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    Mutant Beta-Glucosidases Having Enhanced Activity and a Method for Producing Bioethanol Using the Same
    4.
    发明申请
    Mutant Beta-Glucosidases Having Enhanced Activity and a Method for Producing Bioethanol Using the Same 有权
    具有增强活性的突变型β-葡糖苷酶和使用其生产生物乙醇的方法

    公开(公告)号：US20150299733A1

    公开(公告)日：2015-10-22

    申请号：US14111721

    申请日：2012-04-13

    Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

    Inventor： Jung Hoon Sohn ,  Hyun Jin Kim ,  Chul Ho Kim ,  Ji Hun Seomoon ,  Bong Hyun Sung ,  Kwang Mook Lim ,  Mi Jin Kim ,  Jong Hyun Kim

    IPC: C12P7/06 ,  C12N9/42

    CPC classification number: C12P7/06 ,  C12N9/2445 ,  C12Y302/01021 ,  Y02E50/17

    Abstract: The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.

    Abstract translation: 本发明涉及突变以提高活性的β-葡糖苷酶，以及使用其生产生物乙醇的方法。 更具体地说，本发明涉及一种突变为具有增强活性的β-葡糖苷酶的多核苷酸，由多核苷酸表达的β-葡糖苷酶，包含多核苷酸的表达载体，用表达载体转化的转化体， 使用转化体产生突变的β-葡糖苷酶，以及使用转化体生产生物乙醇的方法。 本发明的突变型β-葡糖苷酶比常规的β-葡糖苷酶增加葡萄糖的产量，因此可广泛用于生物乙醇的经济生产。

    METHOD FOR PRODUCING 1,3-PROPANEDIOL USING MICROORGANISM VARIANT WITH DELETION OF 2,3-BUTANEDIOL SYNTHETIC GENE
    5.
    发明申请
    METHOD FOR PRODUCING 1,3-PROPANEDIOL USING MICROORGANISM VARIANT WITH DELETION OF 2,3-BUTANEDIOL SYNTHETIC GENE 有权
    用去除2,3-丁二醇合成基因生产具有微生物变体的1,3-丙二醇的方法

    公开(公告)号：US20170016032A1

    公开(公告)日：2017-01-19

    申请号：US15120516

    申请日：2015-02-16

    Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

    Inventor： Chul Ho Kim ,  Jeong-Woo Seo ,  Sun-Yeon Heo ,  Sung Mok Lee ,  Jang Min Park ,  Baek Rock Oh ,  You Ree Jung

    IPC: C12P7/18 ,  C12N9/88 ,  C12N9/10

    CPC classification number: C12P7/18 ,  C12N1/00 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/93 ,  C12Y202/01006 ,  C12Y401/01001

    Abstract: The present invention relates to a method for producing 1,3-propanediol using a mutant microorganism lacking a 2,3-butanediol synthetic gene, and more particularly to a mutant microorganism wherein a gene encoding lactate dehydrogenase and a gene encoding an enzyme which is involved in 2,3-butanediol synthesis are deleted in a microorganism having the ability to produce 1,3-propanediol from glycerol and wherein a gene encoding pyruvate decarboxylase and a gene encoding aldehyde dehydrogenase are introduced or amplified, and to a method of promoting the production of 1,3-propanediol while inhibiting the production of 2,3-butanediol by using the mutant microorganism. The use of the glycerol-fermenting mutant microorganism according to the present invention can significantly increase the production of 1,3-propanediol while minimizing the production of 2,3-butanediol.

    Abstract translation: 本发明涉及一种使用缺少2,3-丁二醇合成基因的突变型微生物生产1,3-丙二醇的方法，特别涉及编码乳酸脱氢酶的基因和编码涉及酶的基因的突变型微生物 在2,3-丁二醇合成中，在具有从丙三醇生产1,3-丙二醇的能力的微生物中缺失，其中引入或扩增编码丙酮酸脱羧酶的基因和编码醛脱氢酶的基因，以及促进生产的方法 的1,3-丙二醇，同时通过使用突变微生物抑制2,3-丁二醇的产生。 使用根据本发明的甘油发酵突变体微生物可以显着增加1,3-丙二醇的产量，同时使2,3-丁二醇的生产最小化。

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