公开(公告)号：US20120308615A1

公开(公告)日：2012-12-06

申请号：US13498842

申请日：2010-09-28

申请人： Julio César Aguilar Rubido ,  Yadira Lobaina Mato ,  Dunia Hernández Inguanzo ,  Heidy Trujillo Pérez ,  Freya de los Milagros Freyre Almeida ,  Verena Lucila Muzio González ,  Eduardo Pénton Arias ,  Sonia Gonzalez Blanco ,  Raimundo Ubieta Gómez ,  Gerardo Enrique Guillén Nieto ,  Luis Saturnino Herrera Martinez

发明人： Julio César Aguilar Rubido ,  Yadira Lobaina Mato ,  Dunia Hernández Inguanzo ,  Heidy Trujillo Pérez ,  Freya de los Milagros Freyre Almeida ,  Verena Lucila Muzio González ,  Eduardo Pénton Arias ,  Sonia Gonzalez Blanco ,  Raimundo Ubieta Gómez ,  Gerardo Enrique Guillén Nieto ,  Luis Saturnino Herrera Martinez

IPC分类号： A61K39/29 ,  A61P31/14 ,  C12N5/0781 ,  C12N5/0786 ,  C12N5/0784 ,  A61K9/14 ,  C12N5/078

CPC分类号： A61K9/145 ,  A61K9/10 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/5158 ,  A61K2039/54 ,  A61K2039/543 ,  A61K2039/545 ,  A61K2039/55505 ,  C12N2730/10134

摘要： The present invention is related with the field of the therapeutic immunization, specifically with the employment of a new formulation of antigens of the Hepatitis B Virus (HBV) for the cellular stimulation. The formulation is formed by the surface antigens (HBsAg) precipitated in suspension and the nucleocapsid (HBcAg) of the HBV. The formulation contains these antigens like suspended particles of sizes less than 500 nm and higher than 500 nm, in a mixture where the proportion among the particles of the mentioned sizes is among 50%-50% and 80%-20%, respectively. The selection of a range of sizes of particles allows that the levels of stimulation of several cellular types are maximized. Additionally, a cellular stimulation method is described with this formulation, and the later passive immunization of patients with chronic Hepatitis B, based on the maximum stimulation in vivo or in vitro using heterologous or autologous cells (dendritic cells, B cells and macrophages). The cells stimulated with this formulation are transferred to patients chronically infected with the HBV.

摘要翻译： 本发明涉及治疗性免疫领域，特别是使用乙型肝炎病毒（HBV）抗原的新配方用于细胞刺激。 该制剂由悬浮液中沉淀的表面抗原（HBsAg）和HBV的核衣壳（HBcAg）形成。 在上述尺寸的颗粒中的比例分别为50％-50％至80％-20％的混合物中，该制剂含有这些抗原，如尺寸小于500nm且高于500nm的悬浮颗粒。 颗粒尺寸范围的选择使得几种细胞类型的刺激水平最大化。 另外，根据体内或体外使用异源或自体细胞（树突状细胞，B细胞和巨噬细胞）的最大刺激，用该配方描述细胞刺激方法，以及慢性乙型肝炎患者随后的被动免疫。 用该制剂刺激的细胞转移到长期感染HBV的患者。