公开(公告)号：US07858308B2

公开(公告)日：2010-12-28

申请号：US11584580

申请日：2006-10-23

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

IPC分类号： C12Q1/68 ,  C12P19/34 ,  A01N43/04

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20070054289A1

公开(公告)日：2007-03-08

申请号：US11437797

申请日：2006-05-22

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Magalhaes Rosa

IPC分类号： C12Q1/00 ,  C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments. Additionally, an improved RNA amplification protocol is provided herein referred to as the CRL amplification protocol which is suitable for reproducibly amplifying all the RNAs expressed by a cell sample, even when only a few cells are available.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。 此外，本文提供了改进的RNA扩增方案，其被称为CRL扩增方案，其适用于可再现地扩增由细胞样品表达的所有RNA，即使只有少数细胞可用。

公开(公告)号：US20110244464A1

公开(公告)日：2011-10-06

申请号：US12974781

申请日：2010-12-21

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao De Rosa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20070238111A1

公开(公告)日：2007-10-11

申请号：US11584580

申请日：2006-10-23

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US20060024693A1

公开(公告)日：2006-02-02

申请号：US11091883

申请日：2005-03-29

申请人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

发明人： Jose Cibelli ,  Javier Crosby ,  Emilio Fernandez ,  Arif Kocabas ,  Guilherme Jordao Rosa

IPC分类号： C12Q1/68 ,  C12N5/06

CPC分类号： C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158

摘要： A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments.

摘要翻译： 提供确定女性受试者是否产生“怀孕受精”卵母细胞的遗传手段。 该方法包括检测在源于妊娠合格卵母细胞的卵丘细胞中以特征水平（上调或下调）表达的一种或多种基因的表达水平。 这种特征性基因表达水平或本文称为“怀孕签名”的模式也可以用于鉴定有潜在病症的受试者，其损害或预防可行妊娠的发展，例如绝经前期，其他激素功能障碍， 卵巢功能障碍，卵巢囊肿，癌症或其他细胞增殖障碍，自身免疫性疾病等。 包含“怀孕特征”基因或相应多肽的微阵列提供本发明的另一优选方面。 此外，本发明可以用于衍生动物模型，例如非人灵长类动物模型，以评估推定的女性生育力治疗的功效。

公开(公告)号：US07600972B2

公开(公告)日：2009-10-13

申请号：US10699060

申请日：2003-10-31

申请人： Edward Durell Benjamin ,  Jeffrey John Butkiewicz ,  Mark Steven Honkomp ,  Stephen Paul Wassynger ,  Emilio Fernandez ,  Carlos Alberto Collado

发明人： Edward Durell Benjamin ,  Jeffrey John Butkiewicz ,  Mark Steven Honkomp ,  Stephen Paul Wassynger ,  Emilio Fernandez ,  Carlos Alberto Collado

IPC分类号： F01D5/18

CPC分类号： F01D5/187 ,  F05D2240/81 ,  F05D2260/201 ,  Y10T29/49321

摘要： A method and apparatus for a rotor assembly for gas turbine engine are provided. A first rotor blade including an airfoil, a platform, a shank, an internal cavity, and a dovetail is provided, wherein the airfoil extends radially outward from the platform, which includes a radially outer surface and a radially inner surface, the shank extends radially inward from the platform, and the dovetail extends from the shank, such that the internal cavity is defined by the airfoil, the platform, the shank, and the dovetail. The first rotor blade is coupled to a rotor shaft such that during engine operation, cooling air is channeled from the cavity through an impingement cooling circuit for impingement cooling the first rotor blade platform radially inner surface, and a second rotor blade is coupled to the rotor shaft such that a platform gap is defined between the first and second rotor blade platforms.

摘要翻译： 提供了一种用于燃气轮机的转子组件的方法和装置。 提供了包括翼型件，平台，柄，内腔和燕尾的第一转子叶片，其中所述翼型件从所述平台径向向外延伸，所述平台包括径向外表面和径向内表面，所述柄径向延伸 从该平台向内，并且该燕尾榫从该柄部延伸，使得该内腔由翼型件，平台，柄部和燕尾形形成。 第一转子叶片联接到转子轴，使得在发动机操作期间，冷却空气通过冲击冷却回路从空腔引导，用于冲击冷却第一转子叶片平台径向内表面，并且第二转子叶片联接到转子 轴，使得在第一和第二转子叶片平台之间限定平台间隙。

公开(公告)号：US20080145207A1

公开(公告)日：2008-06-19

申请号：US11638818

申请日：2006-12-14

申请人： Patrick Mohr ,  Daniel Nowak ,  Emilio Fernandez ,  Michael Arnett ,  David Williams ,  Carlos Collado

发明人： Patrick Mohr ,  Daniel Nowak ,  Emilio Fernandez ,  Michael Arnett ,  David Williams ,  Carlos Collado

IPC分类号： F01D11/12 ,  F04D29/08

CPC分类号： F01D5/225 ,  F05C2201/0463 ,  F05D2300/506

摘要： A system in a turbine engine for preventing wear on a tip shroud of a turbine blade that includes a pocket formed in a contact surface of the tip shroud and a plug that fits within the pocket and has a durable outer surface. The durable outer surface may include a cobalt-based hardfacing powder. The pocket may be machined out of the contact surface, and the plug may include a plug of predetermined size that fits snugly into the pocket. In some embodiments, the durable outer surface may substantially align with the contact surface after the plug is fitted into the pocket. In other embodiments, the durable outer surface may remain slightly raised from the contact surface after the plug has been fitted into the pocket.

