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    Non-aggregated fluorescent conjugates and the process for their preparation
    1.
    发明授权
    Non-aggregated fluorescent conjugates and the process for their preparation 失效
    非聚集荧光共轭物及其制备方法

    公开(公告)号：US6120987A

    公开(公告)日：2000-09-19

    申请号：US95471

    申请日：1998-06-10

    申请人： Daniel Aspe

    发明人： Daniel Aspe

    IPC分类号： C07H21/00 ,  G01N33/533 ,  C12Q1/00 ,  C07H21/02

    CPC分类号： C07H21/00 ,  G01N33/533 ,  G01N2400/18 ,  Y10S436/80

    摘要： The invention relates to a process for the preparation of a fluorescent conjugate between a carrier molecule possessing at least one amino, hydroxyl, carboxyl and/or sulfhydryl group and a fluorophoric reagent possessing at least one functional group capable of reacting with said amino, hydroxyl, carboxyl and/or sulfhydryl group(s), which consists in bringing said carrier molecule and said fluorophoric reagent into contact with an aqueous solution of a water-soluble macrocycle.The invention further relates to the conjugates obtained by this process and to their use.

    摘要翻译： 本发明涉及一种在具有至少一个氨基，羟基，羧基和/或巯基的载体分子和具有至少一个能够与所述氨基，羟基和/或巯基反应的官能团的荧光试剂之间制备荧光偶联物的方法， 羧基和/或巯基，其包括使所述载体分子和所述荧光试剂与水溶性大环的水溶液接触。 本发明还涉及通过该方法获得的缀合物及其用途。

    Assay method for amylase activity and method of producing maltose dehydrogenase for use therein
    3.
    发明授权
    Assay method for amylase activity and method of producing maltose dehydrogenase for use therein 失效
    淀粉酶活性的测定方法和生产用于其中的麦芽糖脱氢酶的方法

    公开(公告)号：US4427771A

    公开(公告)日：1984-01-24

    申请号：US311263

    申请日：1981-10-14

    申请人： Hideo Misaki ,  Eiji Muramatsu ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    发明人： Hideo Misaki ,  Eiji Muramatsu ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    IPC分类号： C12N9/04 ,  C12Q1/40 ,  C12N1/20 ,  C12Q1/32 ,  C12R1/11

    CPC分类号： C12N9/0006 ,  C12Q1/40 ,  G01N2400/18 ,  Y10S435/837

    摘要： An assay method for amylase activity in a biological specimen such as serum, saliva or urine. The enzyme amylase in the specimen is used to decompose a substrate which is a glucose polymer having a modified reducing terminal glucose residue or a cyclic glucose polymer. A component of the decomposed substrate is measured as an indication of amylase activity in the specimen. The residue may be amylose, amylopectin, starch, starch hydrolyzate, an etherified reducing terminal, an esterified reducing terminal, gluconolactone or a gluconic acid residue or its derivative. Decomposed substrate assay may be effected by contacting the same with maltose dehydrogenase and NAD or NADP, whereupon the assay is performed by measuring the amount of reduced NAD or reduced NADP, by reacting the same with reduced-form hydrogen transport colorimetric reaction reagent. This reagent may be a tetrazolium salt and diaphorase, or tetrazolium salt and phenazinemethosulfate. To remove pre-existing glucose and maltose present in the specimen, the specimen may be pretreated with alpha-glucosidase or kinase in the presence of Mg.sup.++ and ATP, the kinase being for example hexokinase. The preferred maltose dehydrogenase is produced by culturing Bacillus megaterium B-0779 FERM-P No. 5662.

