公开(公告)号：US11320442B2

公开(公告)日：2022-05-03

申请号：US15491184

申请日：2017-04-19

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Min Cheol Park ,  Youhee Heo

IPC: G01N33/68 ,  C12N13/00

Abstract: Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.

公开(公告)号：US10648988B2

公开(公告)日：2020-05-12

申请号：US15878351

申请日：2018-01-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Youhee Heo ,  Kyoseon Hwang ,  Myung-Sic Chae

IPC: G01N33/53 ,  G01N25/18 ,  G01N24/00 ,  G01N27/00 ,  G01N33/68 ,  A61B5/145 ,  A61B5/1468 ,  A61B5/00 ,  A61B5/1486 ,  C12Q1/6883

Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.