公开(公告)号：US5215922A

公开(公告)日：1993-06-01

申请号：US831190

申请日：1992-02-05

申请人： Joseph D. Artiss ,  Bradley R. Morgan ,  Bennie Zak

发明人： Joseph D. Artiss ,  Bradley R. Morgan ,  Bennie Zak

IPC分类号： C12Q1/44 ,  G01N31/22 ,  G01N33/84

CPC分类号： G01N31/22 ,  C12Q1/44 ,  G01N33/84 ,  G01N2400/18 ,  Y10T436/106664 ,  Y10T436/107497

摘要： A colorimetric, particularly spectrophotometric, method and compositions for determining calcium levels in blood serum in the presence of magnesium and other serum ions is described. The method uses an aqueous composition with a pH between about 7.5 and 10 with arsenazo III to complex calcium and a hydroxyquinoline selected from the group consisting of 8-hydroxyquinoline and 8-hydroxyquinoline-5-sulfonate to complex magnesium. Lipase and a cyclodextrin can be used as reagents to clear triglycerides in the serum which can provide turbidity and false results in some instances. The method is particularly useful for human serum.

摘要翻译： 描述了在镁和其它血清离子存在下测定血清中钙水平的比色，特别是分光光度法和组合物。 该方法使用具有约7.5至10的pH的含水组合物与砷砷化合物III配合，并将选自8-羟基喹啉和8-羟基喹啉-5-磺酸盐的羟基喹啉复合成镁。 脂肪酶和环糊精可用作清除血清中的甘油三酯的试剂，可在一些情况下提供浊度和假结果。 该方法对人血清特别有用。