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    CELL CULTURE MEDIA COMPOSITION AND METHODS OF PRODUCING THEREOF
    3.
    发明申请
    CELL CULTURE MEDIA COMPOSITION AND METHODS OF PRODUCING THEREOF 审中-公开
    细胞培养基组合物及其生产方法

    公开(公告)号：US20160017277A1

    公开(公告)日：2016-01-21

    申请号：US14773299

    申请日：2014-02-17

    申请人： NEUCLONE BIOLOGICS PTY LTD

    发明人： Noelle Sunstrom

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0037 ,  C12N5/0056 ,  C12N5/0682 ,  C12N2500/90 ,  C12N2501/305 ,  C12N2502/243 ,  C12N2502/99

    摘要： A serum free cell culture media, wherein the media is adapted to be conditioned by culturing a first set of eukaryotic cells in the media, wherein the first set of eukaryotic cells use an expression vector to excrete levels of desired complex proteins into the media; wherein said desired complex proteins include human Growth Hormone (hGH), Growth Hormone-like growth factors, insulin-like growth factors, insulin, modified insulins, cytokines, mitogenic proteases and mixtures thereof; and wherein the media is adapted to grow a set of eukaryotic cells.

    摘要翻译： 一种无血清细胞培养基，其中所述培养基适于通过在所述培养基中培养第一组真核细胞进行调节，其中所述第一组真核细胞使用表达载体将所需复合蛋白的水平排出到培养基中; 其中所述所需的复合蛋白包括人生长激素（hGH），生长激素样生长因子，胰岛素样生长因子，胰岛素，修饰的胰岛素，细胞因子，促有丝分裂蛋白酶及其混合物; 并且其中所述培养基适于生长一组真核细胞。

    Methods for co-culturing cord blood cells or cord tissue with menstrual multipotent cells
    4.
    发明申请
    Methods for co-culturing cord blood cells or cord tissue with menstrual multipotent cells 审中-公开
    与月经多能细胞共培养脐带血细胞或脐带组织的方法

    公开(公告)号：US20120208275A1

    公开(公告)日：2012-08-16

    申请号：US13209123

    申请日：2011-08-12

    申请人： Mercedes A. Walton ,  Julie G. Allickson

    发明人： Mercedes A. Walton ,  Julie G. Allickson

    IPC分类号： C12N5/078

    CPC分类号： C12N5/0647 ,  C12N2502/137 ,  C12N2502/1388 ,  C12N2502/243

    摘要： Methods are provided for obtaining expanded human cord blood cells or cord tissue cells expressing CD34. The methods involve seeding a sufficient amount of human cord blood cells or cord tissue cells with a sufficient amount of menstrual cells under co-culture conditions suitable to promote expansion of the cord cells, and co-culturing the cord cells with the menstrual cells under culture conditions that support at least two or more population doublings of the cord cells. Methods are also provided for growing expanded human cord cells to give rise to any one of colony forming units, colony forming unit granulocyte macrophages (CFU-GM), burst forming unit erythroids (BFU-E), and colony forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU-GEMM) blood lineage precursor cells. The expanded cells may express CD34, SSEA-4, and HLA-II. Compositions of the expanded cells are also provided.

    摘要翻译： 提供了用于获得扩增的人脐带血细胞或表达CD34的脐带组织细胞的方法。 所述方法包括在适合促进脐带细胞扩增的共培养条件下，用足量的月经细胞接种足量的人脐带血细胞或脐带组织细胞，并在培养物下与月经细胞共培养脐带细胞 支持至少两个或更多个绳索细胞群体倍增的条件。 还提供用于生长扩大的人类绳状细胞以产生集落形成单位，集落形成单位粒细胞巨噬细胞（CFU-GM），爆发形成单位红细胞（BFU-E）和集落形成单位粒细胞红细胞巨噬细胞巨核细胞 （CFU-GEMM）血谱系前体细胞。 扩增的细胞可以表达CD34，SSEA-4和HLA-II。 还提供了扩展的细胞的组合物。

