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    METHOD OF PRODUCING ANTIBODIES FROM SINGLE PLASMA CELLS
    1.
    发明公开
    METHOD OF PRODUCING ANTIBODIES FROM SINGLE PLASMA CELLS 审中-公开

    公开(公告)号：US20240150446A1

    公开(公告)日：2024-05-09

    申请号：US18505543

    申请日：2023-11-09

    Applicant: Daro Zist Azma Oxin

    Inventor： Zohre BAZAZ ,  Masoume BAZAZ

    IPC: C07K16/12 ,  C12N5/0781

    CPC classification number: C07K16/1282 ,  C12N5/0635 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/622 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2501/999 ,  C12N2502/1358

    Abstract: A method for producing an antibody fragment from one or more single plasma cells. The method comprises obtaining a plurality of plasma cells, preparing a plurality of modified cell culture substrates, seeding each respective plasma cell of the plurality of plasma cells on the each respective modified cell culture substrate of the plurality of modified cell culture substrates, forming a plurality of cultured single plasma cells by culturing the seeded each respective plasma cell in a culture medium for a time duration of at least 10 days, and detecting the one or more single plasma cells that secrete the antibody fragment from among the plurality of cultured single plasma cells. The culture medium comprises a conditioned medium harvested from a culture of h-BMSCs, Insulin, Transferrin, Selenium, and Pyruvate.

    Method for Treating Diabetes and Other Glucose Regulation Disorders Using Stem Cells
    6.
    发明申请
    Method for Treating Diabetes and Other Glucose Regulation Disorders Using Stem Cells 审中-公开

    公开(公告)号：US20170327794A1

    公开(公告)日：2017-11-16

    申请号：US15442991

    申请日：2017-02-27

    Applicant: InCube Labs, LLC

    Inventor： Mir Imran

    IPC: C12N5/071 ,  A61K35/38

    CPC classification number: A61K35/38 ,  A61K35/28 ,  C12N5/0679 ,  C12N2502/025 ,  C12N2502/03 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/23 ,  C12N2506/1346 ,  C12N2506/03

    Abstract: Various embodiments of the invention provide methods of treating diabetes and other glucose regulation disorders. In one embodiment, the method comprises removing L-cells from a donor, obtaining stem cells from a patient, and culturing the L-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived L-cells (SCDLC). An amount of the SCDLC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food. In another embodiment, the method comprises removing K-cells from a donor, obtaining stem cells from a patient, and culturing the K-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived K-cells (SCDKC). An amount of the SCDKC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food.

    Method for treating diabetes and other glucose regulation disorders using stem cells
    8.
    发明授权
    Method for treating diabetes and other glucose regulation disorders using stem cells 有权

    公开(公告)号：US09617518B2

    公开(公告)日：2017-04-11

    申请号：US14833403

    申请日：2015-08-24

    Applicant: Incube Labs, LLC

    Inventor： Mir Imran

    IPC: C12N5/077 ,  A61K35/38 ,  C12N5/071

    CPC classification number: C12N5/0679 ,  A61K35/38 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/23 ,  C12N2506/1346 ,  C12N2506/1353

    Abstract: Various embodiments of the invention provide methods of treating diabetes and other glucose regulation disorders. In one embodiment, the method comprises removing L-cells from a donor, obtaining stem cells from a patient, and culturing the L-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived L-cells (SCDLC). An amount of the SCDLC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food. In another embodiment, the method comprises removing K-cells from a donor, obtaining stem cells from a patient, and culturing the K-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived K-cells (SCDKC). An amount of the SCDKC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food.

    NEO-ISLETS COMPRISING STEM AND ISLET CELLS AND TREATMENT OF DIABETES MELLITUS THEREWITH
    9.
    发明申请
    NEO-ISLETS COMPRISING STEM AND ISLET CELLS AND TREATMENT OF DIABETES MELLITUS THEREWITH 审中-公开
    包含干细胞和分离细胞的新近分离物及其治疗糖尿病

