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公开(公告)号：US20100196958A1
公开(公告)日：2010-08-05
申请号：US12596399
申请日：2008-04-15
CPC分类号： C12P21/02
摘要： A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.
摘要翻译： 描述了使用具有灭活的ptsHI和野生型错误的细菌生产生物制品的方法，并且没有添加的葡萄糖转运活性并且消耗介质中几乎所有的葡萄糖。 ＆Dgr。ptsHI细菌产生大量的重组蛋白质，而不产生大量的乙酸盐。 细菌在标准LB肉汤上生长良好，无需额外补充。
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公开(公告)号：US08236525B2
公开(公告)日：2012-08-07
申请号：US12596399
申请日：2008-04-15
CPC分类号： C12P21/02
摘要： A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.
摘要翻译： 描述了使用具有灭活的ptsHI和野生型错误的细菌生产生物制品的方法，并且没有添加的葡萄糖转运活性并且消耗介质中几乎所有的葡萄糖。 ＆Dgr。ptsHI细菌产生大量的重组蛋白质，而不产生大量的乙酸盐。 细菌在标准LB肉汤上生长良好，无需额外补充。
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3.发明授权公开(公告)号：US07709261B2
公开(公告)日：2010-05-04
申请号：US11773408
申请日：2007-07-04
CPC分类号： C12N9/0036 , C12P1/00 , C12P7/625
摘要： The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.
摘要翻译： 本发明描述了一种用作生产还原化合物的方法的新型重组NADH再循环系统。 在具体实施方案中，还原的化合物包括乙醇，琥珀酸盐，乳酸盐，维生素，药物和生物降解的有机分子。 NADH回收系统在有氧和厌氧环境中影响还原途径的代谢通量。
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公开(公告)号：US07569380B2
公开(公告)日：2009-08-04
申请号：US11315453
申请日：2005-12-22
CPC分类号： C12N9/0006 , C12N9/1029 , C12P7/46 , C12P7/62
摘要： In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.
摘要翻译： 通过在使用乙酰辅酶A的途径中产生无效突变体和通过过表达使用NADH的产物并为了保持NADH和NAD +之间适当的氧化还原平衡，已经开发了从乙酰辅酶A生产酯的体内方法，例如乙酸异戊酯和琥珀酸酯。 。 该方法以ldhA，adhE，ackA-pta中的无效突变和丙酮酸羧化酶和醇乙酰转移酶的过表达为例。 该菌株产生较高水平的乙酸异戊酯和琥珀酸酯。
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公开(公告)号：US20130203137A1
公开(公告)日：2013-08-08
申请号：US13696268
申请日：2011-05-03
申请人： Ka-Yiu San , George N. Bennett , Grant J. Balzer , Jiangfeng Zhu , Chandresh Thakker , Ailen M. Sanchez
发明人： Ka-Yiu San , George N. Bennett , Grant J. Balzer , Jiangfeng Zhu , Chandresh Thakker , Ailen M. Sanchez
CPC分类号： C12P7/46 , C12N9/0008 , C12N9/93 , C12N15/52 , C12N15/70 , C12Y102/01002 , C12Y604/01001
摘要： Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and overexpress In one embodiment, the bacteria are ΔadhEΔldhAΔiclRΔack-pta plus PYC+ and NAD+-dependant FDH+.
摘要翻译： 用于制备琥珀酸盐和来自克雷伯斯循环的其它4碳二羧酸盐的改进的细菌具有改变以减少乙酸盐，乳酸盐，乙醇和甲酸盐，以及开启乙醛酸盐分流，产生更多的NADH和过表达。在一个实施方案中，细菌为DeltaadhEDeltaldhADeltaiclRDeltaack-pta 加上PYC +和NAD +依赖性FDH +。
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6.发明授权High molar succinate yield bacteria by increasing the intracellular NADH availability 有权高摩尔琥珀酸盐通过增加细胞内NADH的可用性而产生细菌公开(公告)号：US07927859B2
公开(公告)日：2011-04-19
申请号：US10923635
申请日：2004-08-20
申请人： Ka-Yiu San , George N. Bennett , Ailen Sánchez
发明人： Ka-Yiu San , George N. Bennett , Ailen Sánchez
IPC分类号： C12N1/20 , C12N9/02 , C12N9/04 , C12N9/10 , C12N15/00 , C12P7/64 , C12P21/04 , C12Q1/68 , C12Q1/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/46
摘要： The invention relates to increasing the yield of succinate in bacteria by increasing the intracellular availability of cofactors such as NADH.
摘要翻译： 本发明涉及通过增加辅因子如NADH的细胞内可用性来提高细菌中琥珀酸的产量。
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公开(公告)号：US07790416B2
公开(公告)日：2010-09-07
申请号：US11697295
申请日：2007-04-05
申请人： Ka-Yiu San , George N. Bennett , Ailen Sanchez
发明人： Ka-Yiu San , George N. Bennett , Ailen Sanchez
摘要： The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.
摘要翻译： 本发明涉及一种细菌的突变菌株，其缺乏或含有若干关键代谢酶的突变基因，并且在厌氧条件下产生大量琥珀酸。
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8.发明申请公开(公告)号：US20100184195A1
公开(公告)日：2010-07-22
申请号：US12705170
申请日：2010-02-12
IPC分类号： C12N1/21
CPC分类号： C12N9/0036 , C12P1/00 , C12P7/625
摘要： The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.
摘要翻译： 本发明描述了一种用作生产还原化合物的方法的新型重组NADH再循环系统。 在具体实施方案中，还原的化合物包括乙醇，琥珀酸盐，乳酸盐，维生素，药物和生物降解的有机分子。 NADH回收系统在有氧和厌氧环境中影响还原途径的代谢通量。
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公开(公告)号：US07326557B2
公开(公告)日：2008-02-05
申请号：US10987265
申请日：2004-11-12
申请人： Ka-Yiu San , George N. Bennett , Ailen Sanchez
发明人： Ka-Yiu San , George N. Bennett , Ailen Sanchez
IPC分类号： C12N1/12 , C12N15/63 , C12Q1/68 , G01N33/554 , C07H21/02
CPC分类号： C12P19/36
摘要： A method of increasing cellular NADPH levels by expressing one or more genes that encode a enzyme that causes the production of NADPH. The system can be combined with other enzymes that require NADPH, thus improving the overall production of the desired protein.
摘要翻译： 通过表达编码引起NADPH产生的酶的一种或多种基因来增加细胞NADPH水平的方法。 该系统可以与需要NADPH的其它酶组合，从而提高所需蛋白质的总体产量。
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公开(公告)号：US07244610B2
公开(公告)日：2007-07-17
申请号：US10987511
申请日：2004-11-12
申请人： Ka-Yiu San , George N. Bennett , Henry Lin
发明人： Ka-Yiu San , George N. Bennett , Henry Lin
摘要： Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
摘要翻译： 提供了使用需氧培养方法和多突变大肠杆菌菌株提高琥珀酸产量的方法。 还提供了产生大量琥珀酸的大肠杆菌的突变菌株。
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