    METHOD FOR PRODUCING LANGERHANS CELLS OR INTERSTITIAL DENDRITIC CELLS OR BOTH FROM CD14+ MONOCYTES
    5.
    发明申请
    METHOD FOR PRODUCING LANGERHANS CELLS OR INTERSTITIAL DENDRITIC CELLS OR BOTH FROM CD14+ MONOCYTES 审中-公开
    用于生产来自CD14 +单核细胞的兰格细胞或间质细胞的方法

    公开(公告)号：US20100297765A1

    公开(公告)日：2010-11-25

    申请号：US12851592

    申请日：2010-08-06

    申请人： Nicolas Bechetoille ,  Valerie Andre ,  Isabelle Orly ,  Eric Perrier

    发明人： Nicolas Bechetoille ,  Valerie Andre ,  Isabelle Orly ,  Eric Perrier

    IPC分类号： C12N5/0784 ,  C12N5/0786

    CPC分类号： C12N5/0639 ,  C12N5/064 ,  C12N5/0697 ,  C12N5/0698 ,  C12N2502/091 ,  C12N2502/094 ,  C12N2502/097 ,  C12N2502/1121 ,  C12N2502/1323 ,  C12N2502/243 ,  C12N2502/28 ,  C12N2503/06

    摘要： The present invention relates to a method for preparing Langerhans cells or interstitial dendritic cells or both from CD14+ monocytes stemming from the peripheral circulatory blood of a living being, wherein the method comprises differentiation of CD 14+ monocytes into either Langerhans cells, interstitial dendritic cells, or into both types of cells by placing the CD14+ monocytes in the presence of a cell environment comprising epithelial cells and/or mesenchymatous cells.The present invention also relates to cell or tissue models comprising such prepared Langerhans cells and/or interstitial dendritic cells, and optionally macrophages and endothelial cells, and to the uses of such cell or tissue models.

    摘要翻译： 本发明涉及一种从活体的外周循环血中产生的CD14 +单核细胞或两者从朗姆汉斯细胞或间质树突状细胞制备的方法，其中所述方法包括将CD14 +单核细胞分为朗格汉斯细胞，间质树突状细胞， 或通过在包含上皮细胞和/或间充质细胞的细胞环境的存在下放置CD14 +单核细胞来进入两种类型的细胞。 本发明还涉及包含这种制备的朗格汉斯细胞和/或间质树突状细胞，以及任选的巨噬细胞和内皮细胞的细胞或组织模型，以及这种细胞或组织模型的用途。

    Alternative compositions and methods for the culture of stem cells
    6.
    发明申请
    Alternative compositions and methods for the culture of stem cells 有权
    干细胞培养的替代组合物和方法

    公开(公告)号：US20050037488A1

    公开(公告)日：2005-02-17

    申请号：US10486408

    申请日：2002-08-06

    申请人： Maisam Mitalipova ,  Ian Lyons

    发明人： Maisam Mitalipova ,  Ian Lyons

    IPC分类号： A61K35/12 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/08

    CPC分类号： C12N5/0606 ,  A61K35/12 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2502/1335 ,  C12N2502/1394 ,  C12N2502/243

    摘要： Methods and cell culture medium for the generation of human pluripotent embryonic stem cells are disclosed. Human embryonic stem cells are cultured with human granulosa feeder cells, muscle cells, Fallopian ductal epithelial cells, bone marrow stromal cells, and skin fibroblasts and the embryonic stem cells maintain their pluripotent phenotype. The human pluripotent embryonic stem cells can be cultured without feeder cells, and in the presence of supplemental growth factors. The human pluripotent embryonic stem cells can be alternatively cultured with conditioned medium obtained from a cell culture capable of maintaining human embryonic stem cells in a pluripotent state, wherein the cell culture is a human granulosa cell culture.

