公开(公告)号：US06607662B1

公开(公告)日：2003-08-19

申请号：US09655070

申请日：2000-09-05

申请人： Katsunori Ikeda ,  Yoshiaki Nishiya ,  Masahiro Kusumoto ,  Masahiro Suzuki ,  Yoshihisa Kawamura

发明人： Katsunori Ikeda ,  Yoshiaki Nishiya ,  Masahiro Kusumoto ,  Masahiro Suzuki ,  Yoshihisa Kawamura

IPC分类号： B01L302

CPC分类号： G01N35/0098 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N1/34 ,  G01N35/028 ,  C12Q2563/143

摘要： The present invention provides an apparatus for purifying nucleic acids such as DNA and RNA or proteins such as enzymes and antibodies from microorganisms such as viruses and bacteria or animal and plant tissues, the apparatus being capable of fast processing of a plurality of samples. More specifically, the present invention provides an apparatus for purifying nucleic acids or proteins using magnetically attractable particles, the apparatus comprising a plurality of piston pumps; a plurality of nozzles capable of having a plurality of disposable tips which are automatically attachable/detachable; and a mechanism which is capable of dispensing a desired amount of a reagent to be used subsequently in the next step into the same number of sections as that of the samples with high accuracy and at a high rate, while a mixture of a sample and a reagent are being mixed (stirred) by the pumps and nozzles, the apparatus being capable of rapidly processing a plurality of samples, wherein a series of steps from mixing (stirring) of the magnetically attractable particles and concentration of the sample to purification is automated with reduced wastes.

公开(公告)号：US06986848B2

公开(公告)日：2006-01-17

申请号：US10235496

申请日：2002-09-06

申请人： Katsunori Ikeda ,  Yoshiaki Nishiya ,  Masahiro Kusumoto ,  Masahiro Suzuki ,  Yoshihisa Kawamura

发明人： Katsunori Ikeda ,  Yoshiaki Nishiya ,  Masahiro Kusumoto ,  Masahiro Suzuki ,  Yoshihisa Kawamura

IPC分类号： B03C1/01 ,  B01D35/06 ,  B01L3/02

CPC分类号： G01N35/0098 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N1/34 ,  G01N35/028 ,  C12Q2563/143

摘要： A method for purifying nucleic acids or proteins comprising a plurality of piston pumps; and a plurality of nozzles capable of having a plurality of disposable tips which are automatically attachable/detachable. The apparatus includes a mechanism for mixing a liquid mixture of each sample and a reagent in a plurality of sections of a container by sucking up a plurality of the mixtures into tips, followed by discharging the mixtures in the sections simultaneously. The apparatus also includes a mechanism for dispensing a desired amount of a reagent to be used subsequently into the same number of sections of a different container as samples, while the mixing is in progress.

摘要翻译： 一种纯化包含多个活塞泵的核酸或蛋白质的方法; 以及能够具有可自动附接/拆卸的多个一次性尖端的多个喷嘴。 该装置包括一种机构，用于通过将多个混合物吸入到尖端中，然后将该混合物同时排出，将每个样品的液体混合物和试剂混合在容器的多个部分中。 该设备还包括一种用于在混合进行期间分配期望量的试剂以随后用于与样品相同数量的不同容器的部分的机构。

公开(公告)号：US07381467B2

公开(公告)日：2008-06-03

申请号：US11081537

申请日：2005-03-17

申请人： Satoko Arai ,  Mikio Kishimoto ,  Kenji Kohno ,  Masahiro Kusumoto ,  Yoshiaki Nishiya

发明人： Satoko Arai ,  Mikio Kishimoto ,  Kenji Kohno ,  Masahiro Kusumoto ,  Yoshiaki Nishiya

IPC分类号： B32B5/16

CPC分类号： C09K11/7706 ,  C01G49/009 ,  C01P2004/03 ,  C01P2004/62 ,  C01P2006/42 ,  C01P2006/60 ,  C09K11/06 ,  G01N33/5434 ,  G01N33/582 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2993

摘要： The present invention provides composite particles which have magnetism and simultaneously emit fluorescence with a variety of wavelengths, and which are suitable for use in the fields of biology, biochemistry or the like. The composite particles of the present invention comprise ferromagnetic iron oxide particles, fluorescent pigment particles and silica, and have an average particle size of 1 to 10 μm, a coercive force of 2.39 to 11.94 kA/m (30 to 150 oersted), saturation magnetization of 0.5 to 40 A.m2/kg (0.5 to 40 emu/g). The peak value of the wavelength of fluorescence from the composite particle is in the range of 350 to 750 nm, when the composite particle is excited by light with a wavelength of 250 to 600 nm.

