公开(公告)号：US11999978B2

公开(公告)日：2024-06-04

申请号：US17279686

申请日：2019-09-25

申请人： Toray Industries, Inc.

发明人： Masateru Ito ,  Yoji Ueda ,  Yuki Takii ,  Mai Yagi

IPC分类号： C12N9/16 ,  C12P19/34

CPC分类号： C12N9/16 ,  C12P19/34 ,  C12Y301/03001

摘要： A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X1/Y)×100≤0.6000 (1); (X2/Y)×100≤0.1800 (2); (X3/Y)×100≤0.2000 (3); (X4/Y)×100≤0.8000 (4); (X5/Y)×100≤1.6000 (5); and (X6/Y)×100≤0.3500 (6), wherein X1 to X6 represent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

公开(公告)号：US11992517B2

公开(公告)日：2024-05-28

申请号：US16368042

申请日：2019-03-28

申请人： NEW YORK SOCIETY FOR THE RUPTURED AND CRIPPLED MAINTAINING THE HOSPITAL FOR SPECIAL SURGERY

发明人： Matthew E. Cunningham ,  Agata Krzyzanowska

IPC分类号： C12N5/077 ,  A61K9/00 ,  A61K31/195 ,  A61K31/675 ,  A61K31/7076 ,  A61K31/7088 ,  A61K31/713 ,  A61K35/32 ,  A61K38/19 ,  A61K38/20 ,  A61K38/46 ,  A61K39/00 ,  A61K45/06 ,  A61K48/00 ,  A61L27/36 ,  A61L27/38 ,  A61L27/54 ,  A61L27/58 ,  A61P19/02 ,  C07K14/47 ,  C12N5/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/55 ,  C12N15/86

CPC分类号： A61K38/465 ,  A61K9/0019 ,  A61K9/0085 ,  A61K31/195 ,  A61K31/675 ,  A61K31/7076 ,  A61K31/7088 ,  A61K31/713 ,  A61K35/32 ,  A61K38/191 ,  A61K38/2006 ,  A61K39/00 ,  A61K45/06 ,  A61K48/00 ,  A61L27/3658 ,  A61L27/3687 ,  A61L27/3821 ,  A61L27/3856 ,  A61L27/3895 ,  A61L27/54 ,  A61L27/58 ,  C07K14/4702 ,  C12N5/0655 ,  C12N15/1138 ,  C12N15/86 ,  A61L2300/412 ,  A61L2300/45 ,  A61L2300/604 ,  A61L2300/64 ,  A61L2400/06 ,  A61L2430/38 ,  C12N2310/122 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/31 ,  C12N2320/32 ,  C12N2501/2301 ,  C12N2501/73 ,  C12N2740/16043 ,  C12Y301/03001 ,  A61K31/195 ,  A61K2300/00 ,  A61K31/675 ,  A61K2300/00 ,  A61K31/7076 ,  A61K2300/00

摘要： This invention relates to compositions and methods for activating and promoting mineralization in tissue that does not normally mineralize, specifically intervertebral discs. The composition comprises agents that increase the expression of the gene that encodes TNAP and/or the activation, amount or activity of TNAP protein, and agents that decrease the expression of ANK and/or ENPP and/or the activation, amount or activity of these proteins. The composition can be in the form of a cell or cells. The invention also relates to methods of using the composition.

公开(公告)号：US20240052327A1

公开(公告)日：2024-02-15

申请号：US18032973

申请日：2021-10-21

申请人： Alexion Pharmaceuticals, Inc.

发明人： Meghan DeWitt ,  Siguang Sui ,  Rahul Godawat ,  Sarah Berendes

IPC分类号： C12N9/16

CPC分类号： C12N9/16 ,  C12Y301/03001 ,  C07K2319/30

摘要： Featured are methods of manufacturing recombinant alkaline phosphatases, such as asfotase alfa, that provide more precise quality control over total sialic acid content (TSAC) concentration in the final product by measuring TSAC concentration during fermentation and adjusting downstream production steps in response.

公开(公告)号：US20230302098A1

公开(公告)日：2023-09-28

申请号：US18018394

申请日：2021-07-29

申请人： The General Hospital Corporation

发明人： Richard A. Hodin

IPC分类号： A61K38/46 ,  A61P1/16

CPC分类号： A61K38/465 ,  A61P1/16 ,  C12Y301/03001

摘要： The present disclosure relates, inter alia, to therapeutic alkaline phosphatases for the treatment of liver fibrosis.

