公开(公告)号：US20150299733A1

公开(公告)日：2015-10-22

申请号：US14111721

申请日：2012-04-13

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Jung Hoon Sohn ,  Hyun Jin Kim ,  Chul Ho Kim ,  Ji Hun Seomoon ,  Bong Hyun Sung ,  Kwang Mook Lim ,  Mi Jin Kim ,  Jong Hyun Kim

IPC: C12P7/06 ,  C12N9/42

CPC classification number: C12P7/06 ,  C12N9/2445 ,  C12Y302/01021 ,  Y02E50/17

Abstract: The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.

Abstract translation: 本发明涉及突变以提高活性的β-葡糖苷酶，以及使用其生产生物乙醇的方法。 更具体地说，本发明涉及一种突变为具有增强活性的β-葡糖苷酶的多核苷酸，由多核苷酸表达的β-葡糖苷酶，包含多核苷酸的表达载体，用表达载体转化的转化体， 使用转化体产生突变的β-葡糖苷酶，以及使用转化体生产生物乙醇的方法。 本发明的突变型β-葡糖苷酶比常规的β-葡糖苷酶增加葡萄糖的产量，因此可广泛用于生物乙醇的经济生产。