公开(公告)号：US20100196958A1

公开(公告)日：2010-08-05

申请号：US12596399

申请日：2008-04-15

申请人： Ka-Yiu San ,  George N. Bennett ,  Thomas Bryce Causey ,  Matthew Sing Wong ,  Steven Wu

发明人： Ka-Yiu San ,  George N. Bennett ,  Thomas Bryce Causey ,  Matthew Sing Wong ,  Steven Wu

IPC分类号： C12P21/00 ,  C12N1/21

CPC分类号： C12P21/02

摘要： A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.

摘要翻译： 描述了使用具有灭活的ptsHI和野生型错误的细菌生产生物制品的方法，并且没有添加的葡萄糖转运活性并且消耗介质中几乎所有的葡萄糖。 ＆Dgr。ptsHI细菌产生大量的重组蛋白质，而不产生大量的乙酸盐。 细菌在标准LB肉汤上生长良好，无需额外补充。

公开(公告)号：US08236525B2

公开(公告)日：2012-08-07

申请号：US12596399

申请日：2008-04-15

申请人： Ka-Yiu San ,  George N. Bennett ,  Thomas Bryce Causey ,  Matthew Sing Wong ,  Steven Wu

发明人： Ka-Yiu San ,  George N. Bennett ,  Thomas Bryce Causey ,  Matthew Sing Wong ,  Steven Wu

IPC分类号： C12N1/20 ,  C12P21/06

CPC分类号： C12P21/02

摘要： A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.

摘要翻译： 描述了使用具有灭活的ptsHI和野生型错误的细菌生产生物制品的方法，并且没有添加的葡萄糖转运活性并且消耗介质中几乎所有的葡萄糖。 ＆Dgr。ptsHI细菌产生大量的重组蛋白质，而不产生大量的乙酸盐。 细菌在标准LB肉汤上生长良好，无需额外补充。