公开(公告)号：US07943821B2

公开(公告)日：2011-05-17

申请号：US11991922

申请日：2006-03-20

申请人： Kazuko Shinozaki ,  Masayuki Kakimoto ,  Feng Qin ,  Yoh Sakuma ,  Kyonoshin Maruyama

发明人： Kazuko Shinozaki ,  Masayuki Kakimoto ,  Feng Qin ,  Yoh Sakuma ,  Kyonoshin Maruyama

IPC分类号： C12N15/82 ,  C12N15/29 ,  A01H5/00 ,  C07K14/415

CPC分类号： C07K14/415 ,  C12N15/8273

摘要： This invention relates to a stress-inducible transcription factor derived from maize, a gene encoding the same, and a method for using the same. Specifically, this invention provides a gene comprising the following DNA (a) or (b): (a) DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1; or (b) maize-derived DNA hybridizing under stringent conditions with DNA consisting of a nucleotide sequence that is complementary to the DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1 and encoding a protein that regulates the transcription of a gene located downstream of a stress responsive element. Further, this invention relates to a transgenic plant having improved tolerance to environmental stress, such as high-temperature or dehydration stress, into which such gene has been introduced.

摘要翻译： 本发明涉及来自玉米的应激诱导型转录因子，其编码基因的转录因子及其使用方法。 具体地，本发明提供包含以下DNA（a）或（b）的基因：（a）由SEQ ID NO：1所示的核苷酸序列组成的DNA; 或（b）在严格条件下与由与由SEQ ID NO：1所示的核苷酸序列组成的DNA互补的核苷酸序列的DNA杂交的玉米衍生的DNA，并编码调节位于 在应力响应元件的下游。 此外，本发明涉及具有改善的耐受这种基因导入的高温或脱水应激的环​​境胁迫的转基因植物。

公开(公告)号：US20090307794A1

公开(公告)日：2009-12-10

申请号：US11991922

申请日：2006-03-20

申请人： Kazuko Shinozaki ,  Masayuki Kakimoto ,  Feng Qin ,  Yoh Sakuma ,  Kyonoshin Maruyama

发明人： Kazuko Shinozaki ,  Masayuki Kakimoto ,  Feng Qin ,  Yoh Sakuma ,  Kyonoshin Maruyama

IPC分类号： A01H1/00 ,  C07H21/00 ,  C07K14/415 ,  C12N15/63 ,  C12N5/04 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8273

摘要： This invention relates to a stress-inducible transcription factor derived from maize, a gene encoding the same, and a method for using the same. Specifically, this invention provides a gene comprising the following DNA (a) or (b): (a) DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1; or (b) maize-derived DNA hybridizing under stringent conditions with DNA consisting of a nucleotide sequence that is complementary to the DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1 and encoding a protein that regulates the transcription of a gene located downstream of a stress responsive element. Further, this invention relates to a transgenic plant having improved tolerance to environmental stress, such as high-temperature or dehydration stress, into which such gene has been introduced.

摘要翻译： 本发明涉及来自玉米的应激诱导型转录因子，其编码基因的转录因子及其使用方法。 具体地，本发明提供包含以下DNA（a）或（b）的基因：（a）由SEQ ID NO：1所示的核苷酸序列组成的DNA; 或（b）在严格条件下与由与由SEQ ID NO：1所示的核苷酸序列组成的DNA互补的核苷酸序列的DNA杂交的玉米衍生的DNA，并编码调节位于 在应力响应元件的下游。 此外，本发明涉及具有改善的耐受这种基因导入的高温或脱水应激的环​​境胁迫的转基因植物。

公开(公告)号：US20090089899A1

公开(公告)日：2009-04-02

申请号：US12084346

申请日：2006-03-15

申请人： Kazuko Shinozaki ,  Yasunari Fujita ,  Kyonoshin Maruyama

发明人： Kazuko Shinozaki ,  Yasunari Fujita ,  Kyonoshin Maruyama

IPC分类号： C12N15/29 ,  C12N15/82

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： Provided are: active AREB1 capable of improving plant tolerance against environmental stresses such as drought, salt, and low temperature through expression at high levels in plant bodies; and a method for improving plant environmental stress tolerance using the active AREB1. Also provided is an active environmental stress responsive transcription factor AREB1 gene lacking the activation control region of the plant environmental stress responsive transcription factor AREB1 and containing an N-terminal transcriptional activation domain and a DNA binding domain.

