公开(公告)号：US20240355419A1

公开(公告)日：2024-10-24

申请号：US18640635

申请日：2024-04-19

申请人： UT-Battelle, LLC

发明人： Xiaohan Yang ,  Md Mahmudul Hassan ,  Stanton Martin ,  Gerald A. Tuskan ,  Guoliang Yuan

IPC分类号： G16B25/20 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  G16B35/20

CPC分类号： G16B25/20 ,  C12N9/22 ,  C12N15/1096 ,  C12N15/111 ,  G16B35/20 ,  C12N2310/121 ,  C12N2310/123 ,  C12N2310/20 ,  C12Y301/26005 ,  C12Y301/26011

摘要： The present disclosure is directed to polycistronic guide RNAs, DNA encoding polycistronic gRNA, multiplex CRISPR vectors, a plurality of component DNA fragments for assembly into a DNA encoding a polycistronic gRNA array, a plurality of primer pairs for making a plurality of component DNA fragments to be assembled into a DNA encoding a polycistronic gRNA, and methods of making multiplex CRISPR vectors. The current disclosure is directed to multiplexed CRISPR technologies that have great potential for pathway engineering and genome editing. In the current disclosure describes efficient assembly of tRNA/Csy4/Ribozyme-based gRNA arrays which can be produced in a quick and effective process.

公开(公告)号：US11920174B2

公开(公告)日：2024-03-05

申请号：US16081863

申请日：2017-03-03

申请人： CureVac SE

发明人： Fabian Johannes Eber ,  Aniela Wochner ,  Tilmann Roos

IPC分类号： C12P19/34 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6869 ,  G16B30/00

CPC分类号： C12P19/34 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/6809 ,  G16B30/00 ,  C12Q1/6869 ,  C12Y301/03001 ,  C12Y301/04001 ,  C12Y301/26005 ,  C12Q1/6809 ,  C12Q2521/327 ,  C12Q2525/117 ,  C12Q2525/173 ,  C12Q2525/204 ,  C12Q2527/125 ,  C12Q2565/137 ,  C12Q1/6806 ,  C12Q2521/327 ,  C12Q2525/117 ,  C12Q2525/173 ,  C12Q2525/204 ,  C12Q2527/125 ,  C12Q2565/137

摘要： The present invention relates to analysis of an RNA molecule. It further relates to the use of this method for the quality control of an RNA molecule produced by in vitro transcription or for the quality control of an RNA molecule produced by chemical synthesis.

公开(公告)号：US20170199187A1

公开(公告)日：2017-07-13

申请号：US15321491

申请日：2015-06-23

申请人： INOVA DIAGNOSTICS, INC.

发明人： Michael MAHLER

IPC分类号： G01N33/564

CPC分类号： G01N33/564 ,  C07K2319/40 ,  C12N9/22 ,  C12Y301/26005 ,  G01N2800/101

摘要： The present disclosure relates to the field of molecular biology and immunology. More specifically, the present disclosure provides compositions and methods for detecting anti-Th/To autoantibodies in the serum of subject with a systemic autoimmune disease, such as systemic sclerosis (SSc).

公开(公告)号：US20230250494A1

公开(公告)日：2023-08-10

申请号：US17560573

申请日：2021-12-23

申请人： Reditus Laboratories, LLC

发明人： Joshua J. Geltz

IPC分类号： C12Q1/70 ,  C12Q1/686 ,  C12Q1/6876 ,  C12N9/22

CPC分类号： C12Q1/701 ,  C12Q1/686 ,  C12Q1/6876 ,  C12N9/22 ,  C12Y301/26005 ,  C12Q2600/16

摘要： A composition for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR) is provided. Also provided is a kit comprising the above composition with additional reagents for performing PCR using the plasmid, dye, primers and probes to detect the more than one target nucleic acid sequence. Additionally provided is a method for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR).

公开(公告)号：US20180327801A1

公开(公告)日：2018-11-15

申请号：US16027478

申请日：2018-07-05

申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Sang Yup Lee ,  Hannah Chung

IPC分类号： C12P21/00 ,  C12N15/113

CPC分类号： C12P21/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/50 ,  C12Y301/26005

摘要： A method is described for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. By the disclosed method, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

公开(公告)号：US20170342454A1

公开(公告)日：2017-11-30

申请号：US15533273

申请日：2015-12-09

申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Sang Yup Lee ,  Hannah Chung

IPC分类号： C12P21/00 ,  C12N15/113

CPC分类号： C12P21/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/50 ,  C12Y301/26005

摘要： The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.