公开(公告)号：US20070054289A1

公开(公告)日：2007-03-08

申请号：US11437797

申请日：2006-05-22

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

IPC分类号： C12Q1/00 ,  C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments. Additionally, an improved RNA amplification protocol is provided herein referred to as the CRL amplification protocol which is suitable for reproducibly amplifying all the RNAs expressed by a cell sample, even when only a few cells are available.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。 此外，本文提供了改进的RNA扩增方案，其被称为CRL扩增方案，其适用于可再现地扩增由细胞样品表达的所有RNA，即使只有少数细胞可用。

公开(公告)号：US07858308B2

公开(公告)日：2010-12-28

申请号：US11584580

申请日：2006-10-23

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

IPC分类号： C12Q1/68 ,  C12P19/34 ,  A01N43/04

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20110244464A1

公开(公告)日：2011-10-06

申请号：US12974781

申请日：2010-12-21

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Rosa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20060024693A1

公开(公告)日：2006-02-02

申请号：US11091883

申请日：2005-03-29

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

IPC分类号： C12Q1/68 ,  C12N5/06

CPC分类号： C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20070238111A1

公开(公告)日：2007-10-11

申请号：US11584580

申请日：2006-10-23

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20090180996A1

公开(公告)日：2009-07-16

申请号：US12268095

申请日：2008-11-10

申请人： Zeki Beyhan ,  Arif Kocabas ,  Jose Bernardo Cibelli ,  Steven T. Suhr

发明人： Zeki Beyhan ,  Arif Kocabas ,  Jose Bernardo Cibelli ,  Steven T. Suhr

IPC分类号： A61K35/12 ,  C12N15/87 ,  C12N15/89 ,  C12N15/88 ,  A61P35/00 ,  A61P37/00 ,  A61P31/00 ,  A61P29/00 ,  A61P37/08

CPC分类号： C12N15/1079 ,  C12N2506/00 ,  C12N2799/027

摘要： The invention relates to methods for altering the fate or differentiation status of somatic cells by RNA transfer. These methods can be used to transdifferentiate or dedifferentiate somatic cells of one phenotype or lineage into pluripotent cells or into somatic cells of a different lineage or phenotype.

摘要翻译： 本发明涉及通过RNA转移来改变体细胞的命运或分化状态的方法。 这些方法可用于将一种表型或谱系的体细胞分化或去分化成多能细胞或不同谱系或表型的体细胞。