Nodulation gene promoter
    3.
    发明授权
    Nodulation gene promoter 失效
    结瘤基因启动子

    公开(公告)号:US5484718A

    公开(公告)日:1996-01-16

    申请号:US875300

    申请日:1986-06-17

    摘要: The invention includes a consensus nucleotide sequence found in the promoter regions of those Rhizobium and Bradyrhizobium nodulation genes which are activated by chemical inducer(s) in legume exudate. A promoter comprising the consensus sequence is a legume exudate-inducible promoter. A recombinant gene comprising a structural gene and a legume exudate-inducible promoter is selectively expressed in the presence of legume exudate or chemical inducer(s) and a functional nodD gene. The consensus nucleotide sequence isATCCAYNNYGYRGATGNWYKYKATCSAAWCAATCRATTTTACCARWYYKNSRR whereN is A, G, C or TY is C or TR is A or GW is A or TK is G or T andS is C or G.

    摘要翻译: 本发明包括在由豆科渗出物中的化学诱导剂活化的那些根瘤菌和Bradyrhizobium结瘤基因的启动子区域中发现的共有核苷酸序列。 包含共有序列的启动子是豆科渗出物诱导型启动子。 在豆类渗出物或化学诱导剂和功能性nodD基因的存在下,选择性地表达包含结构基因和豆科渗出物诱导型启动子的重组基因。 共有核苷酸序列为ATCCAYNNYGYRGATGNWYKYKATCSAAWCAATCRATTTTACCARWYYKNSRR,其中N为A,G,C或T Y为C或T R为A或G W为A或T K为G或T,S为C或G。