公开(公告)号：US20150299665A1

公开(公告)日：2015-10-22

申请号：US14340991

申请日：2014-07-25

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Yee Sook Cho ,  Chae Wha Yoo ,  Hee Jun Na

IPC: C12N5/074 ,  A61K35/12 ,  C12N5/071

CPC classification number: C12N5/0696 ,  A61K35/32 ,  A61K35/44 ,  C12N5/069 ,  C12N5/0691 ,  C12N5/0692 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2506/1361 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/90

Abstract: The present invention relates to a method for producing endothelial progenitor cells, comprising a method for producing induced pluripotent stem cells by using only Oct4 and Sox2 as a reprogramming factor of human dental pulp cells, and differentiating endothelial progenitor cells from the induced pluripotent stem cells produced by the method for producing induced pluripotent stem cells. Moreover, the present invention relates to a method for producing endothelial cells, comprising differentiating endothelial cells from endothelial progenitor cells produced by the method for producing the endothelial progenitor cells. Additionally, the present invention relates to a method for producing smooth muscle cells, comprising differentiating smooth muscle cells from endothelial progenitor cells produced by the method for producing the endothelial progenitor cells.Also, the present invention make a significant contribution to treatment of ischemia disease and heart disease because a patient's dental pulp cell-derived differentiated cells can be used for cell treatment as a result of the cell treatment effect of the endothelial progenitor cells in a mouse model induced to have hind limb ischemia or myocardial infarction.

Abstract translation: 本发明涉及一种生产内皮祖细胞的方法，其包括通过仅使用Oct4和Sox2作为人牙髓细胞的重新编程因子来产生诱导的多能干细胞的方法，以及从产生的诱导多能干细胞分化内皮祖细胞 通过产生诱导多能干细胞的方法。 此外，本发明涉及一种生产内皮细胞的方法，其包括将内皮细胞与由内皮祖细胞产生的方法产生的内皮祖细胞分化。 此外，本发明涉及一种平滑肌细胞的制备方法，其包括通过用于产生内皮祖细胞的方法产生的内皮祖细胞分化平滑肌细胞。 此外，本发明对于缺血性疾病和心脏病的治疗有显着的贡献，因为患者的牙髓细胞衍生的分化细胞可以作为小鼠模型中内皮祖细胞的细胞处理效果的细胞治疗而使用 诱发后肢缺血或心肌梗死。