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1.发明申请Methods of detecting cancer cells and use of same for diagnosing and monitoring treatment of the disease 失效检测癌细胞的方法及其用途，用于诊断和监测疾病的治疗公开(公告)号：US20090232740A1
公开(公告)日：2009-09-17
申请号：US12379974
申请日：2009-03-05
IPC分类号： A61K49/00 , G01N33/574 , A61P35/00
CPC分类号： G01N33/5011 , C12Q1/42
摘要： A method of detecting cancer cells is disclosed. The method comprises (a) contacting the cell with a substrate for an enzyme under conditions wherein the enzyme catalyzes a reaction of the cell with said substrate, so as to generate a product capable of producing an electrical signal; and (b) measuring a level of the electrical signal, wherein a difference in a level of said electrical signal compared to a predetermined threshold is indicative of a cancer cell. The method may be adapted for diagnosis, monitoring a cancer therapy, identifying agents capable of treating cancer and individually optimizing a cancer therapy.
摘要翻译： 公开了一种检测癌细胞的方法。 该方法包括（a）在酶催化细胞与所述底物的反应的条件下，使细胞与酶底物接触，从而产生能产生电信号的产物; 和（b）测量电信号的电平，其中所述电信号的电平差与预定阈值相比指示癌细胞。 该方法可适用于诊断，监测癌症治疗，鉴定能够治疗癌症并单独优化癌症治疗的药剂。
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2.发明授权公开(公告)号：US08530179B2
公开(公告)日：2013-09-10
申请号：US13551642
申请日：2012-07-18
IPC分类号： C12Q1/42
CPC分类号： G01N33/5011 , C12Q1/42
摘要： A method of detecting cancer cells is disclosed. The method comprises (a) contacting the cell which is suspicious of a cancerous or undifferentiated phenotype with 4-aminophenyl phosphate (p-APP) under conditions wherein alkaline phosphatase of the cell catalyzes a reaction of the cell with the p-APP, so as to generate a product capable of producing an electrical signal; and (b) measuring a level of the electrical signal, wherein a difference in a level of the electrical signal compared to a predetermined threshold is indicative of a cancer cell.
摘要翻译： 公开了一种检测癌细胞的方法。 该方法包括（a）在细胞的碱性磷酸酶催化细胞与p-APP的反应的条件下，将可疑癌症或未分化表型的细胞与4-氨基苯基磷酸（p-APP）接触，以便 以产生能够产生电信号的产品; 和（b）测量电信号的电平，其中与预定阈值相比较的电信号电平的差异指示癌细胞。
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3.发明授权公开(公告)号：US08268577B2
公开(公告)日：2012-09-18
申请号：US12379974
申请日：2009-03-05
IPC分类号： C12Q1/42
CPC分类号： G01N33/5011 , C12Q1/42
摘要： A method of detecting cancer cells is disclosed. The method comprises (a) contacting the cell with a substrate for an enzyme under conditions wherein the enzyme catalyzes a reaction of the cell with the substrate, so as to generate a product capable of producing an electrical signal; and (b) measuring a level of the electrical signal, wherein a difference in a level of the electrical signal compared to a predetermined threshold is indicative of a cancer cell. The method may be adapted for diagnosis, monitoring a cancer therapy, identifying agents capable of treating cancer and individually optimizing a cancer therapy.
摘要翻译： 公开了一种检测癌细胞的方法。 该方法包括：（a）在酶催化细胞与底物的反应的条件下使细胞与酶底物接触，从而产生能产生电信号的产物; 和（b）测量电信号的电平，其中与预定阈值相比较的电信号电平的差异指示癌细胞。 该方法可适用于诊断，监测癌症治疗，鉴定能够治疗癌症并单独优化癌症治疗的药剂。
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公开(公告)号：US20090061524A1
公开(公告)日：2009-03-05
申请号：US11918477
申请日：2006-04-11
申请人： Judith Rishpon , Tova Neufeld
发明人： Judith Rishpon , Tova Neufeld
CPC分类号： G01N33/5438 , C12Q1/003
摘要： Low-cost, non-toxic and fast immunoassay systems (immunosensors) and uses thereof as analytical and diagnostic tools for detecting an immune response in a subject are disclosed. The systems and methods disclosed are based on recording an electrochemical signal which is generated proportionally to an enzymatic cascade reaction (enzyme-channeling) upon detecting an analyte, and therefore can be used to determine the titer level of an antibody analyte in a liquid sample such as artificial media, serum or blood both qualitatively and quantitatively, in a one-step and separation free immunoassay. Systems and methods based on recording an electrochemical signal which is generated proportionally to an enzymatic cascade reaction (enzyme-channeling) upon detecting an analyte, which utilize a non-toxic secondary substrate such as acetaminophen are also disclosed.