公开(公告)号：US20230190965A1

公开(公告)日：2023-06-22

申请号：US18049296

申请日：2022-10-25

申请人： GENETHON ,  UNIVERSITE D'EVRY VAL D'ESSONNE ,  INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE ,  SORBONNE UNIVERSITÉ

发明人： FEDERICO MINGOZZI ,  GIUSEPPE RONZITTI ,  PATRICE VIDAL

IPC分类号： A61K48/00 ,  A61P3/08 ,  C12N7/00 ,  C12N9/16

CPC分类号： A61K48/0066 ,  A61K48/0033 ,  A61P3/08 ,  C12N7/00 ,  C12N9/16 ,  C12Y301/03001 ,  C12N2750/14121 ,  C12N2750/14132 ,  C12N2750/14143 ,  C12N2750/14145

摘要： The present invention relates to vectors and compositions for the treatment of glycogen storage disease III.

公开(公告)号：US20190209662A1

公开(公告)日：2019-07-11

申请号：US16127159

申请日：2018-09-10

申请人： AM-PHARMA B.V.

发明人： Jacques Salomon Robert Arend ,  Erik Jan Van Den Berg ,  Willem Raaben

IPC分类号： A61K38/46 ,  G01N33/49

CPC分类号： A61K38/465 ,  C12Y301/03001 ,  G01N33/49 ,  G01N2800/52

摘要： The invention relates to the field of medicine and in particular to means and methods for preserving renal function after a treatment with a risk of reducing renal function. The present invention also relates to means and methods for shortening the duration of renal replacement therapy, increasing the creatinine clearance and decreasing the adverse effects of medicaments.

公开(公告)号：US20190185864A1

公开(公告)日：2019-06-20

申请号：US16327396

申请日：2017-08-23

申请人： Akouos, Inc. ,  Georg-August-Universitat Gottingen Stiftung Offentlichen Rechts, Universitatsmedizin, Represen

发明人： Emmanuel John Simons ,  Ellen Reisinger

IPC分类号： C12N15/52 ,  A61P27/16 ,  A61K38/17 ,  C12N15/90 ,  C12N15/86 ,  C12N9/16 ,  C12N5/0793

CPC分类号： C12N15/52 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/103 ,  A01K2267/03 ,  A61K38/1709 ,  A61K48/0075 ,  A61P27/16 ,  C07K14/47 ,  C12N5/062 ,  C12N9/16 ,  C12N15/65 ,  C12N15/86 ,  C12N15/902 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2830/008 ,  C12Y301/03001

摘要： Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject.

公开(公告)号：US20180333470A1

公开(公告)日：2018-11-22

申请号：US15544063

申请日：2016-01-28

申请人： ALEXION PHARMACEUTICALS, INC.

发明人： Tatjana ODRLJIN

IPC分类号： A61K38/46 ,  A61P3/00 ,  A61K31/137 ,  A61K45/06

CPC分类号： A61K38/465 ,  A61K31/137 ,  A61K38/46 ,  A61K45/06 ,  A61P3/00 ,  C12Y301/03001

摘要： Disclosed herein are methods for treating a subject with an alkaline phosphatase deficiency, further comprising monitoring additional analytes, e.g., calcium, parathyroid hormone and/or vitamin D, with treatment modifications as indicated by the levels, e.g., serum levels, of the additional analytes.

公开(公告)号：US20180326017A1

公开(公告)日：2018-11-15

申请号：US15771264

申请日：2015-10-30

申请人： Alexion Pharmaceuticals, Inc.

发明人： Howard M. SAAL ,  Timothy W. VOGEL

IPC分类号： A61K38/46

CPC分类号： A61K38/465 ,  A61B5/4504 ,  A61B2503/04 ,  C12Y301/03001

摘要： The disclosure features methods for treating craniosynostosis in a patient (e.g., a patient having hypophosphatasia (HPP) and exhibiting or likely to have increased intracranial pressure (ICP)) by administering a soluble alkaline phosphatase (sALP) to the patient, e.g., in combination with a cranial surgery, e.g., a cranial vault remodeling procedure.

公开(公告)号：US20180298359A1

公开(公告)日：2018-10-18

申请号：US15970603

申请日：2018-05-03

申请人： IPROGEN BIOTECH INC.

发明人： Keun Ho Lee ,  Leo Yen-Cheng Lin ,  Aikun Wang

IPC分类号： C12N9/16 ,  C07K16/10 ,  C07K14/435 ,  C12N15/85 ,  C07K16/18

CPC分类号： C12N9/16 ,  C07K14/43504 ,  C07K16/1072 ,  C07K16/18 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/10 ,  C07K2319/30 ,  C07K2319/60 ,  C12N15/85 ,  C12Y301/03001

摘要： A secretion signal peptide sequence (SP) in combination with a cleavage inhibition sequence (CIS) fused to a structural gene sequence in a recombinant expression system can be used to express a full length protein with an SP in a cell. Such a fusion protein may be purified to homogeneity from a membrane fraction of the cell. The SP in combination with the CIS is a protein transduction domain that exhibits superior intracellular protein transduction efficiency when the SP precedes the CIS in a N to C-terminus direction.