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    Composition and method for stabilizing fluorescent particles
    2.
    发明授权
    Composition and method for stabilizing fluorescent particles 有权
    用于稳定荧光颗粒的组合物和方法

    公开(公告)号：US08877505B2

    公开(公告)日：2014-11-04

    申请号：US13382509

    申请日：2011-02-01

    申请人： Eric May ,  Alexandra Nagy ,  Jerome Kosmeder

    发明人： Eric May ,  Alexandra Nagy ,  Jerome Kosmeder

    IPC分类号： G01N21/64 ,  C12Q1/68 ,  G01N33/533 ,  C09K11/02 ,  G01N33/58 ,  B82Y15/00

    CPC分类号： C09K11/025 ,  B82Y15/00 ,  C09K11/883 ,  G01N33/533 ,  G01N33/588 ,  Y10S977/774 ,  Y10S977/902 ,  Y10T436/108331

    摘要： Embodiments of a composition for stabilizing fluorescent signal of nanoparticles and methods for its use are disclosed. In some embodiments, the composition has a pH from 7 to 10 and includes borate, protein and/or protein hydrolysate, an amine, a preservative, and a nonionic surfactant. In particular embodiments, the amine is an N-ethanol substituted amine, such as ethanolamine, diethanolamine, triethanolamine, N-methyldiethanolamine, N,N-dimethylethanolamine, or a combination thereof. In some embodiments, a fluorescent particle solution, such as a quantum dot solution or quantum dot conjugate solution, is diluted in the composition and stored at 4° C. In certain embodiments, the fluorescence intensity of the diluted fluorescent particle remains substantially the same when stored at 4° C. for at least one month or at least three months. In particular embodiments, a diluted quantum dot conjugate is used to detect a hybridized probe or a protein antigen.

    摘要翻译： 公开了用于稳定纳米颗粒的荧光信号的组合物的实施方案及其使用方法。 在一些实施方案中，组合物具有7至10的pH，并且包括硼酸盐，蛋白质和/或蛋白质水解产物，胺，防腐剂和非离子表面活性剂。 在具体实施方案中，胺是N-乙醇取代的胺，例如乙醇胺，二乙醇胺，三乙醇胺，N-甲基二乙醇胺，N，N-二甲基乙醇胺或其组合。 在一些实施方案中，将荧光颗粒溶液（例如量子点溶液或量子点共轭物溶液）在组合物中稀释并在4℃下储存。在某些实施方案中，稀释的荧光颗粒的荧光强度保持基本相同，当 在4℃下储存至少一个月或至少三个月。 在具体实施方案中，使用稀释的量子点缀合物来检测杂交的探针或蛋白质抗原。

    METHOD FOR CHROMOGENIC DETECTION OF TWO OR MORE TARGET MOLECULES IN A SINGLE SAMPLE
    3.
    发明申请
    METHOD FOR CHROMOGENIC DETECTION OF TWO OR MORE TARGET MOLECULES IN A SINGLE SAMPLE 有权
    用于在单个样品中检测两种或更多种目标分子的方法

    公开(公告)号：US20110136130A1

    公开(公告)日：2011-06-09

    申请号：US13059274

    申请日：2009-08-21

    申请人： Richard Gniewek ,  Michael Farrell ,  Hiroaki Nitta ,  Megan Lehrkamp ,  Jerome Kosmeder ,  Brian Daniel Kelly ,  Fabien Gaire ,  Mary Padilla ,  Thomas Grogan ,  Christopher Bieniarz

    发明人： Richard Gniewek ,  Michael Farrell ,  Hiroaki Nitta ,  Megan Lehrkamp ,  Jerome Kosmeder ,  Brian Daniel Kelly ,  Fabien Gaire ,  Mary Padilla ,  Thomas Grogan ,  Christopher Bieniarz

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6841 ,  G01N33/53 ,  G01N33/581 ,  G01N33/583

    摘要： The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.

    摘要翻译： 本发明提供了用于检测单个组织样品中两种或更多种靶分子的方法和试剂盒，例如用于单一组织样品中的基因和蛋白质双重检测。 方法包括用特异性结合第一靶分子的第一结合部分处理组织样品。 方法还包括在使组织样品与半抗原标记的结合部分接触或同时检测第二靶分子之前或同时使用含有可溶于富电子的芳族化合物的溶液处理组织样品。 在一个实例中，第一靶分子是蛋白质，第二个是核酸序列，第一个靶分子通过免疫组织化学检测，第二个是原位杂交。 所公开的方法由于半抗原标记的特异性结合部分与沉积在第一靶分子附近的不溶性富电子化合物的非特异性结合而减少背景。