摘要翻译： 公开了低成本，无毒和快速免疫测定系统（免疫传感器）及其用作检测受试者中的免疫应答的分析和诊断工具。 所公开的系统和方法基于记录在检测到分析物时与酶级联反应（酶 - 引导）成比例地产生的电化学信号，因此可用于确定液体样品中抗体分析物的滴度水平，例如 作为人造介质，血清或血液在定性和定量上，在一步和无分离免疫测定中。 还公开了基于在检测利用无毒二级底物如对乙酰氨基酚的分析物时与酶级联反应（酶 - 引导）成比例地产生的电化学信号的系统和方法。
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公开(公告)号：US07879212B2
公开(公告)日：2011-02-01
申请号：US11591613
申请日：2006-11-02
申请人： Miri Yemini , Meital Reches , Judith Rishpon , Ehud Gazit
发明人： Miri Yemini , Meital Reches , Judith Rishpon , Ehud Gazit
IPC分类号： G01N27/327
CPC分类号： C25B11/0447
摘要： An electrode coated with peptide nanostructures, composed of self-assembled peptides, is disclosed. The electrode is capable of conducting a response current resulting from an electrochemical reaction. The electrode can form a part of an electrochemical cell, a detector and a sensor array. Methods utilizing an electrochemical cell, a detector or a sensor array comprising the electrode for detecting an Analyte in a sample and kits containing same are also disclosed.
摘要翻译： 公开了由自组装肽组成的涂有肽纳米结构的电极。 电极能够传导由电化学反应产生的响应电流。 电极可以形成电化学电池的一部分，检测器和传感器阵列。 还公开了利用电化学电池，检测器或传感器阵列的方法，其包括用于检测样品中的分析物的电极和包含其的试剂盒。
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公开(公告)号：US06391549B1
公开(公告)日：2002-05-21
申请号：US09283916
申请日：1999-04-01
申请人： Eliora Z. Ron , Judith Rishpon , Israel Biran , Reuven Babai
发明人： Eliora Z. Ron , Judith Rishpon , Israel Biran , Reuven Babai
IPC分类号： G01N3353
摘要： A method and system for detecting a parameter of a host cell, a cell culture or medium is provided. A host cell is transfected with an expressible DNA sequence which is under expression control of an inducible promoter sequence. The promoter sequence is inducible in correlation with the parameter, the expressible sequence encoding an enzymatically active product which can catalyze a reaction giving rise to an electrical signal which is detectable in an electrochemical measurement. An electrical signal determined in an electrochemical system then serves as an indication for the parameter.
摘要翻译： 提供了用于检测宿主细胞，细胞培养物或培养基的参数的方法和系统。 用可诱导的启动子序列的表达控制下的可表达的DNA序列转染宿主细胞。 启动子序列可以与参数相关，可编码酶活性产物的可表达序列可以催化产生可在电化学测量中检测到的电信号的反应。 然后在电化学系统中确定的电信号用作参数的指示。
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公开(公告)号：US5227042A
公开(公告)日：1993-07-13
申请号：US883746
申请日：1992-05-15
IPC分类号： C12Q1/00
CPC分类号： C12Q1/003 , C12Q1/005 , Y10S435/817
摘要： An enzyme electrode is prepared with a composite coating on an electrical conductor. The composite coating is formed from a casting solution of a perfluorosulfonic acid polymer, an enzyme, and a carbon supported catalyst. The solution may be cast directly on the conductor surface or may be formed as a membrane and applied to the surface. The perfluorosulfonic acid ionomer formed from the casting solution provides an insoluble biocompatible protective matrix for the enzyme and acts to retain the enzyme for long term availability in the electrode structure. The carbon supported catalyst provides catalytic sites throughout the layer for the oxidation of hydrogen peroxide from the enzyme reactions. The carbon support then provides a conductive path for establishing an electrical signal to the electrical conductor. In one embodiment, the electrical conductor is a carbon cloth that permits oxygen or other gas to be introduced to the perfluorosulfonic polymer to promote the enzyme reaction independent of oxygen in the solution being tested.
