Generation of high polyhydroxybutyrate producing oilseeds
    3.
    发明授权
    Generation of high polyhydroxybutyrate producing oilseeds 有权
    生产高聚羟基丁酸酯油籽油

    公开(公告)号:US09181559B2

    公开(公告)日:2015-11-10

    申请号:US13395702

    申请日:2010-09-15

    CPC分类号: C12N15/8214 C12N15/8257

    摘要: Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHA”) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHB”). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

    摘要翻译: 提供了用于合成生物聚合物的转基因植物,植物材料,植物细胞和遗传构建体,例如聚羟基链烷酸酯(“PHA”)。 在一个实施方案中,转基因植物合成聚羟基丁酸酯(“PHB”)。 在一个实施方案中,转基因植物编码编码用于产生PHA的酶的一种或多种基因的siRNA。 在更优选的实施方案中,siRNA表达受诱导型调节元件的控制。 在另一个实施方案中,转基因植物含有编码将降解聚合物的表达酶的转基因。 在优选的实施方案中,这些酶的表达受发芽特异性,诱导型或最小启动子的控制。 在另一个实施方案中,转基因植物含有编码增加聚合物合成碳流的酶的转基因。 在优选的实施方案中,这些转基因编码增加卡尔文循环中碳流的酶。

    METHOD OF POSITIVE PLANT SELECTION USING SORBITOL DEHYDROGENASE
    5.
    发明申请
    METHOD OF POSITIVE PLANT SELECTION USING SORBITOL DEHYDROGENASE 审中-公开
    使用SORBITOL DEHYDROGENASE的积极植物选择方法

    公开(公告)号:US20110321190A1

    公开(公告)日:2011-12-29

    申请号:US13223575

    申请日:2011-09-01

    CPC分类号: C12N15/821 C12N9/0006

    摘要: Transgenic plants and methods of culturing them using sorbitol as a sole carbon source are provided. One embodiment provides a method and system for positively selecting transgenic plants carrying and expressing a gene of interest. The transgenic plants are engineered to express sorbitol dehydrogenase in an amount effective to allow the transgenic plant to grow using sorbitol as the sole carbon source. In a preferred embodiment, the plant to be transformed does not have endogenous sorbitol dehydrogenase activity. Representative plants that can be transformed, include but are not limited to members of the Brassica family, industrial oilseeds, Arabidopsis thaliana, algae, soybean, cottonseed, sunflower, palm, coconut, rice, safflower, peanut, mustards, silage corn, alfalfa, switchgrass, miscanthus, sorghum, tobacco, sugarcane and flax.

    摘要翻译: 提供了转基因植物和使用山梨醇作为唯一碳源培养它们的方法。 一个实施方案提供了用于正选择携带并表达感兴趣的基因的转基因植物的方法和系统。 转基因植物被工程化以表达山梨糖醇脱氢酶,其数量有效地允许转基因植物使用山梨糖醇作为唯一碳源来生长。 在优选的实施方案中,待转化的植物不具有内源山梨醇脱氢酶活性。 可以转化的代表性植物包括但不限于芸苔属家族,工业油籽,拟南芥,藻类,大豆,棉籽,向日葵,棕榈,椰子,大米,红花,花生,芥菜,青贮玉米,苜蓿, 柳枝稷,芒草,高粱,烟草,甘蔗和亚麻。