摘要翻译： 本发明提供具有磁性并且同时发出具有各种波长的荧光并且适用于生物学，生物化学等领域的复合颗粒。 本发明的复合颗粒包含铁磁性氧化铁颗粒，荧光颜料颗粒和二氧化硅，平均粒径为1-10μm，矫顽力为2.39至11.94kA / m（30至150奥斯特），饱和磁化强度 为0.5〜40Am 2 / kg（0.5〜40emu / g）。 当复合颗粒被波长为250至600nm的光激发时，来自复合颗粒的荧光波长的峰值在350至750nm的范围内。

公开(公告)号：US20060186055A1

公开(公告)日：2006-08-24

申请号：US10564563

申请日：2004-07-13

申请人： Masahiro Kusumoto ,  Yoshiaki Nishiya ,  Mikio Kishimoto ,  Nobuhiro Umebayashi

发明人： Masahiro Kusumoto ,  Yoshiaki Nishiya ,  Mikio Kishimoto ,  Nobuhiro Umebayashi

IPC分类号： C02F1/48

CPC分类号： B03C1/01

摘要： The present invention provides a device relating to separation (extraction, purification) of a biological component such as nucleic acid, protein and the like from a liquid sample containing the biological component, and, as a method, a device for separating a biological component, which contains magnetically responsive particles and a chip obtained by adhering a pair of substrates, which contains one or multiple grooves formed on at least one surface thereof, with the groove(s) placed inside, and a method of separating a biological component from a liquid sample, which uses this device, and includes the following steps (a)-(d): (a) a step of holding the device such that the adhesion surface of the pair of substrates is about perpendicular to the horizontal direction, (b) a step of adsorbing the biological component to magnetically responsive particles by contacting the magnetically responsive particles with the liquid sample containing the biological component, (c) a step of separating the magnetically responsive particles containing the biological component adsorbed thereto from the liquid sample, and (d) a step of separating the biological component from the magnetically responsive particles.

摘要翻译： 本发明提供了从含有生物成分的液体样品中分离（提取，纯化）生物成分如核酸，蛋白质等的装置，作为分离生物成分的装置， 其包含磁响应性颗粒和通过将包含形成在其至少一个表面上的一个或多个凹槽的一对基板与设置在内部的凹槽粘附而获得的芯片，以及将生物组分与液体分离的方法 （a） - （d）：（a）保持所述装置的步骤，使得所述一对基板的粘附表面垂直于水平方向，（b）使用该装置的样品，并且包括以下步骤 通过使磁响应性颗粒与含有生物成分的液体样品接触，将生物成分吸附到磁响应性颗粒上的步骤，（c）分离步骤 g含有从液体样品吸附到其上的生物成分的磁响应颗粒，和（d）将生物成分与磁响应颗粒分离的步骤。

公开(公告)号：US20060062999A1

公开(公告)日：2006-03-23

申请号：US11081537

申请日：2005-03-17

申请人： Satoko Arai ,  Mikio Kishimoto ,  Kenji Kohno ,  Masahiro Kusumoto ,  Yoshiaki Nishiya

发明人： Satoko Arai ,  Mikio Kishimoto ,  Kenji Kohno ,  Masahiro Kusumoto ,  Yoshiaki Nishiya

IPC分类号： B32B5/16

CPC分类号： C09K11/7706 ,  C01G49/009 ,  C01P2004/03 ,  C01P2004/62 ,  C01P2006/42 ,  C01P2006/60 ,  C09K11/06 ,  G01N33/5434 ,  G01N33/582 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2993

摘要： The present invention provides composite particles which have magnetism and simultaneously emit fluorescence with a variety of wavelengths, and which are suitable for use in the fields of biology, biochemistry or the like. The composite particles of the present invention comprise ferromagnetic iron oxide particles, fluorescent pigment particles and silica, and have an average particle size of 1 to 10 μm, a coercive force of 2.39 to 11.94 kA/m (30 to 150 oersted), saturation magnetization of 0.5 to 40 A.m2/kg (0.5 to 40 emu/g). The peak value of the wavelength of fluorescence from the composite particle is in the range of 350 to 750 nm, when the composite particle is excited by light with a wavelength of 250 to 600 nm.

摘要翻译： 本发明提供具有磁性并且同时发出具有各种波长的荧光并且适用于生物学，生物化学等领域的复合颗粒。 本发明的复合颗粒包含铁磁性氧化铁颗粒，荧光颜料颗粒和二氧化硅，平均粒径为1-10μm，矫顽力为2.39至11.94kA / m（30至150奥斯特），饱和磁化强度 为0.5〜40Am 2 / kg（0.5〜40emu / g）。 当复合颗粒被波长为250至600nm的光激发时，来自复合颗粒的荧光波长的峰值在350至750nm的范围内。