ,  A61K38/00 ,  A61K48/0008 ,  A61K48/0075 ,  C12N5/0623 ,  C12N15/86 ,  C12N2510/00 ,  C12N2710/16052 ,  C12N2710/16643 ,  C12N2740/10052 ,  Y02A50/473

    摘要： One of the impediments to the treatment of some human brain tumors (e.g. gliomas) has been the degree to which they expand, migrate widely, and infiltrate normal tissue. We demonstrate that a clone of multipotent neural progenitor stem cells, when implanted into an experimental glioma, will migrate along with and distribute themselves throughout the tumor in juxtaposition to widely expanding and aggressively advancing tumor cells, while continuing to express a foreign reporter gene. Furthermore, drawn somewhat by the degenerative environment created just beyond the infiltrating tumor edge, the neural progenitor cells migrate slightly beyond and surround the invading tumor border. When implanted at a distant sight from the tumor bed (e.g., into normal tissue, into the contralateral hemisphere, into the lateral ventricles) the donor neural progenitor/stem cells will migrate through normal tissue and specifically target the tumor cells. These results suggest the adjunctive use of neural progenitor/stem cells as a novel, effective delivery vehicle for helping to target therapeutic genes and vectors to invasive brain tumors that have been refractory to treatment.

    摘要翻译： 治疗某些人脑肿瘤（例如神经胶质瘤）的障碍之一是它们扩张，迁移广泛并渗透正常组织的程度。 我们证明，当植入实验性神经胶质瘤时，多潜能神经祖细胞的克隆将与整个肿瘤一起迁移并分布在整个肿瘤中，并与广泛扩张和积极进展的肿瘤细胞并列，同时继续表达外源报告基因。 此外，由于刚刚超出渗透性肿瘤边缘的退化环境，神经祖细胞迁移稍微超出并包围入侵肿瘤边界。 当植入远离肿瘤床（例如，进入正常组织，进入对侧半球，进入侧脑室）时，供体神经祖细胞/干细胞将迁移通过正常组织并且特异性靶向肿瘤细胞。 这些结果表明辅助性使用神经祖细胞/干细胞作为一种新型有效的递送载体，用于帮助将治疗基因和载体靶向治疗难治性的侵袭性脑肿瘤。

    Herpes simplex virus amplicon vector targeting system and method of using same
    10.
    发明授权
    Herpes simplex virus amplicon vector targeting system and method of using same 失效
    单纯疱疹病毒扩增子载体靶向系统及其使用方法

    公开(公告)号：US06673602B1

    公开(公告)日：2004-01-06

    申请号：US09592537

    申请日：2000-06-12

    申请人： Matthew A. Spear ,  Xandra O. Breakefield

    发明人： Matthew A. Spear ,  Xandra O. Breakefield

    IPC分类号： C12N1500

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/16643 ,  C12N2710/16645 ,  C12N2810/40 ,  C12N2810/60

    摘要： The present invention relates to herpes simplex virus (HSV) amplicon vectors, and in particular, HSV-1 amplicon vectors, which have been genetically modified and used alone or with consequent genetically modified HSV virus, to target a selected cell type, such as neoplastic cells. The present invention also relates to methods of using such vectors to target a cell, in order to treat a pathologic condition, such as cancer.

    摘要翻译： 本发明涉及单纯疱疹病毒（HSV）扩增子载体，特别是HSV-1扩增子载体，其已被遗传修饰并且单独使用或随后使用遗传修饰的HSV病毒靶向所选择的细胞类型，例如肿瘤 细胞。 本发明还涉及使用这些载体靶向细胞的方法，以便治疗诸如癌症的病理状况。