Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    1.
    发明申请
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号:US20060162009A1

    公开(公告)日:2006-07-20

    申请号:US10743891

    申请日:2003-12-22

    摘要: The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译: 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外,本发明提供了重组表达盒,宿主细胞,转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外,本发明提供了在原核和真核系统中生产APAO酶的方法,用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物,谷物,谷物加工,青贮饲料,粮食作物和动物饲料中真菌毒素的方法。