公开(公告)号：US20240402184A1

公开(公告)日：2024-12-05

申请号：US18666454

申请日：2024-05-16

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Eun Kyung LIM ,  Ji Sun Ki ,  Jaewoo Lim ,  Taejoon Kang ,  Juyeon Jung

IPC: G01N33/58 ,  C12N9/22 ,  C12Q1/44 ,  G01N33/52 ,  G01N33/543 ,  G01N33/569

Abstract: The present disclosure relates to a dual enzyme amplification-based colorimetric sensor system for on-site detection of pathogens. The colorimetric sensor system according to the present disclosure may comprise a combination of the CRISPR/Cas12a system with an enzymatic reaction of urease, thereby facilitating on-site detection of pathogens without separate analytical equipment by analyzing the color change through dual enzyme amplification. In addition, it is possible to selectively change the target by changing the crRNA sequence depending on the target pathogen to be detected, which has the advantage of being applicable to various types of pathogens without limitation. Further, the present disclosure can be used as a point of care service (PoC) system capable of detecting the genes of pathogens directly down to the sub-ng level without separate analysis equipment by applying the detection color value derived using the colorimetric sensor system according to the present disclosure to a smartphone application (app).