公开(公告)号：US20150299733A1

公开(公告)日：2015-10-22

申请号：US14111721

申请日：2012-04-13

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Jung Hoon Sohn ,  Hyun Jin Kim ,  Chul Ho Kim ,  Ji Hun Seomoon ,  Bong Hyun Sung ,  Kwang Mook Lim ,  Mi Jin Kim ,  Jong Hyun Kim

IPC: C12P7/06 ,  C12N9/42

CPC classification number: C12P7/06 ,  C12N9/2445 ,  C12Y302/01021 ,  Y02E50/17

Abstract: The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.

Abstract translation: 本发明涉及突变以提高活性的β-葡糖苷酶，以及使用其生产生物乙醇的方法。 更具体地说，本发明涉及一种突变为具有增强活性的β-葡糖苷酶的多核苷酸，由多核苷酸表达的β-葡糖苷酶，包含多核苷酸的表达载体，用表达载体转化的转化体， 使用转化体产生突变的β-葡糖苷酶，以及使用转化体生产生物乙醇的方法。 本发明的突变型β-葡糖苷酶比常规的β-葡糖苷酶增加葡萄糖的产量，因此可广泛用于生物乙醇的经济生产。

公开(公告)号：US08986996B2

公开(公告)日：2015-03-24

申请号：US13669896

申请日：2012-11-06

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Yee Sook Cho ,  Mi Young Son ,  Hyun Jin Kim

IPC: C12N5/071 ,  C12N5/0735 ,  C12N5/073 ,  C12M1/32 ,  C12M1/12 ,  C12M1/33 ,  C12M1/00

CPC classification number: C12N5/0606 ,  C12M23/12 ,  C12M25/06 ,  C12M45/02 ,  C12M45/09 ,  C12N5/0603 ,  C12N2509/10

Abstract: The present invention relates to a large-scale propagation and maintenance method of embryoid bodies generated from stem cells. More particularly, the present invention relates to a large-scale propagation and maintenance method of embryoid bodies retaining their intrinsic characteristics for a long period of time, comprising the step of continuously subculturing embryoid bodies that are primarily produced from embryonic stem cells or from induced pluripotent stem cells. According to the method of the present invention, after preparation of embryoid bodies from a limited number of stem cells, large-scale production and maintenance of embryoid bodies can be realized by a simple mechanical subculturing method without the continuous supply of stem cells.

Abstract translation: 本发明涉及从干细胞产生的胚状体的大规模繁殖和维持方法。 更具体地说，本发明涉及长期保持其固有特征的胚状体的大规模繁殖和维持方法，包括连续传代培养主要由胚胎干细胞或诱导多能的胚状体的步骤 干细胞。 根据本发明的方法，在从有限数量的干细胞制备胚状体后，通过简单的机械传代培养方法可以实现胚状体的大规模生产和维持，而不需要连续供应干细胞。