    摘要翻译： 生物样品如血清，唾液或尿液中淀粉酶活性的测定方法。 试样中的酶淀粉酶用于分解具有修饰的还原性末端葡萄糖残基或环状葡萄糖聚合物的葡萄糖聚合物的底物。 测量分解的底物的组分作为样品中淀粉酶活性的指示。 残基可以是直链淀粉，支链淀粉，淀粉，淀粉水解物，醚化还原末端，酯化还原末端，葡萄糖酸内酯或葡萄糖酸残基或其衍生物。 分解的底物测定可以通过使其与麦芽糖脱氢酶和NAD或NADP接触来实现，因此通过使其与还原形式的氢转运比色反应试剂反应来测量还原的NAD或还原的NADP的量来进行测定。 该试剂可以是四唑盐和心律黄素，或四唑鎓盐和吩嗪甲硫酸盐。 为了除去样品中存在的现有葡萄糖和麦芽糖，可以在Mg ++和ATP存在下用α-葡糖苷酶或激酶预处理样品，所述激酶例如己糖激酶。 优选的麦芽糖脱氢酶是通过培养巨大芽孢杆菌B-0779 FERM-P No.5662产生的。

    Sensor comprising a hydrophilic matrix material
    5.
    发明申请
    Sensor comprising a hydrophilic matrix material 审中-公开
    包含亲水基质材料的传感器

    公开(公告)号：US20020131900A1

    公开(公告)日：2002-09-19

    申请号：US10043062

    申请日：2002-01-08

    发明人： Niels-Henrik Jensen

    IPC分类号： G01N021/00

    CPC分类号： G01N33/84 ,  C12Q1/002 ,  G01N2021/7786 ,  G01N2400/18

    摘要： A sensor for measuring an analyte in a biological sample, the sensor including a hydrophilic and/or water-swellable polymeric matrix material at least one portion of which includes an analyte-sensitive indicator compound and a cyclic compound which has a three-dimensional structure forming a hydrophobic inner cavity and a hydrophilic exterior surface.

    摘要翻译： 一种用于测量生物样品中的分析物的传感器，所述传感器包括亲水和/或水可溶胀聚合物基质材料，其至少一部分包括分析物敏感指示剂化合物和形成三维结构的环状化合物 疏水性内腔和亲水性外表面。

    Method for analyzing intracellular components
    6.
    发明授权
    Method for analyzing intracellular components 有权
    分析细胞内成分的方法

    公开(公告)号：US06238857B1

    公开(公告)日：2001-05-29

    申请号：US09555682

    申请日：2000-06-02

    申请人： Noriaki Hattori ,  Keiko Yajitate ,  Motoo Nakajima ,  Seiji Murakami

    发明人： Noriaki Hattori ,  Keiko Yajitate ,  Motoo Nakajima ,  Seiji Murakami

    IPC分类号： C12Q100

    CPC分类号： G01N33/66 ,  C12Q1/66 ,  C12Q2304/60 ,  G01N2400/16 ,  G01N2400/18

    摘要： A method for analyzing an intracellular component comprising the following steps, and a reagent kit comprising (a) an extraction reagent, (b) branched dextrin or a derivative thereof, and (c) a reagent for analyzing an intracellular component: (1) step of adding an extraction reagent to a sample containing cells to extract the intracellular component; (2) step of adding branched dextrin or a derivative thereof to the sample containing the extraction reagent; and (3) step of analyzing the extracted intracellular component.

    摘要翻译： 一种分析细胞内成分的方法，包括以下步骤，以及试剂盒，其包含（a）提取试剂，（b）支链糊精或其衍生物，和（c）用于分析细胞内成分的试剂：（1） 向含有细胞的样品中添加提取试剂以提取细胞内成分;（2）将含有支链糊精或其衍生物的支链糊精或其衍生物加入到含有提取试剂的样品中的步骤; 和（3）分析提取的细胞内成分的步骤。

    Assay reagents
    7.
    发明授权
    Assay reagents 失效
    测定试剂

    公开(公告)号：US5736353A

    公开(公告)日：1998-04-07

    申请号：US261007

    申请日：1994-06-14

    申请人： Elizabeth Anne Weavers ,  Michael Joseph Powell

    发明人： Elizabeth Anne Weavers ,  Michael Joseph Powell

    IPC分类号： C12N9/08 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/535

    CPC分类号： C12N9/0065 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/535 ,  C12Q2326/12 ,  G01N2400/18 ,  Y10S435/975

    摘要： The present invention relates to a peroxidase-containing reagent comprising a buffered aqueous solution of a peroxidase conjugate and a substrate for the peroxidase, in the absence of peroxide. The invention further relates to a method of stabilizing the peroxidase activity of a buffered aqueous solution containing a peroxidase conjugate. Further, the invention relates to a tetramethylbenzidine peroxidase substrate reagent and to a method of preparing same.

