公开(公告)号：US20160160183A1

公开(公告)日：2016-06-09

申请号：US14906547

申请日：2014-07-22

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Sungoi Hong ,  Hang-Soo Park ,  In-Sik Hwang ,  Kyung-A Choi

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727

Abstract: The present invention relates to a composition for maintaining the chromosomal stability of pluripotent stem cells, which contains a small-molecule compound, and more particularly, to a composition for maintaining the chromosomal stability of induced pluripotent stem cells, which contains a ROCK inhibitor, a MEK inhibitor, an ALK5 inhibitor or an antioxidant, which is capable of inhibiting chromosomal structural and numerical mutations or DNA damage during induction of induced pluripotent stem cell and the subsequent culture of the cells. The small-molecule compound-containing composition for maintaining the chromosomal stability of induced pluripotent stem cells increases the reprogramming rate and efficiency during production of induced pluripotent stem cells and exhibits excellent effects on the inhibition of chromosomal structural and numerical mutations or DNA damage. Thus, the composition of the present invention is very useful for the development of induced pluripotent stem cell therapeutic agents having excellent chromosomal stability.

Abstract translation: 本发明涉及用于维持含有小分子化合物的多能干细胞的染色体稳定性的组合物，更具体地，涉及用于维持诱导多能干细胞的染色体稳定性的组合物，其含有ROCK抑制剂， MEK抑制剂，ALK5抑制剂或抗氧化剂，其能够在诱导的多能干细胞诱导期间抑制染色体结构和数值突变或DNA损伤，并随后培养细胞。 用于维持诱导多能干细胞染色体稳定性的小分子化合物组合物在诱导多能干细胞的产生过程中增加了重编程速率和效率，并且对抑制染色体结构和数值突变或DNA损伤表现出极好的效果。 因此，本发明的组合物对于具有优异的染色体稳定性的诱导多能干细胞治疗剂的开发非常有用。

公开(公告)号：US09752127B2

公开(公告)日：2017-09-05

申请号：US14906547

申请日：2014-07-22

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Sunghoi Hong ,  Hang-Soo Park ,  In-Sik Hwang ,  Kyung-A Choi

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727

Abstract: The present invention relates to a composition for maintaining the chromosomal stability of pluripotent stem cells, which contains a small-molecule compound, and more particularly, to a composition for maintaining the chromosomal stability of induced pluripotent stem cells, which contains a ROCK inhibitor, a MEK inhibitor, an ALK5 inhibitor or an antioxidant, which is capable of inhibiting chromosomal structural and numerical mutations or DNA damage during induction of induced pluripotent stem cell and the subsequent culture of the cells. The small-molecule compound-containing composition for maintaining the chromosomal stability of induced pluripotent stem cells increases the reprogramming rate and efficiency during production of induced pluripotent stem cells and exhibits excellent effects on the inhibition of chromosomal structural and numerical mutations or DNA damage. Thus, the composition of the present invention is very useful for the development of induced pluripotent stem cell therapeutic agents having excellent chromosomal stability.

公开(公告)号：US09540614B2

公开(公告)日：2017-01-10

申请号：US14222697

申请日：2014-03-24

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Sunghoi Hong ,  Seung-Ick Oh ,  Hang-Soo Park

IPC: C12N5/095

CPC classification number: C12N5/0695 ,  C12N2502/1358 ,  C12N2506/25 ,  C12N2510/04

Abstract: A method is described for generating a novel cancer stem cell line that possesses characteristics associated with stem cells, by co-culturing a human immortalized cell line and bone marrow-derived mesenchymal stem cells, and the novel cancer stem cell line established thereby. This method is able to readily generate cancer stem cells that are low in the level of structural chromosomal aberrations and are excellent in oncogenicity, and is effectively applicable to the development of anti-cancer drugs and personalized drugs.

Abstract translation: 描述了通过共培养人类永生化细胞系和骨髓间充质干细胞以及由此建立的新型癌干细胞系来产生具有与干细胞相关特征的新型癌干细胞系的方法。 该方法能够容易地生成结构染色体畸变水平低的癌干细胞，致癌性优异，有效适用于抗癌药物和个性化药物的开发。

公开(公告)号：US20150104864A1

公开(公告)日：2015-04-16

申请号：US14222697

申请日：2014-03-24

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Sunghoi Hong ,  Seung-Ick Oh ,  Hang-Soo Park

IPC: C12N5/095

CPC classification number: C12N5/0695 ,  C12N2502/1358 ,  C12N2506/25 ,  C12N2510/04

Abstract: A method is described for generating a novel cancer stem cell line that possesses characteristics associated with stem cells, by co-culturing a human immortalized cell line and bone marrow-derived mesenchymal stem cells, and the novel cancer stem cell line established thereby. This method is able to readily generate cancer stem cells that are low in the level of structural chromosomal aberrations and are excellent in oncogenicity, and is effectively applicable to the development of anti-cancer drugs and personalized drugs.

Abstract translation: 描述了通过共培养人类永生化细胞系和骨髓间充质干细胞以及由此建立的新型癌干细胞系来产生具有与干细胞相关特征的新型癌干细胞系的方法。 该方法能够容易地生成结构染色体畸变水平低的癌干细胞，致癌性优异，有效适用于抗癌药物和个性化药物的开发。