    公开(公告)号：US20170073641A1

    公开(公告)日：2017-03-16

    申请号：US15261750

    申请日：2016-09-09

    Applicant: SymbioCellTech, LLC

    Inventor： Christof Westenfelder ,  Anna Gooch ,  Ping Zhang ,  Zhuma Hu

    IPC: C12N5/071 ,  A61K35/39 ,  A61K35/35 ,  A61K9/00 ,  A61K31/436 ,  C12N5/00 ,  A61K45/06

    CPC classification number: C12N5/0676 ,  A61K9/0019 ,  A61K31/436 ,  A61K35/35 ,  A61K35/39 ,  A61K45/06 ,  C12N5/0012 ,  C12N5/0677 ,  C12N2502/1358 ,  C12N2502/1382

    Abstract: Described are Neo-Islets comprising: a) dedifferentiated islet cells and mesenchymal and/or adipose stem cells; or b) redifferentiated islet cells and mesenchymal and/or adipose stem cells where the cells have been treated so as to facilitate redifferentiation. Further described herein are methods of generating Neo-Islets, the methods comprising: culturing a) dedifferentiated islet cells and mesenchymal and/or adipose stem cells; or b) redifferentiated islet cells and mesenchymal and/or adipose stem cells; on a surface that promotes the formation of cell clusters. Also described are methods of treating a subject, the methods comprising: providing to the subject Neo-Islets described herein. Additionally described are methods of treating a subject suffering from Type 1 Diabetes Mellitus, Type 2 Diabetes Mellitus, and other types of insulin-dependent diabetes mellitus, or impaired glucose tolerance by providing to the subject Neo-Islet as described herein. Additionally described are methods of treatment in which intraperitoneal administration of islet-sized Neo-Islets composed of high numbers of mesenchymal stem cells and cultured islet cells, durably and reversibly treats, without hypoglycemia, both streptozotocin-induced and spontaneous Type 1 Diabetes Mellitus, Type 2 Diabetes Mellitus, and other types of insulin-dependent diabetes mellitus, or impaired glucose tolerance.

    Abstract translation: 描述的是新胰岛，包括：a）去分化的胰岛细胞和间充质和/或脂肪干细胞; 或b）再分化的胰岛细胞和间充质和/或脂肪干细胞，其中细胞已被处理以促进再分化。 本文进一步描述的是产生新胰岛的方法，所述方法包括：培养a）去分化的胰岛细胞和间充质和/或脂肪干细胞; 或b）再分化的胰岛细胞和间充质和/或脂肪干细胞; 在促进细胞簇形成的表面上。 还描述了治疗受试者的方法，所述方法包括：向本文所述的受试者新胰岛提供。 另外描述的是通过向本文所述的受试者新胰岛提供治疗患有1型糖尿病，2型糖尿病和其他类型的胰岛素依赖性糖尿病的受试者或葡萄糖耐量降低的方法。 另外描述了治疗方法，其中腹膜内施用由大量间充质干细胞和培养的胰岛细胞组成的胰岛大小的新胰岛素，持续地和可逆地治疗，没有低血糖，链脲佐菌素诱导和自发的1型糖尿病 2糖尿病和其他类型的胰岛素依赖性糖尿病或葡萄糖耐量降低。

    Method for generating cancer stem cells from immortalized cell lines
    10.
    发明授权
    Method for generating cancer stem cells from immortalized cell lines 有权
    从永生化细胞系产生癌症干细胞的方法

    公开(公告)号：US09540614B2

    公开(公告)日：2017-01-10

    申请号：US14222697

    申请日：2014-03-24

    Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

    Inventor： Sunghoi Hong ,  Seung-Ick Oh ,  Hang-Soo Park

    IPC: C12N5/095

    CPC classification number: C12N5/0695 ,  C12N2502/1358 ,  C12N2506/25 ,  C12N2510/04

    Abstract: A method is described for generating a novel cancer stem cell line that possesses characteristics associated with stem cells, by co-culturing a human immortalized cell line and bone marrow-derived mesenchymal stem cells, and the novel cancer stem cell line established thereby. This method is able to readily generate cancer stem cells that are low in the level of structural chromosomal aberrations and are excellent in oncogenicity, and is effectively applicable to the development of anti-cancer drugs and personalized drugs.

    Abstract translation: 描述了通过共培养人类永生化细胞系和骨髓间充质干细胞以及由此建立的新型癌干细胞系来产生具有与干细胞相关特征的新型癌干细胞系的方法。 该方法能够容易地生成结构染色体畸变水平低的癌干细胞，致癌性优异，有效适用于抗癌药物和个性化药物的开发。

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