    摘要翻译： 本发明涉及含有过氧化物酶的试剂，其在不存在过氧化物的情况下，包含过氧化物酶缀合物的缓冲水溶液和过氧化物酶底物。 本发明还涉及稳定含有过氧化物酶缀合物的缓冲水溶液的过氧化物酶活性的方法。 此外，本发明涉及四甲基联苯胺过氧化物酶底物试剂及其制备方法。

    Protein detection using modified cyclodextrins
    8.
    发明授权
    Protein detection using modified cyclodextrins 有权
    使用改性环糊精的蛋白质检测

    公开(公告)号：US09285359B2

    公开(公告)日：2016-03-15

    申请号：US14455609

    申请日：2014-08-08

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Clayton T. McKee ,  William Strong ,  Lee Olech ,  Thomas Berkelman ,  Christopher Belisle

    IPC分类号： G01N33/53 ,  G01N33/58 ,  G01N33/68 ,  G01N27/447

    CPC分类号： G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

    摘要： A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.

    摘要翻译： 提供了一种使用共价连接至少一个标签的环糊精来检测蛋白质的方法。 环糊精可以通过在其疏水腔中螯合蛋白质的芳香族氨基酸侧链而与蛋白质结合。 将蛋白质与环糊精接触后，可以直接检测标记物，或者可以与试剂进行化学相互作用以形成可检测的产物。 标记可以包括吲哚部分，其可以在暴露于紫外光时与卤素取代的有机化合物反应，从而变成荧光。 或者，标记可以包括生物素部分，其可以结合结合配偶体，例如抗生物素蛋白或其变体，以形成可检测的分子复合物。 标记的环糊精可用于本方法中以检测电泳凝胶或印迹膜上的目的蛋白质。 蛋白质，特别是色氨酸的芳香族氨基酸残基由于环糊精的螯合而保护免受化学修饰，使得这些方法与需要完整蛋白质的下游应用相容。 本文还提供了用于检测蛋白质的组合物，试剂盒和电泳凝胶。

    Relating to assay reagents
    9.
    发明授权
    Relating to assay reagents 失效
    与测定试剂有关

    公开(公告)号：US5874232A

    公开(公告)日：1999-02-23

    申请号：US979792

    申请日：1997-11-26

    申请人： Elizabeth Anne Weavers ,  Michael Joseph Powell

    发明人： Elizabeth Anne Weavers ,  Michael Joseph Powell

    IPC分类号： C12N9/08 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/535 ,  C12Q1/00 ,  G01N33/53

    CPC分类号： C12N9/0065 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/535 ,  C12Q2326/12 ,  G01N2400/18 ,  Y10S435/975

    摘要： We have discovered that the stability of a dilute peroxidase-containing solution is greatly enhanced by the addition of a specific substrate for the peroxidase, in the absence of peroxide. We provide a peroxidase-containing reagent consisting of a buffered aqueous solution comprising a peroxidase or a peroxidase conjugate and a specific substrate for the peroxidase, in the absence of peroxide. The peroxidase may be a free peroxidase but for assay purposes is preferably a peroxidase bound to a specific binding component of an assay, to form a peroxidase conjugate. The peroxidase conjugate is preferably a conjugate between a peroxidase and an antigen or an antibody, most preferably an antibody. The peroxidase is preferably horseradish peroxidase.

    摘要翻译： 我们已经发现，在不存在过氧化物的情况下，通过添加过氧化物酶的特定底物，可以大大提高稀释过氧化物酶溶液的稳定性。 在不存在过氧化物的情况下，我们提供由含有过氧化物酶或过氧化物酶缀合物的缓冲水溶液和过氧化物酶特异性底物组成的含过氧化物酶的试剂。 过氧化物酶可以是游离过氧化物酶，但是为了测定目的优选是结合于测定的特异性结合组分的过氧化物酶，以形成过氧化物酶缀合物。 过氧化物酶缀合物优选是过氧化物酶与抗原或抗体之间的缀合物，最优选抗体。 过氧化物酶优选是辣根过氧化物酶。