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    Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops
    6.
    发明授权
    Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops 有权
    改良淀粉生物合成酶基因表达在粮食作物中产生淀粉

    公开(公告)号：US06392120B1

    公开(公告)日：2002-05-21

    申请号：US09345214

    申请日：1999-06-30

    申请人： Karen E. Broglie ,  Jonathan Edward Lightner

    发明人： Karen E. Broglie ,  Jonathan Edward Lightner

    IPC分类号： C12N1582

    CPC分类号： C12N15/1137 ,  C08B30/00 ,  C12N9/1051 ,  C12N15/8245

    摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.

    摘要翻译： 本发明公开了利用cDNA克隆构建用于抑制玉米淀粉合成酶活性的有义和反义基因。 更具体地说，本发明涉及一种控制源自谷类作物谷物的淀粉的淀粉精细结构的方法，包括：（1）制备嵌合基因，其包含编码淀粉合成酶结构基因或其片段的核酸片段，其可操作地连接 在上游侧的任何正义或反义方向，编码引导玉米胚乳组织中的基因表达的启动子的核酸片段，并且在下游侧可操作地连接到编码转录终止的合适调节序列的核酸片段，和（ 2）用所述嵌合基因转化谷类作物，其中所述嵌合基因的表达导致衍生自所述转化的谷类作物的谷物的淀粉的细微结构的改变与源自不具有所述嵌合基因的谷类作物的淀粉的精细结构相比 。

    Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops
    7.
    发明授权
    Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops 有权
    改良淀粉生物合成酶基因表达在粮食作物中产生淀粉

    公开(公告)号：US06570008B1

    公开(公告)日：2003-05-27

    申请号：US09743980

    申请日：2001-01-15

    申请人： Karen E. Broglie ,  Jonathan Edward Lightner

    发明人： Karen E. Broglie ,  Jonathan Edward Lightner

    IPC分类号： C08B3300

    CPC分类号： C12N15/1137 ,  C08B30/00 ,  C12N9/1051 ,  C12N15/8245

    摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endospern tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination; and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.

    摘要翻译： 本发明公开了利用cDNA克隆构建用于抑制玉米淀粉合成酶活性的有义和反义基因。 更具体地说，本发明涉及一种控制源自谷类作物谷物的淀粉的淀粉精细结构的方法，包括：（1）制备嵌合基因，其包含编码淀粉合成酶结构基因或其片段的核酸片段，其可操作地连接 在上游侧的任意一个或反义方向，与编码在玉米胚乳组织中指导基因表达的启动子的核酸片段，并且在下游侧可操作地连接到编码用于转录终止的合适的调控序列的核酸片段; 和（2）用所述嵌合基因转化谷类作物，其中所述嵌合基因的表达导致衍生自所述转化的谷类作物的谷粒的淀粉的细微结构的改变，与来自不具有所述转基因作物的谷类作物的淀粉的精细结构相比 嵌合基因。

    Starch biosynthetic enzymes
    8.
    发明授权
    Starch biosynthetic enzymes 失效
    淀粉生物合成酶

    公开(公告)号：US06255114B1

    公开(公告)日：2001-07-03

    申请号：US09073297

    申请日：1998-05-06

    申请人： Jonathan Edward Lightner ,  John D. Everard

    发明人： Jonathan Edward Lightner ,  John D. Everard

    IPC分类号： C12N1529

    CPC分类号： C12N9/1051 ,  C07K14/415 ,  C12N15/8245 ,  C12N15/8273

    摘要： This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a plant glycogenin or water stress protein. The invention also relates to the construction of chimeric genes encoding all or a portion of a plant glycogenin or water stress protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of a plant glycogenin or water stress protein in a transformed host cell.

    摘要翻译： 本发明涉及编码植物糖原蛋白或水胁迫蛋白的全部或大部分的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码植物糖原蛋白或水应激蛋白的全部或一部分的嵌合基因，其中嵌合基因的表达导致植物糖原蛋白或水胁迫蛋白水平的变化 在转化的宿主细胞中。