公开(公告)号：US20090215079A1

公开(公告)日：2009-08-27

申请号：US12096073

申请日：2006-12-01

申请人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Michael Mertig ,  Justin Gooding ,  Gerhard Rödel ,  Karolina Ihle

发明人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Michael Mertig ,  Justin Gooding ,  Gerhard Rödel ,  Karolina Ihle

IPC分类号： G01N33/53 ,  C12M1/00 ,  G01N27/26

CPC分类号： G01N33/56911 ,  G01N2333/245 ,  G01N2333/25 ,  G01N2333/255 ,  G01N2333/28

摘要： In a method for detection of microorganisms and/or their activity with biosensors, on a surface of a substrate over portions thereof at least one ligand for binding a receptor or at least one receptor for a ligand or at least one ligand for binding a receptor and at least one receptor for either the one or a further ligand are immobilized chemically, physically, or biologically, wherein physical or physicochemical changes, caused at receptors by binding of ligands that are emitted by microorganisms in the process of quorum sensing are measured.

摘要翻译： 在用于用生物传感器检测微生物和/或其活性的方法中，在基底表面上的至少一个用于结合受体的配体或用于配体的至少一个受体或至少一个用于结合受体的配体的配体 测量一个或另一个配体的至少一个受体化学，物理或生物学上的固定，其中测量在受感染过程中通过结合由微生物发出的配体在受体处引起的物理或物理化学变化。

公开(公告)号：US20100285505A1

公开(公告)日：2010-11-11

申请号：US12666299

申请日：2008-06-27

申请人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Mathias Lakatos ,  Michael Mertig ,  Gerhard Rödel ,  Simone Thierfelder

发明人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Mathias Lakatos ,  Michael Mertig ,  Gerhard Rödel ,  Simone Thierfelder

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/587 ,  G01N33/54373 ,  G01N2333/395

摘要： The invention relates to devices and methods for detecting a substance by cell surface polarizations and the detection thereof by means of PPR or particle-mediated fluorescence. The device for detecting a substance by cell surface polarization according to the invention has cells, wherein a gene the expression of which leads to the polarized presentation of a protein on the surface of the cell is placed under the control of a promotor which can be regulated by the substance to be detected, nanoparticles which are functionalized with a molecule which can bind specifically to the surface-exposed protein, and at least one optical measurement device, such that an accumulation of the nanoparticles on the surface of the cells can be detected by particle plasmon resonance or particle-mediated fluorescence.

摘要翻译： 本发明涉及用于通过细胞表面极化检测物质的装置和方法，并且其通过PPR或颗粒介导的荧光进行检测。 根据本发明的用于通过细胞表面极化检测物质的装置具有细胞，其中表达导致细胞表面上的蛋白质的极化表达的基因置于可调节的启动子的控制下 通过待检测的物质，用能够与表面暴露蛋白特异性结合的分子官能化的纳米颗粒和至少一种光学测量装置，使得纳米颗粒在细胞表面上的积聚可以通过 颗粒等离子体共振或粒子介导的荧光。

公开(公告)号：US20090124488A1

公开(公告)日：2009-05-14

申请号：US11997163

申请日：2006-07-29

申请人： Jurgen Hofinger ,  Wolfgang Pompe ,  Michael Mertig ,  Alexander Kirchner ,  Nina Schreiber ,  Anja Bluher ,  Steffen Roos ,  Daniela Keck ,  Beate Katzschner

发明人： Jurgen Hofinger ,  Wolfgang Pompe ,  Michael Mertig ,  Alexander Kirchner ,  Nina Schreiber ,  Anja Bluher ,  Steffen Roos ,  Daniela Keck ,  Beate Katzschner

IPC分类号： B05D3/10 ,  B32B15/00 ,  B01J31/02 ,  B03C3/40

CPC分类号： C23C18/1889 ,  C23C18/31 ,  Y10T428/31678 ,  Y10T428/31681

摘要： A substrate with spatially selective metal coating is produced by first applying biological templates onto parts of the surface of the substrate and applying a metal coating only once the biological templates have been deposited on the substrate. The biological templates are for example surface layer proteins (S-layer) and the metal coating is a noble metal coating. The substrates with spatially selective metal coating are used in catalysts or solid-state electrolyte sensors.

摘要翻译： 具有空间选择性金属涂层的基底是通过首先将生物模板施加到基底表面的部分上并且仅在生物模板沉积在基底上之后施加金属涂层来制备的。 生物模板是例如表层蛋白（S层），金属涂层是贵金属涂层。 具有空间选择性金属涂层的基板用于催化剂或固态电解质传感器。

公开(公告)号：US20090173527A1

公开(公告)日：2009-07-09

申请号：US11990265

申请日：2006-07-27

申请人： Annegret Benke ,  Gerald Eckstein ,  Oliver Jost ,  Michael Mertig ,  Daniel Sickert ,  Sebastian Taeger

发明人： Annegret Benke ,  Gerald Eckstein ,  Oliver Jost ,  Michael Mertig ,  Daniel Sickert ,  Sebastian Taeger

IPC分类号： H05K1/11 ,  C25B7/00

CPC分类号： H01L51/102 ,  B82Y10/00 ,  B82Y30/00 ,  H01L51/0048

摘要： A nanostructure is provided on a substrate by forming at least one multi-electrode arrangement on the substrate, wherein said electrodes comprise respective electrode areas projected with respect to the opposite electrode ends which extend along a line in such a way that the adjacent ends produce a respectively frequency time-variable potential difference. A suspension of nano-object such as nanotubes, nanowires and/or carbon nanotubes is produced and then transferred to the substrate between the adjacent ends. The assembly of respective individual nano-objects is dielectrophoreticly deposited on the line between said adjacent ends, and the assembly of respective nano-objects is fused in the area of the ends in such a way that the nanostructure is formed.

摘要翻译： 通过在衬底上形成至少一个多电极布置在衬底上提供纳米结构，其中所述电极包括相对于相对电极端部突出的相应电极区域，所述相对电极端部沿着一条线延伸，使得相邻端部产生 分别为频率时变电位差。 产生纳米材料如纳米管，纳米线和/或碳纳米管的悬浮液，然后转移到相邻端之间的基底。 各个单独的纳米物体的组件被介电电泳沉积在所述相邻端之间的线上，并且各个纳米物体的组件以端部的区域融合，使得形成纳米结构。