摘要翻译： 一种涡轮发动机中的系统，用于防止涡轮机叶片的尖端护罩上的磨损，该系统包括形成在尖端护罩的接触表面中的凹穴和装配在口袋内并且具有耐用的外表面的塞子。 耐用的外表面可以包括钴基硬化粉末。 口袋可以从接触表面加工出来，并且塞子可以包括预先规定尺寸的塞子，该栓塞紧贴地配合到口袋中。 在一些实施例中，在将塞子装配到口袋中之后，耐用的外表面可以与接触表面大致对准。 在其他实施例中，在插头已经装配到口袋中之后，耐用的外表面可以保持从接触表面稍微升高。

公开(公告)号：US20050095128A1

公开(公告)日：2005-05-05

申请号：US10699060

申请日：2003-10-31

申请人： Edward Benjamin ,  Jeffrey Butkiewicz ,  Mark Honkomp ,  Stephen Wassynger ,  Emilio Fernandez ,  Carlos Collado

发明人： Edward Benjamin ,  Jeffrey Butkiewicz ,  Mark Honkomp ,  Stephen Wassynger ,  Emilio Fernandez ,  Carlos Collado

IPC分类号： F01D5/18 ,  F01D5/30 ,  F02C7/18 ,  B63H1/14

CPC分类号： F01D5/187 ,  F05D2240/81 ,  F05D2260/201 ,  Y10T29/49321

摘要： A method facilitates assembling a rotor assembly for gas turbine engine. The method includes providing a first rotor blade including an airfoil, a platform, a shank, an internal cavity, and a dovetail, wherein the airfoil extends radially outward from the platform, which includes a radially outer surface and a radially inner surface, the shank extends radially inward from the platform, and the dovetail extends from the shank, such that the internal cavity is defined by the airfoil, the platform, the shank, and the dovetail. The method also includes coupling the first rotor blade to a rotor shaft such that during engine operation, cooling air is channeled from the cavity through an impingement cooling circuit for impingement cooling the first rotor blade platform radially inner surface, and coupling a second rotor blade to the rotor shaft such that a platform gap is defined between the first and second rotor blade platforms.

摘要翻译： 一种方法有利于组装用于燃气涡轮发动机的转子组件。 该方法包括提供包括翼型件，平台，柄，内部空腔和燕尾榫的第一转子叶片，其中所述翼型件从所述平台径向向外延伸，所述平台包括径向外表面和径向内表面，所述柄 从平台径向向内延伸，并且燕尾榫从柄部延伸，使得内部空腔由翼型件，平台，柄部和燕尾形构成。 该方法还包括将第一转子叶片耦合到转子轴，使得在发动机运转期间，冷却空气通过冲击冷却回路从空腔引导，用于冲击冷却第一转子叶片平台径向内表面，并将第二转子叶片耦合到 转子轴使得在第一和第二转子叶片平台之间限定平台间隙。

公开(公告)号：US4618391A

公开(公告)日：1986-10-21

申请号：US636763

申请日：1984-08-01

申请人： Mario Torti ,  Emilio Fernandez

发明人： Mario Torti ,  Emilio Fernandez

IPC分类号： B23D36/00 ,  B31B50/20 ,  B26D5/20

CPC分类号： B23D36/0058 ,  B31B1/00 ,  B31B2201/143 ,  Y10T156/12 ,  Y10T83/148 ,  Y10T83/152 ,  Y10T83/159 ,  Y10T83/4691

摘要： Device for bringing into register a tool mounted on a rotary cylinder for processing products in sheet form, such as a tool for cutting out, creasing, or printing sheets of cardboard in a machine for making and printing corrugated cardboard boxes, comprising a coder (22) providing information representing the theoretical advance of the sheets (10, 11), a coder (23) providing information representing the angle of adjustment given to the tool holder (1), a detector (20) of the passage of a reference mark (15) on the sheet (11) to a point situated upstream of the tool holder, a detector (36) of the theoretical passage of this reference mark at a selected distance slightly downstream of the first detector (20), and a computer (33) supplying correction commands to the correction motor (8) of the tool holder in order that the tool will arrive in phase with the incident sheet (11).

摘要翻译： 用于将安装在旋转圆筒上的工具安装在用于处理片状产品的工具的装置，例如用于在用于制造和印刷瓦楞纸箱的机器中切割，压痕或打印纸板的工具，包括编码器（22） ）提供表示纸张（10,11）的理论进展的信息，提供表示向工具架（1）提供的调整角度的信息的编码器（23），参考标记通过的检测器（20） 15）位于工具架上游的位置处，该参考标记的理论通道的检测器（36）在第一检测器（20）的稍微下游的选定距离处，以及计算机（33） ）向工具架的校正马达（8）提供校正命令，以便工具将与入射片（11）同相到达。

公开(公告)号：US20050196277A1

公开(公告)日：2005-09-08

申请号：US10708417

申请日：2004-03-02

申请人： John Wang ,  Mark Bailey ,  Robert Berry ,  Emilio Fernandez

发明人： John Wang ,  Mark Bailey ,  Robert Berry ,  Emilio Fernandez

IPC分类号： F01D5/28 ,  F01D5/14 ,  F01D5/18 ,  F01D5/20 ,  F01D25/00 ,  F02C7/00 ,  F03B3/12

CPC分类号： F01D5/20

摘要： A tip cap for a turbine blade. The tip cap may include a HS-188 sheet material with a thickness of less than about 0.079 inches (about 2 millimeters) and a number of holes positioned in the sheet material.

摘要翻译： 涡轮叶片的顶盖。 尖端帽可以包括厚度小于约0.079英寸（约2毫米）的HS-188片材和位于片材中的多个孔。