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    Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium
    2.
    发明授权
    Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium 失效
    用于体外培养猪胚胎的培养基和使用培养基体外生产猪胚胎的方法

    公开(公告)号：US07060496B2

    公开(公告)日：2006-06-13

    申请号：US10790113

    申请日：2004-03-02

    申请人： Kazuhiro Kikuchi ,  Hiroyuki Kaneko ,  Junko Noguchi

    发明人： Kazuhiro Kikuchi ,  Hiroyuki Kaneko ,  Junko Noguchi

    IPC分类号： C12N5/06 ,  C12N5/00

    CPC分类号： C12N5/0604 ,  C12N2500/30 ,  C12N2502/243

    摘要： The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along with a method for in vitro culturing in vitro-produced porcine embryo using the culture medium, which can improve the quality of the resulting blastocyst and can develop the blastocyst into fetus and infant after transfer.A culture medium for the in vitro culture of in vitro-produced porcine embryo, which contains lactic acid and pyruvic acid; a culture medium for the in vitro culture, which is conditioned with oviductal epithelial cell; and a method for in vitro culturing in vitro-produced porcine embryo, comprising culturing the in vitro-produced porcine embryo using the culture medium for the 0 to 2-day term after fertilization and subsequently culturing the embryo in a glucose-containing culture medium.

    摘要翻译： 本发明提供了用于体外培养的体外培养的猪胚胎的体外培养基，其可以改善所产生的囊胚的质量，并且可以提高转移后胎儿和婴儿的发育比例以及方法 用于体外培养体外产生的使用培养基的猪胚胎，其可以改善所产生的囊胚的质量，并且可以在转移后将囊胚发育成胎儿和婴儿。 用于体外培养体外培养的含有乳酸和丙酮酸的猪胚胎的培养基; 用于体外培养的培养基，其用输卵管上皮细胞调节; 以及体外培养体外产生的猪胚胎的方法，包括使用培养基培养体外产生的猪胚胎，在受精后0至2天，随后在含葡萄糖的培养基中培养胚胎。