摘要翻译： 提供：活性AREB1能够通过在植物体中高水平表达来改善植物对抗环境胁迫（如干旱，盐和低温）的耐受性; 以及使用活性AREB1改善植物环境胁迫耐受性的方法。 还提供了缺乏植物环境应激反应转录因子AREB1的激活控制区域并且含有N末端转录激活结构域和DNA结合结构域的活性环境应激反应转录因子AREB1基因。

公开(公告)号：US07259297B2

公开(公告)日：2007-08-21

申请号：US10887475

申请日：2004-07-08

申请人： Kazuko Shinozaki ,  Mie Kasuga ,  Yoh Sakuma

发明人： Kazuko Shinozaki ,  Mie Kasuga ,  Yoh Sakuma

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8271 ,  C12N15/8273

摘要： The present invention relates to an environmental stress-tolerant plant. The invention discloses a transgenic plant containing a gene in which a DNA encodes a protein consisting of the amino acid sequence as shown in SEQ ID NO: 4 with the deletion of amino acids from positions 136-165 thereof, the DNA being operably linked downstream of a stress responsive promoter.

摘要翻译： 本发明涉及环境胁迫耐受性植物。 本发明公开了一种含有基因的转基因植物，其中DNA编码由SEQ ID NO：4所示的氨基酸序列组成的蛋白质，其缺失位于第136-165位的氨基酸，该DNA可操作地连接在 应激反应启动子。

公开(公告)号：US07842854B2

公开(公告)日：2010-11-30

申请号：US11453724

申请日：2006-06-15

申请人： Kazuko Shinozaki ,  Yusuke Ito ,  Yoh Sakuma

发明人： Kazuko Shinozaki ,  Yusuke Ito ,  Yoh Sakuma

IPC分类号： C12N15/29 ,  C12N15/82

CPC分类号： C07K14/415 ,  C07H21/04 ,  C12N15/8273

摘要： Identification of a gene from monocotyledonous plants such as rice, which codes for a transcription factor specific to a stress tolerant gene and provision of a novel environmental stress tolerant plant using the gene. From the rice genome, a gene, which binds to a cis element existing upstream of the gene encoding a stress responsive protein and for a transcription factor to activate the transcription of the gene, is identified. Further, the gene of the transcription factor is used to transform a plant, thereby improving tolerance against environmental stresses such as low temperature, dehydration, and salt stresses.

摘要翻译： 鉴定来自单子叶植物如水稻的基因，其编码对胁迫耐受基因特异性的转录因子，并提供使用该基因的新型环境胁迫耐受植物。 从水稻基因组中鉴定了与编码应激反应蛋白的基因上游存在的顺式元件和激活基因转录的转录因子结合的基因。 此外，转录因子的基因用于转化植物，从而改善对环境胁迫的耐受性，例如低温，脱水和盐胁迫。

公开(公告)号：US07138277B2

公开(公告)日：2006-11-21

申请号：US10302382

申请日：2002-11-22

申请人： Kazuko Shinozaki ,  Yusuke Ito ,  Yoh Sakuma

发明人： Kazuko Shinozaki ,  Yusuke Ito ,  Yoh Sakuma

IPC分类号： A01H5/00 ,  C12N15/82

CPC分类号： C07K14/415 ,  C07H21/04 ,  C12N15/8273

摘要： Identification of a gene from monocotyledonous plants such as rice, which codes for a transcription factor specific to a stress tolerant gene and provision of a novel environmental stress tolerant plant using the gene. From the rice genome, a gene, which binds to a cis element existing upstream of the gene encoding a stress responsive protein and for a transcription factor to activate the transcription of the gene, is identified. Further, the gene of the transcription factor is used to transform a plant, thereby improving tolerance against environmental stresses such as low temperature, dehydration, and salt stresses.

