公开(公告)号：US10351890B2

公开(公告)日：2019-07-16

申请号：US15533273

申请日：2015-12-09

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Hannah Chung

IPC: C12P21/00 ,  A61K39/00 ,  C12N15/113

Abstract: The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

公开(公告)号：US20180327801A1

公开(公告)日：2018-11-15

申请号：US16027478

申请日：2018-07-05

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Hannah Chung

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/50 ,  C12Y301/26005

Abstract: A method is described for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. By the disclosed method, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

公开(公告)号：US20170342454A1

公开(公告)日：2017-11-30

申请号：US15533273

申请日：2015-12-09

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Hannah Chung

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/50 ,  C12Y301/26005

Abstract: The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.