公开(公告)号：US3937816A

公开(公告)日：1976-02-10

申请号：US447939

申请日：1974-03-04

申请人： Karl-Heinz Jaeger ,  Hellmut Mittenzwei

发明人： Karl-Heinz Jaeger ,  Hellmut Mittenzwei

IPC分类号： A61K35/28

CPC分类号： A61K35/28

摘要： Compositions derived from spleens of vertebrata and process for obtaining the said compositions which are useful for increasing or decreasing the normal rate of tissue growth.

公开(公告)号：US3973001A

公开(公告)日：1976-08-03

申请号：US542910

申请日：1975-01-22

申请人： Karl-Heinz Jaeger ,  Hellmut Mittenzwei

发明人： Karl-Heinz Jaeger ,  Hellmut Mittenzwei

IPC分类号： A61K35/14 ,  A61K37/00

CPC分类号： A61K35/14 ,  Y10S530/829

摘要： Novel blood extracts of vertebrata, i.e. animals having a vertebral column, especially warm blooded animals, which blood extracts possess cell respiration stimulating activity and stimulate the growth of cells and to novel processes for the preparation of the said blood extracts.

摘要翻译： 脊椎动物的新鲜血液提取物，即具有脊柱的动物，特别是温血动物，其中血液提取物具有细胞呼吸刺激活性并刺激细胞的生长以及用于制备所述血液提取物的新方法。

公开(公告)号：US4116948A

公开(公告)日：1978-09-26

申请号：US838486

申请日：1977-10-03

申请人： Hellmut Mittenzwei ,  Janez Terpin

发明人： Hellmut Mittenzwei ,  Janez Terpin

IPC分类号： A61K38/17 ,  A61K38/28 ,  C07G7/00

CPC分类号： A61K38/1709 ,  A61K38/28

摘要： The invention concerns a process for removing inorganic salts from a liquid medium having a solids content of from about 25 to about 65 mg/ml and containing active organic peptide substances of a molecular weight of from about 500 to about 10,000 and up to 70% by weight inorganic salts based on the solids content of the liquid medium, which comprises the steps of transferring said liquid medium onto a filtration gel layer carried on a perforated centrifuge drum at a liquid medium: gel volume ratio of from about 1:2.5 to about 1:4.5, said filtration gel layer being higher cross-linked and having a particle size of from about 40 to 120 .mu., centrifuging at a centrifugal force of from about 800 to about 1500 g, and collecting the liquid centrifugate containing the active organic peptide substances and a low amount of salt.The substance recovered by the process possesses growth regulating properties and can be recovered from mammalian blood or serum.

公开(公告)号：US4085204A

公开(公告)日：1978-04-18

申请号：US748956

申请日：1976-12-09

申请人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

发明人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

IPC分类号： A61K35/14 ,  A61K38/00 ,  A61K38/16 ,  A61K38/28 ,  A61P3/08 ,  A61P3/10 ,  A61P5/00 ,  C07K4/12 ,  C07K14/435 ,  C07K14/625 ,  A61K35/16 ,  A61K37/02

CPC分类号： C07K4/12 ,  C07K14/625 ,  Y10S530/829 ,  Y10S530/83

摘要： The invention concerns a novel process for obtaining insulin-like active substances from blood or a blood component of a warm-blooded animal, which comprises the steps of pre-treating the blood or blood component by adjusting the pH thereof to a value of from 2 to 4 or of from 8 to 10, incubating the pre-treated blood at a temperature of from about 15.degree. to about 40.degree. C for a period of at least four hours, cooling the incubated blood to a temperature of less than 10.degree. C, and recovering from the treated blood after neutralization organic peptide substances having a molecular weight of from about 1000 to about 5000, and an insulin-like activity of from about 14 to about 40 .mu.U/mg solid.

摘要翻译： 本发明涉及一种从温血动物血液或血液成分中获得胰岛素样活性物质的新方法，其包括以下步骤：通过将其pH值调节至2以预处理血液或血液成分 至4或8至10℃，将预处理血液在约15℃至约40℃的温度下孵育至少4小时，将培养的血液冷却至低于10℃的温度 并且在中和后的处理血液中回收具有约1000至约5000的分子量的有机肽物质和约14至约40μU/ mg固体的胰岛素样活性。

公开(公告)号：US4139611A

公开(公告)日：1979-02-13

申请号：US848109

申请日：1977-11-03

申请人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

发明人： Adolf Wacker ,  Dorthe Wacker ,  Klaus Pohler ,  Hellmut Mittenzwei

IPC分类号： C07K4/12 ,  C07K14/625 ,  A61K35/14 ,  A61K37/00 ,  A61K37/26

CPC分类号： C07K14/625 ,  C07K4/12 ,  Y10S530/829 ,  Y10S530/83

摘要： Organic peptide containing substances with insulin-like activity derived from blood or a blood component of a warm-blooded animal, which comprises the steps of pre-treating the blood or blood component by adjusting the pH thereof to a value of from 2 to 4 or of from 8 to 10, incubating the pre-treated blood at a temperature of from about 15 to about 40.degree. C for a period of at least four hours, cooling the incubated blood to a temperature of less than 10.degree. C, and recovering from the treated blood after neutralization organic peptide substances having a molecular weight of from about 1000 to about 5000, and an insulin-like activity of from about 14 to about 40 .mu.U/mg solid.

摘要翻译： 含有源自血液或温血动物血液成分的胰岛素样活性的含有有机肽的物质包括以下步骤：通过将其pH值调节至2至4或 8至10℃，将预处理血液在约15至约40℃的温度下孵育至少4小时，将孵育的血液冷却至低于10℃的温度，并从 处理后的血液中和有机肽物质的分子量为约1000至约5000，胰岛素样活性为约14至约40微米U / mg固体。