公开(公告)号：US20240052360A1

公开(公告)日：2024-02-15

申请号：US17642050

申请日：2021-08-19

申请人： KT&G CORPORATION

发明人： Hyo Seok SEO ,  Young Gi LEE ,  Jeong Heon LEE ,  Kwang Chul KIM ,  Woong Hyun NA ,  Kyoung Hwan OH ,  Eun Young JEON

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N9/22 ,  C12N15/11

CPC分类号： C12N15/8271 ,  C12N9/104 ,  C12Y203/02015 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20

摘要： Provided are: a plant cell, in which expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced as compared with a parent cell; a plant having that has reduced heavy metal absorption, and includes the plant cell; a method of reducing heavy metals in a plant, the method including reducing expression or activity of a PCS gene or a protein encoded by the PCS gene, as compared with a parent cell; a CRISPR-Cas9 recombinant vector including a single guide RNA targeting a PCS gene; and a method of preparing a plant with reduced heavy metals, the method including transforming a plant cell with the recombinant vector.

公开(公告)号：US20240229053A1

公开(公告)日：2024-07-11

申请号：US17928516

申请日：2022-05-31

申请人： KT&G CORPORATION

发明人： Hyo Seok SEO ,  Young Gi LEE ,  Jeong Heon LEE ,  Dong Sung JANG ,  Kwang Chul KIM ,  Woong Hyun NA

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N9/22

CPC分类号： C12N15/8243 ,  C12N9/1077 ,  C12N9/22 ,  C12N15/8205 ,  C12N2310/20 ,  C12Y204/02019

摘要： An aspect provides plant cells with an engineered QPT gene and methods of using the same. When a QPT gene in a plant cell is genetically engineered according to an aspect, biosynthesis of nicotine is effectively inhibited, and nicotine is reduced by about 97% or more, and since alkaloids other than nicotine (nornicotine, anabasine, anatabine) are also reduced by about 68% or more, plant cells with reduced carcinogen TSNAs (NNK, NNN, NAB, and NAT) may be produced.