摘要翻译： 用电导体上的复合涂层制备酶电极。 复合涂层由全氟磺酸聚合物，酶和负载碳的催化剂的流延溶液形成。 溶液可以直接浇铸在导体表面上，或者可以形成为膜并施加到表面上。 由浇铸溶液形成的全氟磺酸离聚物为酶提供了不溶的生物相容性保护基质，并且用于保留酶在电极结构中的长期可利用性。 碳载体催化剂在整个层中提供催化位点，用于从酶反应中氧化过氧化氢。 碳载体然后提供用于向电导体建立电信号的导电路径。 在一个实施方案中，电导体是碳布，其允许将氧气或其它气体引入全氟磺酸聚合物以促进酶反应，而与所测溶液中的氧无关。
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公开(公告)号：US20070138007A1
公开(公告)日：2007-06-21
申请号：US11591613
申请日：2006-11-02
申请人： Miri Yemini , Meital Reches , Judith Rishpon , Ehud Gazit
发明人： Miri Yemini , Meital Reches , Judith Rishpon , Ehud Gazit
IPC分类号： C25B11/04
CPC分类号： C25B11/0447
摘要： An electrode coated with peptide nanostructures, composed of self-assembled peptides, is disclosed. The electrode is capable of conducting a response current resulting from an electrochemical reaction. The electrode can form a part of an electrochemical cell, a detector and a sensor array. Methods utilizing an electrochemical cell, a detector or a sensor array comprising the electrode for detecting an Analyte in a sample and kits containing same are also disclosed.
摘要翻译： 公开了由自组装肽组成的涂有肽纳米结构的电极。 电极能够传导由电化学反应产生的响应电流。 电极可以形成电化学电池的一部分，检测器和传感器阵列。 还公开了利用电化学电池，检测器或传感器阵列的方法，其包括用于检测样品中的分析物的电极和包含其的试剂盒。
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公开(公告)号：US5082550A
公开(公告)日：1992-01-21
申请号：US448475
申请日：1989-12-11
IPC分类号： G01N27/327 , C12Q1/00
CPC分类号： C12Q1/002 , Y10S435/817
摘要： An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.
摘要翻译： 电化学传感器电极由涂覆有含有全氟磺酸离聚物和选定酶的流延溶液的电子导体形成。 所选择的酶催化溶液中的预定物质与氧之间的反应，以形成在电子导体处检测到的电化学活性化合物。 所得的全氟磺酸聚合物为长寿命酶活性的酶提供稳定的基质，其中在金属导体上仅需要薄涂层。 该聚合物还有利地排除干扰物质接触酶，并含有一定量的氧以在样品中的氧耗尽条件下保持感测能力。 在一个具体实施方案中，葡萄糖氧化酶与全氟磺酸离聚物混合以形成用于葡萄糖检测的电极。
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公开(公告)号：US09316592B2
公开(公告)日：2016-04-19
申请号：US13487205
申请日：2012-06-03
IPC分类号： G01N33/551 , G01N21/77
CPC分类号： G01N21/7703 , G01N2021/7779
摘要： Light-emitting intra-cavity interferometric (ICI) optical sensors based on channel waveguide structures which include an internal light emitting material and a functionalized region. In some embodiments, the waveguides are made of a sol-gel which incorporates the light emitting material. In some embodiments, the waveguide structure includes an ICI resonator backbone and the ICI sensor is a laser sensor. In some embodiments, the resonator backbone has an interferometric Y-branch shape. In some embodiments, the resonator backbone has a Mach Zehnder interferometer shape. In some embodiments, an ICI laser sensor has an interferometric arrayed waveguide grating shape. In some embodiments, an ICI sensor may be remotely optically pumped and remotely read.
摘要翻译： 基于包括内部发光材料和官能化区域的通道波导结构的发光腔内干涉（ICI）光学传感器。 在一些实施例中，波导由掺入发光材料的溶胶 - 凝胶制成。 在一些实施例中，波导结构包括ICI谐振器主干，ICI传感器是激光传感器。 在一些实施例中，谐振器主干具有干涉Y分支形状。 在一些实施例中，谐振器主干具有马赫曾德干涉仪形状。 在一些实施例中，ICI激光传感器具有干涉阵列波导光栅形状。 在一些实施例中，ICI传感器可以被远程光泵浦和远程读取。
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