    摘要翻译： 公开了用于产生人多能胚胎干细胞的方法和细胞培养基。 人胚胎干细胞与人粒细胞饲养细胞，肌肉细胞，输卵管上皮细胞，骨髓基质细胞和皮肤成纤维细胞一起培养，胚胎干细胞维持其多能表型。 人类多能胚胎干细胞可以在没有饲养细胞的条件下培养，并且在补充生长因子的存在下进行培养。 可以将人多能胚胎干细胞与来自能够维持多能状态的人胚胎干细胞的细胞培养物的条件培养基一起培养，其中细胞培养物是人粒细胞培养物。

    Methods for the culture of human embryonic stem cells on human feeder cells
    8.
    发明授权
    Methods for the culture of human embryonic stem cells on human feeder cells 有权
    人胚胎干细胞培养人饲养细胞的方法

    公开(公告)号：US07432104B2

    公开(公告)日：2008-10-07

    申请号：US10486408

    申请日：2002-08-06

    申请人： Maisam Mitalipova ,  Ian Lyons

    发明人： Maisam Mitalipova ,  Ian Lyons

    IPC分类号： C12N5/02 ,  C12N5/08

    CPC分类号： C12N5/0606 ,  A61K35/12 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2502/1335 ,  C12N2502/1394 ,  C12N2502/243

    摘要： Methods and cell culture medium for the generation of human pluripotent embryonic stem cells are disclosed. Human embryonic stem cells are cultured with human granulosa feeder cells, muscle cells, Fallopian ductal epithelial cells, bone marrow stromal cells, and skin fibroblasts and the embryonic stem cells maintain their pluripotent phenotype. The human pluripotent embryonic stem cells can be cultured without feeder cells, and in the presence of supplemental growth factors. The human pluripotent embryonic stem cells can be alternatively cultured with conditioned medium obtained from a cell culture capable of maintaining human embryonic stem cells in a pluripotent state, wherein the cell culture is a human granulosa cell culture.

    摘要翻译： 公开了用于产生人多能胚胎干细胞的方法和细胞培养基。 人胚胎干细胞与人粒细胞饲养细胞，肌肉细胞，输卵管上皮细胞，骨髓基质细胞和皮肤成纤维细胞一起培养，胚胎干细胞维持其多能表型。 人类多能胚胎干细胞可以在没有饲养细胞的条件下培养，并且在补充生长因子的存在下进行培养。 可以将人多能胚胎干细胞与来自能够维持多能状态的人胚胎干细胞的细胞培养物的条件培养基一起培养，其中细胞培养物是人粒细胞培养物。

    Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium
    10.
    发明授权
    Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium 失效
    用于体外培养猪胚胎的培养基和使用培养基体外生产猪胚胎的方法

    公开(公告)号：US07060496B2

    公开(公告)日：2006-06-13

    申请号：US10790113

    申请日：2004-03-02

    申请人： Kazuhiro Kikuchi ,  Hiroyuki Kaneko ,  Junko Noguchi

    发明人： Kazuhiro Kikuchi ,  Hiroyuki Kaneko ,  Junko Noguchi

    IPC分类号： C12N5/06 ,  C12N5/00

    CPC分类号： C12N5/0604 ,  C12N2500/30 ,  C12N2502/243

    摘要： The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along with a method for in vitro culturing in vitro-produced porcine embryo using the culture medium, which can improve the quality of the resulting blastocyst and can develop the blastocyst into fetus and infant after transfer.A culture medium for the in vitro culture of in vitro-produced porcine embryo, which contains lactic acid and pyruvic acid; a culture medium for the in vitro culture, which is conditioned with oviductal epithelial cell; and a method for in vitro culturing in vitro-produced porcine embryo, comprising culturing the in vitro-produced porcine embryo using the culture medium for the 0 to 2-day term after fertilization and subsequently culturing the embryo in a glucose-containing culture medium.

    摘要翻译： 本发明提供了用于体外培养的体外培养的猪胚胎的体外培养基，其可以改善所产生的囊胚的质量，并且可以提高转移后胎儿和婴儿的发育比例以及方法 用于体外培养体外产生的使用培养基的猪胚胎，其可以改善所产生的囊胚的质量，并且可以在转移后将囊胚发育成胎儿和婴儿。 用于体外培养体外培养的含有乳酸和丙酮酸的猪胚胎的培养基; 用于体外培养的培养基，其用输卵管上皮细胞调节; 以及体外培养体外产生的猪胚胎的方法，包括使用培养基培养体外产生的猪胚胎，在受精后0至2天，随后在含葡萄糖的培养基中培养胚胎。