公开(公告)号：US20100035291A1

公开(公告)日：2010-02-11

申请号：US12507440

申请日：2009-07-22

申请人： Kenji MIYAZAKI ,  Yo Tabuse ,  Hiroko Someya ,  Wataru Hattori ,  Hisao Kawaura

发明人： Kenji MIYAZAKI ,  Yo Tabuse ,  Hiroko Someya ,  Wataru Hattori ,  Hisao Kawaura

IPC分类号： C12Q1/37 ,  C12N9/50 ,  C12M1/00 ,  G01N27/00

CPC分类号： C07K5/0812 ,  C07K1/12 ,  C07K5/0821

摘要： A fragmenting reaction of peptide is achieved while maintaining the isolated state of peptide. Isolated peptide fractions isolated by electrophoresis are prepared in flow paths. Subsequently, prepared peptide fractions are dried by each of the flow paths. Then, dried peptide fractions are in contact with protease. Then, independent liquid membranes of a solvent are formed over the surfaces of peptide fractions, which have been in contact with protease, disposed on the flow paths, respectively.

摘要翻译： 在保持肽的分离状态的同时实现肽的片段化反应。 在流路中制备通过电泳分离的分离的肽级分。 随后，通过每个流动路径干燥制备的肽级分。 然后，干燥的肽级分与蛋白酶接触。 然后，在分别设置在流路上的与肽蛋白酶接触的肽部分的表面上分别形成溶剂的独立液膜。

公开(公告)号：US20070026456A1

公开(公告)日：2007-02-01

申请号：US10570205

申请日：2004-09-02

申请人： Kenichi Kamijo ,  Hisao Kawaura ,  Toru Sano ,  Yo Tabuse ,  Hirotaka Minagawa ,  Kenji Miyazaki

发明人： Kenichi Kamijo ,  Hisao Kawaura ,  Toru Sano ,  Yo Tabuse ,  Hirotaka Minagawa ,  Kenji Miyazaki

IPC分类号： G01N33/53

CPC分类号： G01N33/6803

摘要： A biomolecule is analyzed with high accuracy. A biomolecule is analyzed with high likelihood. A biomolecule as an analysis subject is modified with a marker binding or adsorbing to only its specific portion (S101). Next, the biomolecule modified with the marker is developed on a base plate (S102). The arrangement of the biomolecule on the base plate is detected using a marker (S103). Then, scanning is performed along the shape of the biomolecule present on the detected position (S104). The biomolecule is analyzed based on an information relating to the shape or arrangement of the biomolecule obtained by scanning or an information relating to the arrangement of the marker on the biomolecule (S105).

摘要翻译： 以高精度分析生物分子。 分析生物分子的可能性很高。 作为分析对象的生物分子用仅结合或吸附到其特定部分的标记进行修饰（S 101）。 接下来，在基板上显影用标记物修饰的生物分子（S102）。 使用标记检测基板上的生物分子的排列（S103）。 然后，沿检测位置上存在的生物分子的形状进行扫描（S104）。 基于与通过扫描获得的生物分子的形状或排列相关的信息或与生物分子上的标记的排列有关的信息来分析生物分子（S105）。

公开(公告)号：US08207301B2

公开(公告)日：2012-06-26

申请号：US12452216

申请日：2008-06-16

申请人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

发明人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

IPC分类号： C07K14/435

CPC分类号： C07K14/4748 ,  G01N33/57438

摘要： Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.

摘要翻译： 提供：与非肝细胞癌细胞相比，通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。 用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO：1表示的氨基酸形成的肿瘤排斥抗原gp96，并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

公开(公告)号：US20100248256A1

公开(公告)日：2010-09-30

申请号：US12452216

申请日：2008-06-16

申请人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

发明人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

IPC分类号： G01N33/53 ,  C07K14/435

CPC分类号： C07K14/4748 ,  G01N33/57438

摘要： Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.

摘要翻译： 提供：与非肝细胞癌细胞相比，通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。 用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO：1表示的氨基酸形成的肿瘤排斥抗原gp96，并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

公开(公告)号：US20120225440A1

公开(公告)日：2012-09-06

申请号：US13471629

申请日：2012-05-15

申请人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

发明人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

IPC分类号： G01N33/574 ,  G01N27/62 ,  G01N21/64

CPC分类号： C07K14/4748 ,  G01N33/57438

摘要： A method of detecting hepatocellular carcinoma includes using an isolated protein including an amino acid sequence represented by SEQ ID NO: 1.

摘要翻译： 检测肝细胞癌的方法包括使用包含SEQ ID NO：1所示氨基酸序列的分离蛋白。

公开(公告)号：US20110053196A1

公开(公告)日：2011-03-03

申请号：US12278711

申请日：2007-02-08

申请人： Kenji Miyazaki ,  Yo Tabuse ,  Hirotaka Minagawa ,  Emiko Saito ,  Ken'ichi Kamijo ,  Akira Tsugita ,  Kozue Tsugita

发明人： Kenji Miyazaki ,  Yo Tabuse ,  Hirotaka Minagawa ,  Emiko Saito ,  Ken'ichi Kamijo ,  Akira Tsugita ,  Kozue Tsugita

IPC分类号： C12Q1/37 ,  C07K1/113

CPC分类号： G01N33/6803 ,  C07K1/107

摘要： The present invention is to provide a method for easily and specifically modifying specific amino acid residue(s) constituting a peptide and to provide a methodology of improving the accuracy of identification of the peptide using a new information of the peptide obtained from the number of modified amino acid residue by said specific modification method as mentioned. The method for modifying a peptide according to the present invention is characterized:A method for modifying a peptide, wherein the peptide as supported in a substrate and an aqueous solution of perhalogenated carboxylic acid containing an alcohol is reacted to selectively esterify a glutamic acid residue of said peptide.The method for identifying a peptide according to the present invention is characterized:A method for identifying a peptide comprising the steps of: reacting the peptide as supported in a substrate and an aqueous solution of perhalogenated carboxylic acid containing an alcohol to selectively esterify glutamic acid residue of said peptide; immersing said substrate in a protease solution to obtain a peptide fragment originated from said peptide; measuring a molecular weight of said peptide fragment; and determining said peptide based on said molecular weight.

摘要翻译： 本发明提供一种容易且特异性地修饰构成肽的特定氨基酸残基的方法，并且提供使用从修饰的数量获得的肽的新信息来提高肽的鉴定准确度的方法 通过所述具体的改性方法制备氨基酸残基。 本发明的肽的修饰方法的特征在于：对肽进行修饰的方法，其中负载在底物中的肽和含有醇的全卤代羧酸的水溶液进行反应以选择性地酯化谷氨酸残基 所述肽。 本发明鉴定肽的方法的特征在于：一种鉴定肽的方法，包括以下步骤：将载体载于底物中的肽与含有醇的全卤代羧酸水溶液反应以选择性酯化谷氨酸残基 的所述肽; 将所述底物浸入蛋白酶溶液中以获得源自所述肽的肽片段; 测量所述肽片段的分子量; 并基于所述分子量测定所述肽。