摘要翻译： 鉴定来自单子叶植物如水稻的基因，其编码对胁迫耐受基因特异性的转录因子，并提供使用该基因的新型环境胁迫耐受植物。 从水稻基因组中鉴定了与编码应激应答蛋白的基因上游存在的顺式元件和激活基因转录的转录因子结合的基因。 此外，转录因子的基因用于转化植物，从而改善对环境胁迫的耐受性，例如低温，脱水和盐胁迫。

公开(公告)号：US20060015973A1

公开(公告)日：2006-01-19

申请号：US10887475

申请日：2004-07-08

申请人： Kazuko Shinozaki ,  Mie Kasuga ,  Yoh Sakuma

发明人： Kazuko Shinozaki ,  Mie Kasuga ,  Yoh Sakuma

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8271 ,  C12N15/8273

摘要： The present invention relates to an environmental stress-tolerant plant. The invention discloses a transgenic plant containing a gene in which a DNA encoding a protein consisting of the amino acid sequence as shown in SEQ ID NO: 4, operably linked downstream of a stress responsive promoter.

摘要翻译： 本发明涉及环境胁迫耐受性植物。 本发明公开了一种含有基因的转基因植物，其中编码由SEQ ID NO：4所示的氨基酸序列组成的蛋白质的DNA，其可操作地连接在应激反应启动子的下游。

公开(公告)号：US07045355B2

公开(公告)日：2006-05-16

申请号：US10266487

申请日：2002-10-08

申请人： Kazuko Shinozaki ,  Mie Kasuga

发明人： Kazuko Shinozaki ,  Mie Kasuga

IPC分类号： C12N15/82

CPC分类号： A61K36/48 ,  C07K14/415 ,  C12N15/8273

摘要： According to the present invention, a protein which binds to DRE to activate the transcription of genes located downstream of the DRE, a gene encoding the protein, a recombinant vector comprising the gene, a transformant comprising the recombinant vector, a transgenic plant comprising the gene, and a method for producing the gene using the transformant are provided. The present invention is useful for creating a stress tolerant plant.

公开(公告)号：US20060195949A1

公开(公告)日：2006-08-31

申请号：US10664741

申请日：2003-09-19

申请人： Kazuko Shinozaki ,  Mie Kasuga

发明人： Kazuko Shinozaki ,  Mie Kasuga

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8237 ,  C12N15/8271 ,  C12N15/8273

摘要： The present invention relates to an environmental stress-tolerant plant. The invention discloses a transgenic plant containing a gene in which a DNA encoding the following protein (a) or (b) is ligated downstream of a stress responsive promoter: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8 or SEQ ID NO: 10; (b) a protein which consists of the amino acid sequence having deletion, substitution or addition of at least one amino acid in the amino acid sequence as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8 or SEQ ID NO: 10 and which regulates the transcription of genes located downstream of a stress responsive element.

摘要翻译： 本发明涉及环境胁迫耐受性植物。 本发明公开了一种含有基因的转基因植物，其中将编码下列蛋白质（a）或（b）的DNA连接到胁迫应激启动子的下游：（a）由如SEQ ID NO所示的氨基酸序列组成的蛋白质 ：2，SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8或SEQ ID NO：10; （b）由如SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6所示的氨基酸序列中具有至少一个氨基酸的缺失，取代或添加的氨基酸序列组成的蛋白质， SEQ ID NO：8或SEQ ID NO：10，其调节位于应激反应元件下游的基因的转录。

公开(公告)号：US20050278799A1

公开(公告)日：2005-12-15

申请号：US10391414

申请日：2003-03-18

申请人： Kazuko Shinozaki ,  Koji Katsura ,  Yusuke Ito

发明人： Kazuko Shinozaki ,  Koji Katsura ,  Yusuke Ito

IPC分类号： C12N15/29 ,  A01H1/00 ,  A01H5/00 ,  C12N5/04 ,  C12N15/11 ,  C12N15/52 ,  C12N15/63 ,  C12N15/66 ,  C12N15/82

CPC分类号： C12N15/8237

摘要： The invention relates to an isolated stress-induced promoter which effectively functions in monocotyledonous plants such as rice, and of environmental stress tolerant plants using the promoter.

摘要翻译： 本发明涉及在单子叶植物如水稻和使用该启动子的环境胁迫耐受植物中有效起作用的分离的应激诱导的启动子。