    Method for chromogenic detection of two or more target molecules in a single sample
    7.
    发明授权
    Method for chromogenic detection of two or more target molecules in a single sample 有权
    一种样品中两种或更多种靶分子的显色检测方法

    公开(公告)号：US08481270B2

    公开(公告)日：2013-07-09

    申请号：US13059274

    申请日：2009-08-21

    申请人： Richard Gniewek ,  Michael Farrell ,  Hiroaki Nitta ,  Megan Lehrkamp ,  Jerome Kosmeder ,  Brian Daniel Kelly ,  Thomas Grogan ,  Fabien Gaire ,  Mary Padilla ,  Christopher Bieniarz

    发明人： Richard Gniewek ,  Michael Farrell ,  Hiroaki Nitta ,  Megan Lehrkamp ,  Jerome Kosmeder ,  Brian Daniel Kelly ,  Thomas Grogan ,  Fabien Gaire ,  Mary Padilla ,  Christopher Bieniarz

    IPC分类号： C12Q1/68 ,  G01N33/58 ,  G01N33/53

    CPC分类号： C12Q1/6841 ,  G01N33/53 ,  G01N33/581 ,  G01N33/583

    摘要： The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.

    摘要翻译： 本发明提供了用于检测单个组织样品中两种或更多种靶分子的方法和试剂盒，例如用于单一组织样品中的基因和蛋白质双重检测。 方法包括用特异性结合第一靶分子的第一结合部分处理组织样品。 方法还包括在使组织样品与半抗原标记的结合部分接触或同时检测第二靶分子之前或同时使用含有可溶于富电子的芳族化合物的溶液处理组织样品。 在一个实例中，第一靶分子是蛋白质，第二个是核酸序列，第一个靶分子通过免疫组织化学检测，第二个是原位杂交。 所公开的方法由于半抗原标记的特异性结合部分与沉积在第一靶分子附近的不溶性富电子化合物的非特异性结合而减少背景。

    COMPOSITION AND METHOD FOR STABILIZING FLUORESCENT PARTICLES
    8.
    发明申请
    COMPOSITION AND METHOD FOR STABILIZING FLUORESCENT PARTICLES 有权
    用于稳定荧光颗粒的组合物和方法

    公开(公告)号：US20120122094A1

    公开(公告)日：2012-05-17

    申请号：US13382509

    申请日：2011-02-01

    申请人： Eric May ,  Alexandra Nagy ,  Jerome Kosmeder

    发明人： Eric May ,  Alexandra Nagy ,  Jerome Kosmeder

    IPC分类号： G01N21/64 ,  C12Q1/68 ,  B82Y15/00

    CPC分类号： C09K11/025 ,  B82Y15/00 ,  C09K11/883 ,  G01N33/533 ,  G01N33/588 ,  Y10S977/774 ,  Y10S977/902 ,  Y10T436/108331

    摘要： Embodiments of a composition for stabilizing fluorescent signal of nanoparticles and methods for its use are disclosed. In some embodiments, the composition has a pH from 7 to 10 and includes borate, protein and/or protein hydrolysate, an amine, a preservative, and a nonionic surfactant. In particular embodiments, the amine is an N-ethanol substituted amine, such as ethanolamine, diethanolamine, triethanolamine, N-methyldiethanolamine, N,N-dimethylethanolamine, or a combination thereof. In some embodiments, a fluorescent particle solution, such as a quantum dot solution or quantum dot conjugate solution, is diluted in the composition and stored at 4° C. In certain embodiments, the fluorescence intensity of the diluted fluorescent particle remains substantially the same when stored at 4° C. for at least one month or at least three months. In particular embodiments, a diluted quantum dot conjugate is used to detect a hybridized probe or a protein antigen.

    摘要翻译： 公开了用于稳定纳米颗粒的荧光信号的组合物的实施方案及其使用方法。 在一些实施方案中，组合物具有7至10的pH，并且包括硼酸盐，蛋白质和/或蛋白质水解产物，胺，防腐剂和非离子表面活性剂。 在具体实施方案中，胺是N-乙醇取代的胺，例如乙醇胺，二乙醇胺，三乙醇胺，N-甲基二乙醇胺，N，N-二甲基乙醇胺或其组合。 在一些实施方案中，将荧光颗粒溶液（例如量子点溶液或量子点共轭物溶液）在组合物中稀释并在4℃下储存。在某些实施方案中，稀释的荧光颗粒的荧光强度保持基本相同，当 在4℃下储存至少一个月或至少三个月。 在具体实施方案中，使用稀释的量子点缀合物来检测杂交的探针或蛋白质抗原。