公开(公告)号：US20100093550A1

公开(公告)日：2010-04-15

申请号：US12445069

申请日：2007-08-24

申请人： John R. Stuelpnagel ,  David L. Barker ,  Jorge Velarde, JR. ,  Steven M. Barnard ,  Michael Graige

发明人： John R. Stuelpnagel ,  David L. Barker ,  Jorge Velarde, JR. ,  Steven M. Barnard ,  Michael Graige

IPC分类号： C40B20/02

CPC分类号： C12N15/1072 ,  C12Q1/6837 ,  C12Q2565/515

摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

摘要翻译： 本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。 该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物 具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品 超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同 复杂混合物中更多的核酸。 还提供了从完整基因组中选择基因组序列的表示样品的方法。 本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于 拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。