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    Trichoderma reesei G/11 xylanases with improved stability
    3.
    发明授权
    Trichoderma reesei G/11 xylanases with improved stability 有权
    具有改善稳定性的里氏木霉G / 11木聚糖酶

    公开(公告)号：US07718411B1

    公开(公告)日：2010-05-18

    申请号：US10912272

    申请日：2004-08-05

    申请人： Fred Fenel ,  Ossi Turunen ,  Matti Leisola

    发明人： Fred Fenel ,  Ossi Turunen ,  Matti Leisola

    IPC分类号： C12N9/24 ,  C12N9/42 ,  C07H21/04

    CPC分类号： C12N9/2482 ,  C12Y302/01008

    摘要： The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.

    摘要翻译： 本发明涉及蛋白质工程，尤其涉及家族G / 11木聚糖酶，以及编码所述酶的基因。 具体而言，本发明涉及里氏木霉XYNII基因，其编码内切-1,4-纤维素 - 木聚糖酶（EC 3.2.1.8）。 本发明描述了如何使用定点诱变来改进酶的性质以匹配其使用的工业条件。 蛋白质工程可用于提高木聚糖酶的热活性和热稳定性，并扩大其pH范围。

    Method to improve the stability and broaden the pH range of family G/11 xylanases
    4.
    发明授权
    Method to improve the stability and broaden the pH range of family G/11 xylanases 有权
    提高G / 11木聚糖酶稳定性和扩大pH范围的方法

    公开(公告)号：US08426181B2

    公开(公告)日：2013-04-23

    申请号：US11963285

    申请日：2007-12-21

    申请人： Fred Fenel ,  Ossi Turunen ,  Matti Leisola

    发明人： Fred Fenel ,  Ossi Turunen ,  Matti Leisola

    IPC分类号： C12N9/24 ,  C12N9/42 ,  C07H21/04 ,  A23D9/013 ,  D23C3/20 ,  C09B67/00

    CPC分类号： C12N9/2402 ,  C12N9/2482 ,  C12Y302/01008

    摘要： The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.

    摘要翻译： 本发明涉及蛋白质工程，尤其涉及家族G / 11木聚糖酶，以及编码所述酶的基因。 具体而言，本发明涉及编码内切-1,4-β-木聚糖酶（EC 3.2.1.8）的里氏木霉XYNII基因。 本发明描述了如何使用定点诱变来改进酶的性质以匹配其使用的工业条件。 蛋白质工程可用于提高木聚糖酶的热活性和热稳定性，并扩大其pH范围。

    Method to Improve the Stability and Broaden the pH range of family G/11 xylanases
    7.
    发明申请
    Method to Improve the Stability and Broaden the pH range of family G/11 xylanases 有权
    提高稳定性和扩大家族G / 11木聚糖酶pH范围的方法

    公开(公告)号：US20080171374A1

    公开(公告)日：2008-07-17

    申请号：US11963285

    申请日：2007-12-21

    申请人： Fred Fenel ,  Ossi Turunen ,  Matti Leisola

    发明人： Fred Fenel ,  Ossi Turunen ,  Matti Leisola

    IPC分类号： C12N9/42 ,  C12N9/00 ,  D21C3/20 ,  C09B67/00 ,  A23K1/165

    CPC分类号： C12N9/2402 ,  C12N9/2482 ,  C12Y302/01008

    摘要： The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.

    摘要翻译： 本发明涉及蛋白质工程，尤其涉及家族G / 11木聚糖酶，以及编码所述酶的基因。 具体而言，本发明涉及编码内切-1,4-β-木聚糖酶（EC 3.2.1.8）的里氏木霉XYNII基因。 本发明描述了如何使用定点诱变来改进酶的性质以匹配其使用的工业条件。 蛋白质工程可用于提高木聚糖酶的热活性和热稳定性，并扩大其pH范围。