公开(公告)号：US06326528B1

公开(公告)日：2001-12-04

申请号：US09417777

申请日：1999-10-14

申请人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard

发明人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard

IPC分类号： A01H100

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8234

摘要： This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, or truncated derivatives of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constructive activity within both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

摘要翻译： 本发明涉及从小麦糊粉基因LtpW1获得的调节区。 该调节区域或该调节区的截短的衍生物可用于在植物的糊粉母细胞内表达感兴趣的异源基因。 此外，本发明涉及在单子叶植物和双子叶植物中表现出建设性活性的截短的LtpW1调节区。 本发明还涉及包含与感兴趣的异源基因可操作地连接的这些调节区的载体，以及包含这些载体的植物细胞培养物和转基因植物。 还公开了使用本发明的调节区制备植物的方法。

公开(公告)号：US06939958B1

公开(公告)日：2005-09-06

申请号：US10110637

申请日：2000-10-13

申请人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard ,  Kamal Malik ,  Teresa Martin-Heller ,  Dan Brown ,  Ming Hu ,  Brian Miki ,  Keqiang Wu

发明人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard ,  Kamal Malik ,  Teresa Martin-Heller ,  Dan Brown ,  Ming Hu ,  Brian Miki ,  Keqiang Wu

IPC分类号： C07K14/415 ,  C12N15/82 ,  C07H21/04

CPC分类号： C12N15/8234 ,  C07K14/415

摘要： This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constitutive activity with both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

摘要翻译： 本发明涉及从小麦糊粉基因LtpW1获得的调节区。 该调节区域，该调节区的截短的衍生物，突变或缺失可用于在植物的糊粉母细胞内表达感兴趣的异源基因。 此外，本发明涉及一种截短的LtpW1调节区，其表现出单子叶植物和双子叶植物的组成活性。 本发明还涉及包含与感兴趣的异源基因可操作地连接的这些调节区的载体，以及包含这些载体的植物细胞培养物和转基因植物。 还公开了使用本发明的调节区制备植物的方法。

公开(公告)号：US6013862A

公开(公告)日：2000-01-11

申请号：US102046

申请日：1998-06-22

申请人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard

发明人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H1/00 ,  C12N5/04 ,  C12N15/09

CPC分类号： C07K14/415 ,  C12N15/8234

摘要： This invention is directed to regulatory elements obtained from a wheat aleurone gene LtpW1. The regulatory element, analogs thereof, or truncated derivatives of this regulatory element, can be used to express heterologous genes of interest within the aleurone cells of a plant. This invention is also directed to vectors comprising these regulatory elements operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory elements of this invention are also disclosed. Furthermore, this invention is directed to a truncated LtpW1 regulatory element that exhibits constitutive activity within plants.

摘要翻译： 本发明涉及从小麦糊粉基因LtpW1获得的调节元件。 其调节元件，其类似物或该调节元件的截短的衍生物可用于在植物的糊粉体细胞内表达感兴趣的异源基因。 本发明还涉及包含与感兴趣的异源基因操作性连接的这些调节元件的载体，以及包含这些载体的植物细胞培养物和转基因植物。 还公开了使用本发明的调节元件制备植物的方法。 此外，本发明涉及在植物中显示组成型活性的截短的LtpW1调节元件。