公开(公告)号：US10648988B2

公开(公告)日：2020-05-12

申请号：US15878351

申请日：2018-01-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Youhee Heo ,  Kyoseon Hwang ,  Myung-Sic Chae

IPC: G01N33/53 ,  G01N25/18 ,  G01N24/00 ,  G01N27/00 ,  G01N33/68 ,  A61B5/145 ,  A61B5/1468 ,  A61B5/00 ,  A61B5/1486 ,  C12Q1/6883

Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.

公开(公告)号：US09061137B2

公开(公告)日：2015-06-23

申请号：US13688729

申请日：2012-11-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo Hyun Lee ,  Ji Yoon Kang ,  Nakwon Choi

IPC: A61N1/05 ,  A61L31/16 ,  A61L31/10 ,  A61B5/04

CPC classification number: A61N1/0556 ,  A61B5/04001 ,  A61B2562/0209 ,  A61B2562/125 ,  A61L31/10 ,  A61L31/16 ,  A61L2300/41 ,  A61L2300/43 ,  A61L2400/12 ,  A61L2420/08 ,  A61N1/0553

Abstract: A nerve electrode that is inserted into a living body and that is configured to attach to nerves is provided. The nerve electrode that is inserted into a living body and that is configured to attach to nerves includes: i) a flexible substrate; ii) a plurality of electrodes that are separately positioned on the flexible substrate; and iii) an insulating layer that is positioned at a separation space of the plurality of electrodes and that insulates the plurality of electrodes. The plurality of electrodes include i) at least one linear electrode, and ii) a planar electrode that is separated from the linear electrode. An anti-inflammatory drug transfer layer is positioned on the planar electrode.

Abstract translation: 提供了插入生物体并被配置为附着于神经的神经电极。 插入到生物体中并被配置为附着到神经的神经电极包括：i）柔性基底; ii）分别定位在柔性基板上的多个电极; 以及iii）绝缘层，其位于所述多个电极的分离空间处并使所述多个电极绝缘。 多个电极包括i）至少一个线状电极，以及ii）与线状电极分离的平面电极。 将抗炎药物转移层定位在平面电极上。

公开(公告)号：US11668709B2

公开(公告)日：2023-06-06

申请号：US16579852

申请日：2019-09-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo Hyun Lee ,  Ji Yoon Kang ,  Hyewhon Rhim ,  Yi Jae Lee ,  Hyewon Seo ,  Yun Kyung Kim

IPC: G01N33/543 ,  G01N33/68 ,  G16B40/10

CPC classification number: G01N33/54373 ,  G01N33/54326 ,  G01N33/6845 ,  G01N33/6896 ,  G16B40/10 ,  G01N2440/00 ,  G01N2440/14 ,  G01N2440/38 ,  G01N2800/2821

Abstract: The present disclosure relates to a system for monitoring post-translational modification of protein using a biosensor with a gap, which performs with high reliability a diagnosis of a disease associated with a target protein for which impedance is measured, by measuring an impedance of a sample introduced into a sensor and calculating a change rate of the measured impedance, and to a method of manufacturing the biosensor used for the system.

公开(公告)号：US11313828B2

公开(公告)日：2022-04-26

申请号：US14994389

申请日：2016-01-13

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Kyoseon Hwang ,  Tae Song Kim

IPC: G01N27/416 ,  G01N27/327 ,  G01N33/543

Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.

公开(公告)号：US10585105B2

公开(公告)日：2020-03-10

申请号：US15565503

申请日：2016-05-18

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong Sik Shin ,  Jae Hoon Ji ,  Tae Song Kim ,  Young Soo Kim ,  Sang Yun Kim

IPC: G01N33/53 ,  G01N25/18 ,  G01N24/00 ,  G01N27/00 ,  G01N33/68 ,  G01N27/02

Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.

公开(公告)号：US10585022B2

公开(公告)日：2020-03-10

申请号：US15216938

申请日：2016-07-22

Applicant: Korea Institute of Science and Technology

Inventor： Rhokyun Kwak ,  Ji Yoon Kang ,  Jaesung Park ,  Siwoo Cho

IPC: C12Q1/68 ,  G01N1/40 ,  G01N27/447 ,  C12Q1/6806 ,  C12Q1/00

Abstract: Provided are an extracellular vesicle collecting apparatus using an electrode and a porous membrane and a method for using the same. The extracellular vesicle collecting apparatus includes i) an upper frame in which a pair of through holes is disposed so that a buffer solution flows therethrough, ii) a buffer tube which is inserted in the through holes and in which the buffer solution flows, iii) a positive electrode disposed on the upper frame, iv) a porous membrane located below the upper frame, v) a spacer which is located below the porous membrane and has a hollow space disposed therein, vi) a plurality of guide tubes through which a blood flows in the hollow space, vii) a lower frame which is coupled to the upper frame to be opposite to the upper frame and receives the porous membrane and the spacer therein, and viii) a negative electrode provided below the lower frame. Extracellular vesicles contained in the blood are collected by the porous membrane.

公开(公告)号：US11320442B2

公开(公告)日：2022-05-03

申请号：US15491184

申请日：2017-04-19

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Min Cheol Park ,  Youhee Heo

IPC: G01N33/68 ,  C12N13/00

Abstract: Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.

公开(公告)号：US20170131233A1

公开(公告)日：2017-05-11

申请号：US14994389

申请日：2016-01-13

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Kyoseon Hwang ,  Tae Song Kim

IPC: G01N27/416 ,  G01N33/68 ,  G01N27/327

CPC classification number: G01N27/4166 ,  G01N27/3275 ,  G01N27/745 ,  G01N33/5438

Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.

公开(公告)号：US20140100639A1

公开(公告)日：2014-04-10

申请号：US13688729

申请日：2012-11-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo Hyun Lee ,  Ji Yoon Kang ,  Nakwon Choi

IPC: A61N1/05

CPC classification number: A61N1/0556 ,  A61B5/04001 ,  A61B2562/0209 ,  A61B2562/125 ,  A61L31/10 ,  A61L31/16 ,  A61L2300/41 ,  A61L2300/43 ,  A61L2400/12 ,  A61L2420/08 ,  A61N1/0553

Abstract: A nerve electrode that is inserted into a living body and that is configured to attach to nerves is provided. The nerve electrode that is inserted into a living body and that is configured to attach to nerves includes: i) a flexible substrate; ii) a plurality of electrodes that are separately positioned on the flexible substrate; and iii) an insulating layer that is positioned at a separation space of the plurality of electrodes and that insulates the plurality of electrodes. The plurality of electrodes include i) at least one linear electrode, and ii) a planar electrode that is separated from the linear electrode. An anti-inflammatory drug transfer layer is positioned on the planar electrode.

Abstract translation: 提供了插入生物体并被配置为附着于神经的神经电极。 插入到生物体中并被配置为附着到神经的神经电极包括：i）柔性基底; ii）分别定位在柔性基板上的多个电极; 以及iii）绝缘层，其位于所述多个电极的分离空间处并使所述多个电极绝缘。 多个电极包括i）至少一个线状电极，以及ii）与线状电极分离的平面电极。 将抗炎药物转移层定位在平面电极上。